Functionalized Carbon Nanotubes Are Non-Cytotoxic and Preserve the Functionality of Primary Immune Cells

Dumortier, Hélène; Lacotte, Stéphanie; Pastorin, Giorgia; Marega, Riccardo; Wu, Wei; Bonifazi, Davide; Briand, Jean‐Paul; Prato, Maurizio; Muller, Sylviane; Bianco, Alberto

doi:10.1021/nl061160x

Cited by 641 publications

(332 citation statements)

References 47 publications

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“…Apart from helping better solubility, nanotube dendrimer composite can be used for DNA and siRNA delivery with better efficiency. Pristine nanotube has also been shown to be toxic compared to chemically functionalized CNTs [47][48][49][50][51] . There are speculation that dendrimer wrapped nanotube can be better suited for the delivery of nucleic acid with less toxicity 41,52 .…”

Section: Introductionmentioning

confidence: 99%

Structure of a carbon nanotube–dendrimer composite

et al. 2013

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Using all atomistic molecular dynamics (MD) simulations we report the microscopic picture of the nanotube-dendrimer complex for PAMAM dendrimer of generation 2 to 4 and carbon nanotube of chirality (6,5). We find compact wrapping conformations of dendrimer onto the nanotube surface for all the three generations of PAMAM dendrimer. The degree of wrapping is more for non-protonated dendrimer compared to the protonated dendrimer. For comparison we also study the interaction of another dendrimer, poly(propyl ether imine) (PETIM), with nanotube and show that PAMAM dendrimer interacts strongly as compared to PETIM dendrimer as is evident from the distance of closest approach as well as the number of close contacts between the nanotube and dendrimer. We also calculate the binding energy between the nanotube and the dendrimer using MM/PBSA methods and attribute the strong binding to the charge transfer between them. Dendrimer wrapping on CNT will make it soluble and can act as an efficient dispersing agent for nanotube.

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Section: Introductionmentioning

confidence: 99%

Structure of a carbon nanotube–dendrimer composite

et al. 2013

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“…A comparison of the results obtained with the two compounds showed that the SKBR3 cells had a delayed response to MWCNT-quin so that their viability only began to decline at 5 µg/mL (P < 0.001). The results of the modified-MWCNTs toxicity at various concentrations could be related to various parameters, such as structure, length, purity, surface chemistry, agglomeration of CNTs in the culture medium, and surface functionalization [35][36][37][38][39].…”

Section: Resultsmentioning

confidence: 99%

Synthesis, characterization, and toxicity of multi-walled carbon nanotubes functionalized with 4-hydroxyquinazoline

Tahermansouri¹,

Mirosanloo²,

Keshel³

et al. 2016

Carbon letters

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The attachment of 2-aminobenzamide to carboxylated multi-wall carbon nanotubes (MWCNTs)-COOH was achieved through the formation of amide bonds. Then, the functionalized MWCNTs, MWCNT-amide, were treated by phosphoryl chloride to produce MWCNT-quin. The products were characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, thermogravimetric analysis, derivative thermogravimetric, steady-state fluorescence spectroscopy, and solubility testing. MWCNT-quin showed photo-electronic properties, which is due to the attachment of the 4-hydroxyquinazoline groups to them as proved by steady-state fluorescence spectroscopy. This suggests intramolecular interactions between the tubes and the attached 4-hydroxyquinazoline. The toxicity of the samples was evaluated in human embryonic kidney HEK293 and human breast cancer SKBR3 cell lines, and the viable cell numbers were measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) after the cells were cultured for 24 h. Cellular investigations showed that the modified MWCNTs, particularly MW-CNT-quin, have considerably significant toxic impact on SKBR3 as compared to HEK293 at the concentration of 5 µg/mL.

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“…The lowered toxicity may be due to a decreased tendency of the hydrophilic materials to associate with cell membranes or to decreased redox activity as a result of hydroxylation. Dumortier et al [31] show the nanotube toxicity is also sensitive to surface functionalization, and the degree of water solubility in particular. Functionalizing nanotubes by an oxidation/amidation treatment to introduce negatively charged surface groups produces water soluble CNTs that do not exert toxic effects on immune cells, B and T lymphocytes [31].…”

Section: The Role Of Carbon Surface Chemistrymentioning

confidence: 99%

“…Dumortier et al [31] show the nanotube toxicity is also sensitive to surface functionalization, and the degree of water solubility in particular. Functionalizing nanotubes by an oxidation/amidation treatment to introduce negatively charged surface groups produces water soluble CNTs that do not exert toxic effects on immune cells, B and T lymphocytes [31]. In contrast, the less-soluble formulation provokes secretion of proinflammatory cytokines from macrophages [31].…”

Section: The Role Of Carbon Surface Chemistrymentioning

confidence: 99%

A Window of Opportunity: Designing Carbon Nanomaterials for Environmental Safety and Health

Guo

Liu

Sanchez

et al. 2007

MSF

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Abstract. Carbon nanomaterials are among the best known and most promising products of the nanotechnology movement. Some early studies suggest that fullerenes and nanotubes may pose significant health risks, and this has given rise to an emerging literature on carbon nanotoxicology. This young field has now begun to yield insight into toxicity mechanisms and the specific material features involved in those mechanisms. This paper explores the potential to alter those material features through post-processing or reformulation with the goal of reducing or eliminating carbon nanomaterial health risks. The paper emphasizes the important roles of metal content and bioavailability, carbon surface chemistry, and nanomaterial aggregation state. The nanotechnology movement has been given a unique "window of opportunity" to systematically investigate the toxicity of nanotechnology products and to develop ways to manage health risks before large scale manufacturing becomes widespread.

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Functionalized Carbon Nanotubes Are Non-Cytotoxic and Preserve the Functionality of Primary Immune Cells

Cited by 641 publications

References 47 publications

Structure of a carbon nanotube–dendrimer composite

Structure of a carbon nanotube–dendrimer composite

Synthesis, characterization, and toxicity of multi-walled carbon nanotubes functionalized with 4-hydroxyquinazoline

A Window of Opportunity: Designing Carbon Nanomaterials for Environmental Safety and Health

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