Functionalization of silicon oxide using supercritical fluid deposition of 3,4-epoxybutyltrimethoxysilane for the immobilization of amino-modified oligonucleotide

Rull, Jordi; Nonglaton, Guillaume; Costa, Guillaume; Fontelaye, Caroline; Marchi-Delapierre, Caroline; Ménage, Stéphane; Marchand, Gilles

doi:10.1016/j.apsusc.2015.06.168

Cited by 8 publications

(5 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After emulsion graft polymerization, the surface hydrophilicity of the COC film was significantly improved, and the WCA decreased to 58°, which is more suitable for probe immobilization. Because the grafted nanoparticle layer contained many functional epoxide groups, oligonucleotides (probe A, Table ) with amino groups were immobilized on the surface-modified COC through a nucleophilic ring-opening reaction. , Here, we utilized Cy3 as a fluorescence label of the oligonucleotide probe to evaluate their immobilization properties on the substrate. The probe solutions with concentrations of 0.25, 0.5, 1.0, 2.0, and 5.0 μM were spotted on the surface-modified COC.…”

Section: Resultsmentioning

confidence: 99%

Highly Transparent Cyclic Olefin Copolymer Film with a Nanotextured Surface Prepared by One-Step Photografting for High-Density DNA Immobilization

Yuan¹,

Wang²,

Zhao³

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Compared with conventional glass slides and two-dimensional (2D) planar microarrays, polymer-based support materials and three-dimensional (3D) surface structures have attracted increasing attention in the field of biochips because of their good processability in microfabrication and low cost in mass production, as well as their improved sensitivity and specificity for the detection of biomolecules. In the present study, UV-induced emulsion graft polymerization was carried out on a cyclic olefin copolymer (COC) surface to generate 3D nanotextures composed of loosely stacked nanoparticles with a diameter of approximately 50 nm. The introduction of a hierarchical nanostructure on a COC surface only resulted in a 5% decrease in its transparency at a wavelength of 550 nm but significantly increased the surface area, which markedly improved immobilization density and efficiency of an oligonucleotide probe compared with the functional group and polymer brush-modified substrates. The highest immobilization efficiency of the probes reached 93%, and a limit of detection of 75 pM could be obtained. The hybridization experiment demonstrated that the 3D gene chip exhibited excellent sensitivity for target DNA detection and single-nucleotide polymorphism discrimination. This one-step approach to the construction of nanotextured surfaces on the COC has promising applications in the fields of biochips and immunoassays.

show abstract

Section: Resultsmentioning

confidence: 99%

Highly Transparent Cyclic Olefin Copolymer Film with a Nanotextured Surface Prepared by One-Step Photografting for High-Density DNA Immobilization

Yuan¹,

Wang²,

Zhao³

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…The PEGDA acts as a crosslinker, its PEG part offers appropriate hydrophilicity to the crosslinked network, and GMA was selected as a functional comonomer providing high reactive epoxide groups for subsequent immobilization of oligonucleotide probes through a nucleophilic ring-opening reaction. 29,30 The chemical structure of ther hydrogel layer was characterized by ATR-FTIR measurements. Figure 1 shows the infrared spectra of hydrogel film with PEGDA:GMA monomer mass ratios of 1:1 and 1:0.…”

Section: Resultsmentioning

confidence: 99%

Preparation of a poly (PEGDA‐co‐GMA) thin hydrogel matrix for oligonucleotide microarray applications

