Functionalized Carbon Nanotubes Are Non-Cytotoxic and Preserve the Functionality of Primary Immune Cells

Dumortier, Hélène; Lacotte, Stéphanie; Pastorin, Giorgia; Marega, Riccardo; Wu, Wei; Bonifazi, Davide; Briand, Jean‐Paul; Prato, Maurizio; Muller, Sylviane; Bianco, Alberto

doi:10.1021/nl068003i

Cited by 151 publications

(206 citation statements)

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“…As shown in Supplementary Figure S10, we did not observe any acute toxicity on both K562 and HeLa cells after 3 and 6 days of treatment with concentrations up to 100 µg ml − 1 . These were consistent with other reports 31 . The non-acute toxicity induced by carboxylated SWNTs can be attributed to the removal of metal catalysts from SWNTs after mixed-acid treatment, which has also been verified in our previous studies 32 .…”

Section: Resultssupporting

confidence: 94%

Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

et al. 2012

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Both human telomeric G-rich and C-rich DnA have been considered as specific drug targets for cancer therapy. However, due to i-motif structure instability and lack of specific binding agents, it remains unclear whether stabilization of telomeric i-motif can inhibit telomerase activity. single-walled carbon nanotubes (sWnTs) have been reported as the first ligand that can selectively stabilize human telomeric i-motif DnA. Here we report that sWnTs can inhibit telomerase activity through stabilization of i-motif structure. The persistence of i-motif and the concomitant G-quadruplex eventually leads to telomere uncapping and displaces telomerebinding proteins from telomere. The dysfunctional telomere triggers DnA damage response and elicits upregulation of p16 and p21 proteins. This is the first example that sWnTs can inhibit telomerase activity and interfere with the telomere functions in cancer cells. These results provide new insights into understanding the biomedical effects of sWnTs and the biological importance of i-motif DnA.

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Section: Resultssupporting

confidence: 94%

Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

et al. 2012

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“…It has been proposed that the type of carbon nanomaterial (i.e., SW-CNT, MW-CNT, and fullerene), method of production (i.e., chemical vapor deposition, arc discharge, and laser ablation), extent of purity (i.e., refined or unrefined), presence of residual transition metal catalysts, functionality of different reactive groups, dimension, and method of administration can influence the health effects observed with these nanomaterials (Lam et al 2004;Muller et al 2005;Dumortier et al 2006;Magrez et al 2006;Tian et al 2006;Sayes et al 2007;Muller et al 2008;Poland et al 2008;Shvedova et al 2008;Tong et al 2009). One novel characteristic, however, that has gained some consideration is the potential role of carbon bond defects and acid functional groups in eliciting inflammatory and fibrotic responses with exposure to MW-CNTs .…”

Section: Discussionmentioning

confidence: 99%

Aerosolization System for Experimental Inhalation Studies of Carbon-Based Nanomaterials

Madl

Teague²,

et al. 2012

Aerosol Science and Technology

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Assessing the human health risks associated with engineered nanomaterials is challenging because of the wide range of plausible exposure scenarios. While exposure to nanomaterials may occur through a number of pathways, inhalation is likely one of the most significant potential routes of exposure in industrial settings. An aerosolization system was developed to administer carbon nanomaterials from a dry bulk medium into airborne particles for delivery into a nose-only inhalation system. Utilization of a cannula-based feed system, diamond-coated wheel, aerosolization chamber, and krypton-85 source allows for delivery of otherwise difficult to produce respirable-sized particles. The particle size distribution (aerodynamic and actual) and morphology were characterized for different aerosolized carbon-based nanomaterials (e.g., single-walled carbon nanotubes and ultrafine carbon black). Airborne particles represented a range of size and morphological characteristics, all of which were agglomerated particles spanning in actual size from the nanosize range (<0.1 µm) to sizes greater than 5 and 10 µm for the particle's largest dimension. At a mass concentration of 1000 µg/m 3 , the size distribution as measured by the inertial impactor ranged from 1.3 to 1.7 µm with a σ g between 1.2 and 1.4 for all nanomaterial types. Because the aerodynamic size distribution is similar across different particle types, this system offers an opportunity to explore mechanisms by which different nanomaterial physicochemical characteristics impart different health effects while theoretically maintaining comparable deposition patterns in the lungs. This sys-

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“…Moreover, it has been discovered that CNTs could be metabolized by neutrophil myeloperoxidase [6] and the functionalized CNTs (f-CNTs) have less cytoxicity [7]. In the past 10 years, a variety of f-CNTs including oxidized, aminated, surfactant-assisted dispersed, polymer-assisted dispersed, and bio-molecular modified CNTs have been discovered [8] and utilized as the effective bio-molecular carriers to deliver peptides, proteins, nucleic acids and small molecular drugs into cellular systems.…”

Section: Introductionmentioning

confidence: 99%

Folate and iron difunctionalized multiwall carbon nanotubes as dual-targeted drug nanocarrier to cancer cells

Zhao

et al. 2011

Carbon

138

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Functionalized Carbon Nanotubes Are Non-Cytotoxic and Preserve the Functionality of Primary Immune Cells

Cited by 151 publications

References 0 publications

Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

Insights into the biomedical effects of carboxylated single-wall carbon nanotubes on telomerase and telomeres

Aerosolization System for Experimental Inhalation Studies of Carbon-Based Nanomaterials

Folate and iron difunctionalized multiwall carbon nanotubes as dual-targeted drug nanocarrier to cancer cells

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