Functionalization of Se-Te Nanorods with Au Nanoparticles for Enhanced Anti-Bacterial and Anti-Cancer Activities

Abstract

The use of medical devices for therapeutic and diagnostic purpose is globally increasing; however, bacterial colonization on therapeutic devices can occur, causing severe infections in the human body. It has become an issue for public health. It is necessary to develop a nanomaterial based on photothermal treatment to kill toxic bacterial strains. Appropriately, high photothermal conversion and low-cost powerful photothermal agents have been investigated. Recently, gold nanocomposites have attracted great interest in biological applications. Here, we prepared rod-shaped Se-Te@Au nanocomposites of about 200 nm with uniform shape and surface-coated with gold nanoparticles for the first time showing high anti-bacterial and anti-cancer activities. Se-Te@Au showed proper structural consistency and natural resistance to bacterial and cancer cells. The strong absorption and high photothermal conversion efficacy made it a good photothermal agent material for the photothermal treatment of bacterial and cancer cells. The Se-Te@Au rod showed excellent anti-bacterial efficacy against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, with highest recorded inhibition zones of 25 ± 2 mm and 22 ± 2 mm, respectively. More than 99% of both types of strains were killed after 5 min with a near-infrared (NIR) laser at the very low concentration of 48 µg/mL. The Se-Te@Au rod’s explosion in HeLa cells was extensively repressed and demonstrated high toxicity at 100 µg/mL for 5 min when subjected to an NIR laser. As a result of its high photothermal characteristics, the exceptional anti-bacterial and anti-cancer effects of the Se-Te@Au rod are considerably better than those of other methods previously published in articles. This study could open a new framework for sterilization applications on the industrial level.

NIR light-activated nanocomposites combat biofilm formation and enhance antibacterial efficacy for improved wound healing

“…2C ) 31 . The prominent diffraction peak at 28.77° indicates that cetyltrimethylammonium bromide (CTAB) directs the formation of Se-Te NPs on the (101) lattice plane 32 , 33 . Furthermore, the CuO NPs were immobilized on the surface of the Se-Te NPs.…”

Silver incorporated SeTe nanoparticles with enhanced photothermal and photodynamic properties for synergistic effects on anti-bacterial activity and wound healing

“…The prominent diffraction peak at 2q = 28.77°shows that CTAB directs the growth of SeTe NPs in the (101) lattice plane reference. 29 3.1.3. X-ray photoelectron spectroscopy (XPS).…”

“…S2 † shows Se 3d spectra having two different bands at 55.1 eV (Se 3d 3/2 ) and 54.2 eV (Se 3d 5/2 ), while a broad band observed around 60.2 eV is assigned to the SeO 2 bond in SeTe-Ag NPs. 29 3.1.4. Fourier-transform infrared spectroscopy.…”

“…To find the growth curve pattern of bacteria under the influence of NPs, bacteria with an OD of 0.4 were treated with different concentrations of NPs and cultured for 10 h, and OD 600 was recorded every hour. 29 Furthermore, for antibiofilm assay, bacterial culture of OD 600 , 0.025, was added to 96 well plate and grown for 24 h at 37 °C. When the biofilms were formed on the walls, the media was removed by gently inverting the plate, and PBS was used to wash the biofilm and remove the planktonic cells.…”

“…The results show that the bactericidal activity of NPs increased with an increase in concentration. 29 3.3.4. Agar plate method.…”