Functionalization of hybrid surface microparticles for in vitro cellular antigen classification

Ashley, Brandon K.; Sui, Jianye; Javanmard, Mehdi; Hassan, Umer

doi:10.1007/s00216-020-03026-4

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…Another limitation is that SMA cannot distinguish competitive analyte species with similar pulse frequencies or exaggerate their amplitude differences, treating all objects which are counted with equal scrutiny. To better differentiate two or more materials, other phenotypic properties must be exploited (e.g., measure more electrically sensitive particles, probe particles at different input frequencies, or use functionalized particles for receptor attachment and identification) (Ashley et al, 2021; Prakash et al, 2020; Sui et al, 2020). Finally, the maximum SNR achieved would reach a climax and begin to decline from increasing number of data points used in the SMA algorithm.…”

Section: Discussionmentioning

confidence: 99%

Time‐domain signal averaging to improve microparticles detection and enumeration accuracy in a microfluidic impedance cytometer

Ashley

Hassan

2021

Biotech & Bioengineering

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Microfluidic impedance cytometry is a powerful system to measure micro and nano-sized particles and is routinely used in point-of-care disease diagnostics and other biomedical applications. However, small objects near a sensor's detection limit are plagued with relatively significant background noise and are difficult to identify for every case. While many data processing techniques can be utilized to reduce noise and improve signal quality, frequently they are still inadequate to push sensor detection limits. Here, we report the first demonstration of a novel signal averaging algorithm effective in noise reduction of microfluidic impedance cytometry data, improving enumeration accuracy, and reducing detection limits. Our device uses a 22 µm tall × 100 µm wide (with 30 µm wide focused aperture) microchannel and gold coplanar microelectrodes that generate an electric field, recording bipolar pulses from polystyrene microparticles flowing through the channel. In addition to outlining a modified moving signal averaging technique theoretically and with a model data set, we also performed a compendium of characterization experiments including variations in flow rate, input voltage, and particle size. Multivariate metrics from each experiment are compared including signal amplitude, pulse width, background noise, and signal-to-noise ratio (SNR). Incorporating our technique resulted in improved SNR and counting accuracy across all experiments conducted, and the limit of detection improved from 5 to 1 µm particles without modifying microchannel dimensions.Succeeding this, we envision implementing our modified moving average technique to develop next-generation microfluidic impedance cytometry devices with an expanded dynamic range and improved enumeration accuracy. This can be exceedingly useful for many biomedical applications, such as infectious disease diagnostics where devices may enumerate larger-scale immune cells alongside sub-micron bacterium in the same sample.

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Section: Discussionmentioning

confidence: 99%

Time‐domain signal averaging to improve microparticles detection and enumeration accuracy in a microfluidic impedance cytometer

Ashley

Hassan

2021

Biotech & Bioengineering

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“…Like SAv purification following incubation, the solution was washed with the same conditions and parameters to purify MOJPs and separate out unbound antibodies (i.e., centrifugation, supernatant aspiration, pellet resuspension in 1× PBS, etc.). 48…”

Section: Signal Acquisition Processing and Sampling Algorithmmentioning

confidence: 99%

Antibody-functionalized aluminum oxide-coated particles targeting neutrophil receptors in a multifrequency microfluidic impedance cytometer

et al. 2022

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“…Microfluidics provide a compartmentalized scaffold which may perform optimized and regulated sample processing as well as identify and separate biomarkers based on defined characteristics found at micro or nanoscale dimensions (Boneschansker et al, 2014; Brown et al, 2018; Ellett et al, 2018). In microfluidics, nanoparticles are the active agents performing and displaying the analytical changes in the assays, owing to their heterogenous material origins and functionalization including magneto‐nanoparticles, quantum dots (QDs), electrically tuned particles, barcoded particles, and others (Ashley et al, 2020; Cihalova et al, 2017; Mok et al, 2014; Uddin et al, 2016). Our own work studies metal oxide‐coated hybrid surface microparticles with unique impedance measurements within a microfluidic impedance sensing system to target leukocyte receptors related to sepsis such as CD11b, CD64, and CD66b (Ashley et al, 2020; Xie et al, 2017).…”

Section: M/n Technologies For Sepsis Biomarkersmentioning

confidence: 99%

Point‐of‐critical‐care diagnostics for sepsis enabled by multiplexed micro and nanosensing technologies

Ashley

Hassan

2021

WIREs Nanomed Nanobiotechnol

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Sepsis is responsible for the highest economic and mortality burden in critical care settings around the world, prompting the World Health Organization in 2018 to designate it as a global health priority. Despite its high universal prevalence and mortality rate, a disproportionately low amount of sponsored research funding is directed toward diagnosis and treatment of sepsis, when early treatment has been shown to significantly improve survival. Additionally, current technologies and methods are inadequate to provide an accurate and timely diagnosis of septic patients in multiple clinical environments. For improved patient outcomes, a comprehensive immunological evaluation is critical which is comprised of both traditional testing and quantifying recently proposed biomarkers for sepsis. There is an urgent need to develop novel point‐of‐care, low‐cost systems which can accurately stratify patients. These point‐of‐critical‐care sensors should adopt a multiplexed approach utilizing multimodal sensing for heterogenous biomarker detection. For effective multiplexing, the sensors must satisfy criteria including rapid sample to result delivery, low sample volumes for clinical sample sparring, and reduced costs per test. A compendium of currently developed multiplexed micro and nano (M/N)‐based diagnostic technologies for potential applications toward sepsis are presented. We have also explored the various biomarkers targeted for sepsis including immune cell morphology changes, circulating proteins, small molecules, and presence of infectious pathogens. An overview of different M/N detection mechanisms are also provided, along with recent advances in related nanotechnologies which have shown improved patient outcomes and perspectives on what future successful technologies may encompass. This article is categorized under: Diagnostic Tools > Biosensing

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Functionalization of hybrid surface microparticles for in vitro cellular antigen classification

Cited by 6 publications

References 46 publications

Time‐domain signal averaging to improve microparticles detection and enumeration accuracy in a microfluidic impedance cytometer

Time‐domain signal averaging to improve microparticles detection and enumeration accuracy in a microfluidic impedance cytometer

Antibody-functionalized aluminum oxide-coated particles targeting neutrophil receptors in a multifrequency microfluidic impedance cytometer

Point‐of‐critical‐care diagnostics for sepsis enabled by multiplexed micro and nanosensing technologies

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