Functionalized Silica-Based Nanoparticles for Photodynamic Therapy

Couleaud, Pierre; Béchet, Denise; Vanderesse, Régis; Barberi‐Heyob, Muriel; Faure, Anne-Charlotte; Roux, Stéphane; Tillement, Olivier; Porhel, Sabine; Guillemin, François; Frochot, Céline

doi:10.2217/nnm.11.31

Cited by 31 publications

(30 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SiO 2 NPs are being proposed as new tools for imaging [23][24][25] and drug and biologic delivery (e.g., nucleic acidbased reagents and siRNA) [26][27][28][29][30]. SiO 2 NPs may exist in many different forms, including different crystallinity, morphology, and pore structure, with each form exhibiting unique characteristics [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Bancos

Tsai

Hackley

et al. 2012

ISRN Nanotechnology

View full text Add to dashboard Cite

Nanoparticles (NPs) are known to interfere with many high-throughput cell viability and cell proliferation assays, which complicates the assessment of their potential toxic effects. The aim of this study was to compare viability and proliferation results for colloidal silica (SiO 2 NP; 7 nm) in the RAW 264.7 mouse macrophage cell line using three different techniques: plate-based assays, flow cytometry analysis, and Coulter counter assays. Our data indicate that CellTiter-Blue, XTT, and CyQuant plate-based assays show increased values over control at low SiO 2 NPs concentrations (0.001-0.01 g/L). SiO 2 NPs show little-to-no interference with flow cytometry and Coulter counter assays, which not only were more reliable in determining cell viability and proliferation at low concentrations in vitro, but also identified changes in cell granularity and size that were not captured by the plate-based assays. At high SiO 2 NP concentrations (1 g/L) all techniques indicated cytotoxicity. In conclusion, flow cytometry and Coulter counter identified new cellular features, and flow cytometry offered more flexibility in analyzing the viability and proliferation profile of SiO 2 NP-treated RAW 264.7 cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Bancos

Tsai

Hackley

et al. 2012

ISRN Nanotechnology

View full text Add to dashboard Cite

show abstract

“…This is consistent with previous studies on this kind of NPs bearing chlorin or porphyrin PSs. 11,25,29 The way by which the PS is added in or on the NP is really crucial because it can have an impact on its photophysical properties and therefore on its photocytotoxic effect. Chu et al designed silica-based NPs encapsulating methylene blue.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, they often display a low tumor tissue selectivity in vivo. 11,12 During the last few decades, one solution emerged using multifunctional nanoparticles (NPs) for PDT, highlighting several advantages. 13 The first one comes from the tumor selectivity.…”

mentioning

confidence: 99%

“…We also previously demonstrated that this effect would play a major role in the destruction of GBM by PDT. 11,29 The goal of the present study was to synthesize an ultrasmall theranostic NP, presenting high affinity for neovessels. In addition to a better PDT efficiency, we can also expect to reach longer intra-tumor retention and detection by MRI of the real tumor volume, taking into account the proliferating part of the tumor tissue.…”

mentioning

confidence: 99%

“…30 The use of a chlorin conjugated to a heptapeptide (ATWLPPR) and their grafting on a NP has already been described for vascular-targeted photodynamic therapy (VTP) and has highlighted very promising in vitro and in vivo results. 11,26,29,[31][32][33] Recently, we evidenced that new peptides have an improved affinity for NRP-1; DKPPR is one of them. 34 This article focuses on the design of a new AGuIX-type nanoplatform for VTP using this DKPPR peptide moiety.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Ultrasmall AGuIX theranostic nanoparticles for vascular-targeted interstitial photodynamic therapy of glioblastoma

Thomas¹,

Colombeau²,

Gries³

et al. 2017

IJN

Self Cite

View full text Add to dashboard Cite

Despite combined treatments, glioblastoma outcome remains poor with frequent local recurrences, indicating that a more efficient and local therapy is needed. In this way, vascular-targeted photodynamic therapy (VTP) could help tumor eradication by destroying its neovessels. In this study, we designed a polysiloxane-based nanoparticle (NP) combining a magnetic resonance imaging (MRI) contrast agent, a photosensitizer (PS) and a new ligand peptide motif (KDKPPR) targeting neuropilin-1 (NRP-1), a receptor overexpressed by angiogenic endothelial cells of the tumor vasculature. This structure achieves the detection of the tumor tissue and its proliferating part by MRI analysis, followed by its treatment by VTP. The photophysical properties of the PS and the peptide affinity for NRP-1 recombinant protein were preserved after the functionalization of NPs. Cellular uptake of NPs by human umbilical vein endothelial cells (HUVEC) was increased twice compared to NPs without the KDKPPR peptide moiety or conjugated with a scramble peptide. NPs induced no cytotoxicity without light exposure but conferred a photocytotoxic effect to cells after photodynamic therapy (PDT). The in vivo selectivity, evaluated using a skinfold chamber model in mice, confirms that the functionalized NPs with KDKPPR peptide moiety were localized in the tumor vessel wall.

show abstract

Photodynamic Therapy in Medicine with Mixed‐Metal/Supramolecular Complexes

Bullock

Holder

2017

Ruthenium Complexes

View full text Add to dashboard Cite

Functionalized Silica-Based Nanoparticles for Photodynamic Therapy

Cited by 31 publications

References 60 publications

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays

Ultrasmall AGuIX theranostic nanoparticles for vascular-targeted interstitial photodynamic therapy of glioblastoma

Photodynamic Therapy in Medicine with Mixed‐Metal/Supramolecular Complexes

Contact Info

Product

Resources

About