Functionalized nanoparticles based solid-phase membrane micro-tip extraction and high-performance liquid chromatography analyses of vitamin B complex in human plasma

Abstract

Iron nanoparticles were prepared by a green method following functionalization using 1-butyl-3-methylimidazolium bromide. 1-Butyl-3-methylimidazole iron nanoparticles were characterized using FTIR spectroscopy, energy dispersive X-ray fluorescence, X-ray diffraction, scanning electron microscopy and transmission electron microscopy. The nanoparticles were used in solid-phase membrane micro-tip extraction to separate vitamin B complex from plasma before high-performance liquid chromatography. The optimum conditions obtained were sorbent (15 mg), agitation time (30 min), pH (9.0), desorbing solvent [water (5 mL) + methanol (5 mL) + sodium hydroxide (0.1 N) + acetic acid (d = 1.05 kg/L, pH 5.5), desorbing volume (10 mL) and desorption time (30 min). The percentage recoveries of all the eight vitamin B complex were from 60 to 83%. A high-performance liquid chromatography method was developed using a PhE column (250 × 4.6 mm, 5.0 μm) and water/acetonitrile (95:5, v/v; pH 4.0 with 0.1% formic acid) mobile phase. The flow rate was 1.0 mL/min with detection at 270 and 210 nm. The values of the capacity, separation and resolution factor were 0.57-39.47, 1.12-6.00 and 1.84-26.26, respectively. The developed sample preparation and chromatographic methods were fast, selective, inexpensive, economic and reproducible. The developed method can be applied for analyzing these drugs in biological and environmental matrices.

Application of Composite NanoMaterial to Determine Phenols in Wastewater by Solid Phase Micro Membrane Tip Extraction and Capillary Electrophoresis

“…The nanomaterial, that is, composite iron nanoparticles were manufactured utilizing green knowledge as defined elsewhere [32,33,34,35]. Black tea extract was mixed with ferrous sulphate solution and nanoparticles were prepared by green technology.…”

Silica-coated magnetic iron oxide functionalized with hydrophobic polymeric ionic liquid: a promising nanoscale sorbent for simultaneous extraction of antidiabetic drugs from human plasma prior to their quantitation by HPLC

“…There are several techniques described in the literature for extraction of drugs in biological matrices such as liquid-liquid extraction (LLE), dispersive liquid-liquid microextraction (DLLME), solid phase extraction (SPE), micro-solid-phase extraction (m-SPE), solid-phase membrane micro-tip extraction (SPMMTE), etc. [7][8][9][10] In this case, Saha and coauthors have described probe electrospray ionization mass spectrometry to detection of drugs from plasma samples with no or minimal sample pre-treatment. 11 Recent attempts in sample preparation are particularly focused on improving the quality of analytical results and introducing new technological expansions such as simplication, miniaturization and automation of the whole analytical method.…”