Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery

Pantarotto, Davide; Singh, Ravi; McCarthy, David; Erhardt, Mathieu; Briand, Jean‐Paul; Prato, Maurizio; Kostarelos, Kostas; Bianco, Alberto

doi:10.1002/anie.200460437

Cited by 1,024 publications

(643 citation statements)

References 30 publications

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“…Preliminary experiments comparing FMNTs uptake in trypsin-treated and untreated CCE ES cells support this hypothesis as enzymatic removal of the glycoprotein layer dramatically increased intracellular accumulation of these unique nanocomposite (data not shown). These results support an energy-independent, nano-endocytotic pathway as originally proposed by Pantorotto and co-workers [20,21] and recently confirmed by Kostarelos, et al [31]. However, our findings are Nano Biomed.…”

Section: Potential Mechanismcontrasting

confidence: 57%

“…In most cases, this transfer process involves clathrin-coated pits rather than caveolae or lipid rafts. In contrast, Pantorotto et al hypothesized that the nanotube uptake by living cells can also occur via an energy-independent nanoendocytosis pathway that involves insertion and diffusion of nanotubes across cell membranes [20,21]. Stem cells membranes, however, exhibit a significantly different glycoprotein composition than more mature hematopoietic cells [32].…”

Section: Potential Mechanismmentioning

confidence: 99%

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Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

Cui¹,

Zhang²,

Sheng³

et al. 2010

Nano BioMed ENG

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Stem cells nanotechnology has emerged as a new exciting area, and holds great potential for research and development of stem cells as novel therapeutic platforms for genetic, traumatic, and degenerative medicine. Vital to the success of this technology are approaches that reproducibly facilitate in vivo cell tracking, expansion, differentiation, and transplantation. Herein we reported the effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes (FMNTs) on mice embryonic stem cell line CCE cells. The FMNTs were prepared by plasma surface treatment and characterized by high resolution transmission electron microscopy (HR-TEM), and incubated with murine ES CCE cells for 1 to 28 day.These ES cells were observed by confocal laser scanning microscopy, and were analyzed by real time reverse transcription-polymerase chain reaction (RT-

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Section: Potential Mechanismcontrasting

confidence: 57%

Section: Potential Mechanismmentioning

confidence: 99%

Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

Cui¹,

Zhang²,

Sheng³

et al. 2010

Nano BioMed ENG

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“…As has been reported for fullerenes and CNT, functionalization and the ensuing improvements in the aqueous solubility, and, therefore, biocompatibility of these materials, improve dramatically the toxicity profile observed in vitro (27,28). Previously we reported that the water-soluble f-CNT used in the present study also exhibited a favorable in vitro toxicity profile (16,18). However, no systematic in vivo study has been reported on the toxicological profile of f-CNT.…”

mentioning

confidence: 80%

“…More recently, we reported the first case of CNT-mediated intracellular delivery of plasmid DNA using ammonium-functionalized single-walled CNT (SWNT-NH 3 ϩ ) leading to gene expression levels up to 10-fold that of naked DNA alone (18). These observations pave the way for the use of CNT as delivery systems for therapeutic and diagnostic molecules.…”

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confidence: 88%

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Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers

Singh

Pantarotto

Lacerda

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Carbon nanotubes (CNT) are intensively being developed for biomedical applications including drug and gene delivery. Although all possible clinical applications will require compatibility of CNT with the biological milieu, their in vivo capabilities and limitations have not yet been explored. In this work, water-soluble, singlewalled CNT (SWNT) have been functionalized with the chelating molecule diethylentriaminepentaacetic (DTPA) and labeled with indium ( 111 In) for imaging purposes. Intravenous (i.v.) administration of these functionalized SWNT (f-SWNT) followed by radioactivity tracing using gamma scintigraphy indicated that f-SWNT are not retained in any of the reticuloendothelial system organs (liver or spleen) and are rapidly cleared from systemic blood circulation through the renal excretion route. The observed rapid blood clearance and half-life (3 h) of f-SWNT has major implications for all potential clinical uses of CNT. Moreover, urine excretion studies using both f-SWNT and functionalized multiwalled CNT followed by electron microscopy analysis of urine samples revealed that both types of nanotubes were excreted as intact nanotubes. This work describes the pharmacokinetic parameters of i.v. administered functionalized CNT relevant for various therapeutic and diagnostic applications.nanomedicine ͉ blood circulation half-life ͉ drug delivery ͉ pharmacokinetics ͉ nanotoxicology

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Nanomaterials for Medical Applications

Kumar

2007

Kirk-Othmer Encyclopedia of Chemical Technology

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Nanomaterials are anticipated to revolutionize disease detection and treatment leading to the realization of a potential world market of medical nanotechnology estimated to be > $3 billion within the next 5 years. This article provides an up to date summary of the current research investigations on Nanomaterials for medical applications. Nanomaterials have been categorized based on their shape, and within each shape they have been further categorized based on their chemical composition. Similarly, the medical applications have been categorized into four main sections:medical diagnosis, medical treatment, medical devices, and tissue engineering. Prior to the discussion of medical applications, approaches to biofunctionalization of nanomaterials are also presented as it is very important that the nanomaterials are compatible in biological environment for their application in medicine.

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Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery

Cited by 1,024 publications

References 30 publications

Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

Effects of CdSe/ZnS quantum dots covered multi-walled carbon nanotubes on murine embryonicstem cells

Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers

Nanomaterials for Medical Applications

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