Functionalization of Thioctic Acid-Capped Gold Nanoparticles for Specific Immobilization of Histidine-Tagged Proteins

Abad, José M.; Mertens, Stijn F. L.; Pita, Marcos; Fernández, Vı́ctor M.; Schiffrin, David J.

doi:10.1021/ja042717i

Cited by 254 publications

(200 citation statements)

References 59 publications

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“…This concept can be employed to manipulate the histidine-tagged recombinant proteins and bind other biological substrates at low concentrations. [32,33] In analogy to proteins, nanoparticles can be used as a multivalent receptor to enhance low-affinity interactions such as carbohydrate-protein interactions. [34] As an example, Lin et al prepared mannose-functionalized gold nanoparticles and investigated their interaction with the lectin, concanavalin A (Con A).…”

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confidence: 99%

Applications of Nanoparticles in Biology

2008

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Applications of Nanoparticles in Biology

2008

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“…The concentrations investigated are in the range of LA ligands observed on the surface of gold nanospheres. 63 In simulations, ligands are connected to opposing faces of a Au (111) slab at 11, 15, and 20 Å nanogap separations, d Au . Simulation parameters are further detailed in the Methods Section.…”

Section: Resultsmentioning

confidence: 99%

Driving Chemical Reactions in Plasmonic Nanogaps with Electrohydrodynamic Flow

Thrift

Nguyen

Darvishzadeh-Varcheie

et al. 2017

ACS Nano

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Nanoparticles from colloidal solutionwith controlled composition, size, and shapeserve as excellent building blocks for plasmonic devices and metasurfaces. However, understanding hierarchical driving forces affecting the geometry of oligomers and interparticle gap spacings is still needed to fabricate high-density architectures over large areas. Here, electrohydrodynamic (EHD) flow is used as a long-range driving force to enable carbodiimide cross-linking between nanospheres and produces oligomers exhibiting sub-nanometer gap spacing over mm 2 areas. Anhydride linkers between nanospheres are observed via surface-enhanced Raman scattering (SERS) spectroscopy. The anhydride linkers are cleavable via nucleophilic substitution and enable placement of nucleophilic molecules in electromagnetic hotspots. Atomistic simulations elucidate that the transient attractive force provided by EHD flow is needed to provide a sufficient residence time for anhydride cross-linking to overcome slow reaction kinetics. This synergistic analysis shows assembly involves an interplay between long-range driving forces increasing nanoparticle− nanoparticle interactions and probability that ligands are in proximity to overcome activation energy barriers associated with short-range chemical reactions. Absorption spectroscopy and electromagnetic full-wave simulations show that variations in nanogap spacing have a greater influence on optical response than variations in close-packed oligomer geometry. The EHD flow−anhydride cross-linking assembly method enables close-packed oligomers with uniform gap spacings that produce uniform SERS enhancement factors. These results demonstrate the efficacy of colloidal driving forces to selectively enable chemical reactions leading to future assembly platforms for large-area nanodevices.

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“…Meanwhile, noble metal NPs modified electrodes have shown promising applications in the areas of catalysis, (electro)chemical analysis and biosensing due to their unique characters such as good conductivity, strong capacity to promote electronic transmission, high catalytic activity and high sensitivity and/or selectivity in electrochemical analysis [4][5][6][7]. For example, Au NPs can promote electrocatalytic reaction by accelerating electron transfer process in redox center.…”

Section: Introductionsmentioning

confidence: 99%

A facile method to prepare noble metal nanoparticles modified Self-Assembly (SAM) electrode

Liu

Huang

Duan

et al. 2017

Journal of Experimental Nanoscience

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Noble metal nanoparticles (NPs) modified electrodes have shown promising applications in the areas of catalysis, (electro)chemical analysis and biosensing due to their unique characters. In this paper, we introduced a so-called ligand exchange method to prepare self-assembly (SAM) electrode modified with noble metal nanoparticles. The noble metal nanoparticles protected by weakly adsorbed tetraoctylammonium bromide (TOAB) were synthesized firstly, then self-assembly (SAM) dithiol-modified Au electrode (Au-SH SAM ) was immersed into the solutions containing TOAB-protected nanoparticles. Due to the strong interaction between the dithiol groups on the electrode and noble metal nanoparticles, the weakly adsorbed TOAB on the surface of noble metal NPs were replaced by dithiol groups. As a result, the TOAB protected NPs were anchored on the Au-SH SAM template electrode surface by ligand exchange, obtaining noble metal NPs modified electrode with high quality and stability. By adjusting the soaking time, the coverage of nanoparticles on the Au-SH SAM electrode surface could be controlled. The morphology and distribution of noble metal NPs on Au-SH SAM surface was analysis by scanning tunneling microscope (STM), and their electrochemical property was studied by cyclic voltammetry (CV) in H 2 SO 4 solution. The approach is proved as a universal way to prepare noble metal NPs modified SAM electrode.

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Functionalization of Thioctic Acid-Capped Gold Nanoparticles for Specific Immobilization of Histidine-Tagged Proteins

Cited by 254 publications

References 59 publications

Applications of Nanoparticles in Biology

Applications of Nanoparticles in Biology

Driving Chemical Reactions in Plasmonic Nanogaps with Electrohydrodynamic Flow

A facile method to prepare noble metal nanoparticles modified Self-Assembly (SAM) electrode

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