Functionalized carbon nanotube adsorption interfaces for electron transfer studies of galactose oxidase

Wayu, Mulugeta B.; Pannell, Michael J.; Labban, Najwa; Case, William S.; Pollock, Julie A.; Leopold, Michael C.

doi:10.1016/j.bioelechem.2018.10.003

Cited by 21 publications

(15 citation statements)

References 49 publications

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“…We will make use of this knowledge and specifically bind biomolecules on native and genetically functionalized S-layer fusion protein coated MWNTs (Figure 7a). CNTs and graphene have already shown great potential in the development of biosensors due to their huge surface area, great electron transfer rate, good electrical conductivity, and ability to immobilize biologically functional molecules, such as enzymes, aptamers, or receptors [12][13][14][15][16][17][18][19]. Concerning S-layers, a considerable amount of knowledge has accumulated concerning the fabrication of amperometric sensors for glucose [64] or sucrose [65] or of optical sensors for glucose too [66].…”

Section: Discussionmentioning

confidence: 99%

“…Proteins bound to the surface of CNTs are preferred in life-sciences because they provide a better biocompatibility and offer functional groups that may either be used for binding additional molecules in biosensor applications [12][13][14][15][16][17][18][19] or enable further chemical modifications, e.g., for the delivery of drugs, DNA and genes [20,21]. Nevertheless, although several proteins, such as bovine serum albumin (BSA), have already been successfully attached to CNTs through various physical or chemical methods, high resolution microscopical studies have demonstrated that their arrangement and density on the CNT surface and consequently the availability of functional groups varies considerably [5].…”

Section: Introductionmentioning

confidence: 99%

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S-Layer Protein Coated Carbon Nanotubes

et al. 2019

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Carbon nanotubes (CNTs) have already been considered for medical applications due to their small diameter and ability to penetrate cells and tissues. However, since CNTs are chemically inert and non-dispersible in water, they have to be chemically functionalized or coated with biomolecules to carry payloads or interact with the environment. Proteins, although often only randomly bound to the CNT surface, are preferred because they provide a better biocompatibility and present functional groups for binding additional molecules. A new approach to functionalize CNTs with a closed and precisely ordered protein layer is offered by bacterial surface layer (S-layer) proteins, which have already attracted much attention in the functionalization of surfaces. We could demonstrate that bacterial S-layer proteins (SbpA of Lysinibacillus sphaericus CCM 2177 and the recombinant fusion protein rSbpA31-1068GG comprising the S-layer protein and two copies of the IgG binding region of Protein G) can be used to disperse and functionalize oxidized multi walled CNTs. Following a simple protocol, a complete surface coverage with a long-range crystalline S-layer lattice can be obtained. When rSbpA31-1068GG was used for coating, the introduced functionality could be confirmed by binding gold labeled antibodies via the IgG binding domain of the fusion protein. Since a great variety of functional S-layer fusion proteins has already been described, our new technology has the potential for a broad spectrum of functionalized CNTs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

S-Layer Protein Coated Carbon Nanotubes

et al. 2019

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“…Functionalities on the carbon surface can alternatively be induced by amine electrooxydation generating a nitrogen-carbon bond [36]. Finally, modified-CNT and NP-based electrodes can be combined, as demonstrated by Minteer and coworkers for laccase and BOD-based O 2 reduction [37], and by Leopold et al for galactose oxidase [38].…”

Section: Chemical Modification Of Metal Nanoparticles (Nps)mentioning

confidence: 99%

Recent advances in surface chemistry of electrodes to promote direct enzymatic bioelectrocatalysis

Mazurenko

Hitaishi

Lojou

2020

Current Opinion in Electrochemistry

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Redox enzymes catalyze major reactions in microorganisms to supply energy for life. Their use in electrochemical biodevices requires their integration on electrodes, while maintaining their activity and optimizing their stability. In return, such applicative development puts forward the knowledge on involved catalytic mechanisms, providing a direct electrode connection of the enzyme is fulfilled. Enzymes being large molecules with active site embedded in an insulating moiety, direct bioelectrocatalysis supposes strategies for specific orientation of the enzyme to be developed. In this review, we summarize recent advances during the past three years in the chemical modification of electrodes favoring direct electrocatalysis. We present the different methodologies used according to the electrode materials, including metals, carbon-based electrodes or porous structures, and discuss the gained insights into bioelectrocatalysis. We especially focus on enzyme engineering which appears as an emerging strategy for enzyme anchoring. Remaining challenges will be discussed with regards to these last findings.

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“…They react with substrate to enable detection and quantification [65]. Indeed, CNT occupancy on the surface of the electrode caused fast electron transfer and decreased the sensitivity of electrochemical detection [77]. Field-effect transistor (FET) based sensors have been introduced and have reported excellent sensing ability [66,78].…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Current Use of Carbon-Based Materials for Biomedical Applications—A Prospective and Review

Rajakumar

Zhang

Gomathi³

et al. 2020

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Among a large number of current biomedical applications in the use of medical devices, carbon-based nanomaterials such as graphene (G), graphene oxides (GO), reduced graphene oxide (rGO), and carbon nanotube (CNT) are frontline materials that are suitable for developing medical devices. Carbon Based Nanomaterials (CBNs) are becoming promising materials due to the existence of both inorganic semiconducting properties and organic π-π stacking characteristics. Hence, it could effectively simultaneously interact with biomolecules and response to the light. By taking advantage of such aspects in a single entity, CBNs could be used for developing biomedical applications in the future. The recent studies in developing carbon-based nanomaterials and its applications in targeting drug delivery, cancer therapy, and biosensors. The development of conjugated and modified carbon-based nanomaterials contributes to positive outcomes in various therapies and achieved emerging challenges in preclinical biomedical applications. Subsequently, diverse biomedical applications of carbon nanotube were also deliberately discussed in the light of various therapeutic advantages.

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Functionalized carbon nanotube adsorption interfaces for electron transfer studies of galactose oxidase

Cited by 21 publications

References 49 publications

S-Layer Protein Coated Carbon Nanotubes

S-Layer Protein Coated Carbon Nanotubes

Recent advances in surface chemistry of electrodes to promote direct enzymatic bioelectrocatalysis

Current Use of Carbon-Based Materials for Biomedical Applications—A Prospective and Review

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