Yuan

Zhao

et al. 2021

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: Fabricating oligonucleotide microarrays with high immobilization efficiency and sensitivity by a simple surface modification route remains highly desirable in the biochip field. Here we develop a facile method to prepare a 3D crosslinking thin hydrogel film on glass slides for high-density immobilization of DNA probes. RESULTS: Thin hydrogel film using poly (ethylene glycol) diacrylate (PEGDA) as crosslinker and glycidyl methacrylate (GMA) as functional monomer was attached on vinyl group-modified glass slides by a photopolymerization technique. The effect of monomer ratios (PEGDA:GMA) on the surface hydrophilicity of the hydrogel layer and the immobilization density of DNA probes were investigated and an optimal monomer ratio of 10:1 and te maximum immobilization density of 1.4 pmol cm -2 were found. Adjusting the hydrophilicity of the hydrogel film effectively suppressed its swelling and produced a typical dense but not porous structure with a flat surface favorable for printing microarrays. The maximum immobilization density and efficiency of oligonucleotide probes on the 3D hydrogel film were 1.4 pmol cm -2 and 83%, respectively, which are superior values to those on a 1D support. The microarray had a wide detection range of target nucleotides from 15.3 pmol L -1 to 62.5 nmol L -1 . In addition, the hybridization assay demonstrated that the microarray showed high sensitivity with a limit of detection of 30.5 pmol L -1 . CONCLUSION:The poly (PEGDA-co-GMA) hydrogel film could be grafted on the glass slide by the photopolymerization technique and the DNA probe was effectively immobilized on the thin hydrogel by reacting with the epoxy groups. This strategy of attaching a thin cross-linked poly(PEGDA-co-GMA) hydrogel layer on glass slides has promising prospects in the fields of bioanalysis and microarrays.

show abstract

“…Prior to the SFD and in order to clean and activate the surface, an oxygen plasma pretreatment was performed (1000 mL min −1 of O 2 , 800 W, 5 min, Tepla 300 system). The employed SFD process, reported in detail elsewhere, can be resumed as follow: the chamber was pressurized at 12 MPa; 150 µL of 1% (v/v) MAPTMS solution in ethanol was injected 10 times during 5 min under a constant flow of 50 g min −1 of supercritical CO 2 ; the chamber was then kept at 12 MPa for 15 min to ensure the crosslinking of MAPTMS with the substrate; finally, a constant flow of 100 g min −1 of supercritical CO 2 was used to clean the surface sample and to remove all molecules precursors that did not react. After depressurizing the chamber, the samples were heated at 110 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Multiphoton Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization

Accardo

Blatché

Courson

et al. 2017

Small

View full text Add to dashboard Cite

The realization of 3D architectures for the study of cell growth, proliferation, and differentiation is a task of fundamental importance for both technological and biological communities involved in the development of biomimetic cell culture environments. Here we report the fabrication of 3D freestanding scaffolds, realized by multiphoton direct laser writing and seeded with neuroblastoma cells, and their multitechnique characterization using advanced 3D fluorescence imaging approaches. The high accuracy of the fabrication process (≈200 nm) allows a much finer control of the micro- and nanoscale features compared to other 3D printing technologies based on fused deposition modeling, inkjet printing, selective laser sintering, or polyjet technology. Scanning electron microscopy (SEM) provides detailed insights about the morphology of both cells and cellular interconnections around the 3D architecture. On the other hand, the nature of the seeding in the inner core of the 3D scaffold, inaccessible by conventional SEM imaging, is unveiled by light sheet fluorescence microscopy and multiphoton confocal imaging highlighting an optimal cell colonization both around and within the 3D scaffold as well as the formation of long neuritic extensions. The results open appealing scenarios for the use of the developed 3D fabrication/3D imaging protocols in several neuroscientific contexts.

show abstract

Functionalization of silicon oxide using supercritical fluid deposition of 3,4-epoxybutyltrimethoxysilane for the immobilization of amino-modified oligonucleotide

Cited by 8 publications

References 97 publications

Highly Transparent Cyclic Olefin Copolymer Film with a Nanotextured Surface Prepared by One-Step Photografting for High-Density DNA Immobilization

Highly Transparent Cyclic Olefin Copolymer Film with a Nanotextured Surface Prepared by One-Step Photografting for High-Density DNA Immobilization

Preparation of a poly (PEGDA‐co‐GMA) thin hydrogel matrix for oligonucleotide microarray applications

Multiphoton Direct Laser Writing and 3D Imaging of Polymeric Freestanding Architectures for Cell Colonization

Contact Info

Product

Resources

About