Functionalized polymeric magnetic nanoconstructs for selective capturing and sensitive detection of Salmonella typhimurium

Chattopadhyay, S.; Kaur, Avneet; Jain, Swati; Sabharwal, Prabhjot Kaur; Singh, Harpal

doi:10.1016/j.aca.2016.07.014

Cited by 13 publications

(4 citation statements)

References 42 publications

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“…By covalently linking of specific antibody onto the polymeric shell functionalized MPs, almost 100% of Salmonella in mixed bacterial sample could be captured and the cells concentration was enhanced about 100‐fold. Based on this, the detection limit of IMS‐ELISA could be increased to 10 cells/mL (Chattopadhyay, Kaur, Jain, Sabharwal, & Singh, 2016). The monoclonal antibody was conjugated to the amine groups modified MPs to get IMPs, which were then blocked by PBS containing 0.5% casein to improve specificity.…”

Section: The Application Of Ims In Food Microorganisms Detectionmentioning

confidence: 99%

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Wang

Cai

Gao

et al. 2020

Comp Rev Food Sci Food Safe

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The high efficiency and accurate detection of foodborne pathogens and spoilage microorganisms in food are a task of great social, economic, and public health importance. However, the contamination levels of target bacteria in food samples are very low. Owing to the background interference of food ingredients and negative impact of nontarget flora, the establishment of efficient pretreatment techniques is very crucial for the detection of food microorganisms. With the sig

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Section: The Application Of Ims In Food Microorganisms Detectionmentioning

confidence: 99%

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Wang

Cai

Gao

et al. 2020

Comp Rev Food Sci Food Safe

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“…The physical and chemical characteristics of Fe 3 O 4 nanoparticles may be enhanced by functionalization; making them excellent candidates as catalysts and for biomedicine. To detect Salmonella typhimurium (S. typhimurium), researchers functionalized the Fe 3 O 4 nanoparticles with copolymers to serve as immunomagnetic separators and sensors [36]. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were arrested using polyethyleneimine-modified Fe 3 O 4 @SiO 2 nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Detection of bacterial contaminants via frequency manipulation of amino-groups functionalized Fe₃O₄ nanoparticles based resonant sensor

Kale¹,

Chavan²,

Bhapkar³

et al. 2022

Biomed. Phys. Eng. Express

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Bacterial infections have a large impact on public health. Through this study, we report on the development of complementary split-ring resonators (CSRR) supplemented by functionalized nanoparticles to detect bacteria in the aqueous medium. Iron oxide (Fe3O4) nanoparticles were functionalized with amino groups using (3-aminopropyl) triethoxysilane (APTES) to form (APTES@Fe3O4) nanoparticles, which have a specific affinity towards the bacterial species. This affinity was evaluated using the Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacterial species. The resonant sensor was tuned at 430 MHz and the CSRR sensor bed was further activated using APTES@Fe3O4 nanoparticles. Bacterial detection was studied over a range of concentrations from 2.66 x 109 cells to 2.66 x 108 cells. The sensor actively responded to small changes in bacterial concentration, showing an overall shift in resonance frequency of ~ 44 MHz (~ 40 MHz / cell count) for E. coli and ~ 55 MHz (50.43 MHz / cell count) for S. aureus. Dextran sulphate and Chitosan were used as the references. The magnetic character of the conjugated system exhibited strong interaction of the bacterial species with APTES@Fe3O4, justifying the high selectivity towards these species. This demonstrates the feasibility of a sensitive, fast, portable device, against the traditionally used time-consuming bio-assays.

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“…Van-functionalized MBs have been widely studied for the concentration, detection, and identification of bacteria. 27,30,31 Bovine serum albumin (BSA) is a depot and transport protein in the blood and is widely used as a blocking agent to reduce the nonspecific absorption or incorporation of the target molecule. 32−34 The surface of the BSA protein molecule presents numerous amine groups, which may allow multiple Van molecules to couple to the surface of one BSA protein.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Magnetic separation technology offers the advantage of maintaining bacterial viability while eliminating sample matrix interference. − Magnetic beads (MBs) that have been functionalized with Van can act as highly sensitive, easily manipulated concentration carriers. Van-functionalized MBs have been widely studied for the concentration, detection, and identification of bacteria. ,, Bovine serum albumin (BSA) is a depot and transport protein in the blood and is widely used as a blocking agent to reduce the nonspecific absorption or incorporation of the target molecule. − The surface of the BSA protein molecule presents numerous amine groups, which may allow multiple Van molecules to couple to the surface of one BSA protein. , Given that Van is a wide-spectrum antibiotic that can identify Gram-positive bacteria, Van-BSA-MBs are nonspecific for S. aureus. , Fortunately, based on the report that the surface of S. aureus comprises ample protein A, which can specific combine with the Fc region of mammal IgG.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Detection of Staphylococcus aureus with Vancomycin-Conjugated Magnetic Beads as Enrichment Carriers Combined with Flow Cytometry

Meng

Yang

et al. 2017

ACS Appl. Mater. Interfaces

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A novel sandwich strategy was designed to detect Staphylococcus aureus. The strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. Vancomycin (Van), which exerts a strong antibacterial effect on Gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. To effectively concentrate S. aureus, we used bovine serum albumin (BSA) as the amplification carrier to modify magnetic beads (MBs), which were then functionalized with Van. To improve the specificity of the method for S. aureus detection, we adopted fluorescein isothiocyanate (FITC)-tagged pig immunoglobulin G (FITC-pig IgG) as the signal probe and the second recognition agent that bound between the Fc fragment of pig IgG and protein A in the surface of S. aureus. To quantify S. aureus, we measured the fluorescence signal by flow cytometry (FCM). The use of multivalent magnetic nanoprobes (Van-BSA-MBs) showed a high concentration efficiency (>98%) at bacterial concentrations of only 33 colony-forming units (CFU)/mL. Furthermore, the sandwich mode (FITC-pig IgG/SA/Van-BSA-MBs) also showed ideal specificity because Van and IgG bound with S. aureus at two distinct sites. The detection limit for S. aureus was 3.3 × 10 CFU/mL and the total detection process could be completed within 120 min. Other Gram-positive bacteria and Gram-negative bacteria, including Listeria monocytogenes, Bacillus cereus, Cronobacter sakazakii, Escherichia coli O157:H7, and Salmonella Enteritidis, negligibly interfered with S. aureus detection. The proposed detection strategy for S. aureus possesses attractive characteristics, such as high sensitivity, simple operation, short testing time, and low cost.

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Functionalized polymeric magnetic nanoconstructs for selective capturing and sensitive detection of Salmonella typhimurium

Cited by 13 publications

References 42 publications

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Detection of bacterial contaminants via frequency manipulation of amino-groups functionalized Fe₃O₄ nanoparticles based resonant sensor

Sensitive Detection of Staphylococcus aureus with Vancomycin-Conjugated Magnetic Beads as Enrichment Carriers Combined with Flow Cytometry

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Functionalized polymeric magnetic nanoconstructs for selective capturing and sensitive detection of Salmonella typhimurium

Cited by 13 publications

References 42 publications

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Detection of bacterial contaminants via frequency manipulation of amino-groups functionalized Fe3O4 nanoparticles based resonant sensor

Sensitive Detection of Staphylococcus aureus with Vancomycin-Conjugated Magnetic Beads as Enrichment Carriers Combined with Flow Cytometry

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Detection of bacterial contaminants via frequency manipulation of amino-groups functionalized Fe₃O₄ nanoparticles based resonant sensor