Functionally graded electrospun polycaprolactone and β-tricalcium phosphate nanocomposites for tissue engineering applications

Erişken, Cevat; Kalyon, Dilhan M.; Wang, Hongjun

doi:10.1016/j.biomaterials.2008.06.022

Cited by 340 publications

(221 citation statements)

References 46 publications

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“…Therefore, the use of films or membranes represents a promising approach in periodontal treatment to promote tissue regeneration by avoiding migration of epithelial cells into the periodontal pocket and incorporating antibiotics to inhibit the related bacteria growth [13]. Different barrier membranes with a graded functionalised structure are proposed in literature in order to obtain tuned mechanical and degradation characteristics [14,15]. Bottino et al [16] have proposed multilayered membranes consisting of three layers: an inner layer based on poly(DL-lactide-co--caprolactone) (PLCL) and two outer functionalised layers, based on ternary polymeric blends (gelatin, PLCL and PLA) in contact with the surrounding tissues.…”

Section: Introductionmentioning

confidence: 99%

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

et al. 2015

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Article:Gentile, P., Frongia, M.E., Redon, M.C. et al. (4 more authors) (2015) Functionalised nanoscale coatings using Layer-by-Layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces. Acta Biomaterialia.https://doi.org/10.1016/j.actbio.2015.04.009 eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. AbstractIn order to achieve high local biological activity and reduce the risk of side effects of antibiotics in the treatment of periodontal and bone infections, a localised and temporally controlled delivery system is desirable. The aim of this research was to develop a functionalised and resorbable surface to contact soft tissues to improve the antibacterial behaviour during the first week after its implantation in the treatment of periodontal and bone infections. Solvent-cast poly(D,L-lactide-coglycolide acid) (PLGA) films were aminolysed and then modified by Layer-by-Layer technique to obtain a nano-layered coating using poly(sodium4-styrenesulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) as polyelectrolytes. The water-soluble antibiotic, metronidazole (MET), was incorporated from the ninth layer. Infrared spectroscopy showed that the PSS and PAH absorption bands increased with the layer number. The contact angle values had a regular alternate behaviour from the ninth layer. X-Ray Photoelectron Spectroscopy evidenced two distinct peaks, N 1s and S 2p , indicating PAH and PSS had been introduced. Atomic Force Microscopy showed the presence of polyelectrolytes on the surface with a measured roughness about 10 nm after 20 layers deposition.The drug release was monitored by Ultraviolet-visible spectroscopy showing 80% loaded-drug delivery in 14 days. Finally, the biocompatibility was evaluated in vitro with L929 mouse fibroblasts and the antibacterial properties were demonstrated successfully against the keystone periodontal bacteria Porphyromonas gingivalis, which has an influence on implant failure, without compromising in vitro biocompatibility. In this study, PLGA was successfully modified to obtain a localised and temporally controlled drug delivery system, demonstrating the potential value of LbL as a coating technology for the manufacture of me...

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Section: Introductionmentioning

confidence: 99%

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

et al. 2015

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“…A study performed earlier using the extrusion electrospinning process generated a porosity value of 87±2% for PCL-beta-TCP meshes containing a gradient of 35%, by weight, beta-TCP across the mesh. Overall, porosity of our mesh fabricated here seems appropriate for cartilage tissue engineering [22] as well as cartilage-bone interface applications [11].…”

Section: Porosity and Pore Sizementioning

confidence: 91%

“…We and others previously utilized conventional electrospinning to form poly(D,Llactide-co-glycolide) nanofiber meshes with controlled fiber diameters, and evaluated their performance using cells from different sources including tendon fibroblasts [9] and NIH 3T3 fibroblasts [10]. Our group also electrospun PCL and beta tricalcium phosphate (beta-TCP) nanoparticles to generate spatially graded composite meshes for osteochondral (cartilage-bone interface) tissue regeneration applications [11]. Test results confirmed their in vitro cytocompatibility and native-like tissue formation with adipose derived stem cells [12].…”

Section: Introductionmentioning

confidence: 99%

Processing of polycaprolactone and hydroxyapatite to fabricate graded electrospun composites for tendon-bone interface regeneration

Bayrak

Ozcan

Erişken

2016

Journal of Polymer Engineering

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Abstract The process of electrospinning is utilized with different approaches including conventional electrospinning, extrusion electrospinning, and electroblowing to form nanofibrous meshes and composites. Here, we report on the quality and properties of spatially graded polycaprolactone (PCL) and nano-hydroxyapatite (nHA) composite meshes fabricated with multiple-spinneret electrospinning. The composite meshes were characterized in terms of the amount of spatially allocated nHA concentration across the mesh, fiber diameter, porosity, pore size, and hydrophilicity of meshes. Results show that linearly and continuously varying nHA concentration distribution, i.e. graded structure, can be accomplished across the mesh thickness using multiple-spinneret electrospinning, which is in accordance with the change of mineral concentration observed in native tendon-bone interface. Furthermore, incorporation of nanoparticles into nanofibers led to increased fiber diameter as depicted by a shift in fiber diameter distribution, a significant increase in mean fiber diameter from 361±9 nm to 459±21 nm, and an increase in contact angle from 120.01±2.77° to 115.24±1.17°. These findings suggest that the composite meshes formed in this study could serve as model systems to be used as scaffolds in tendon-bone tissue engineering application in particular, and for other tissue-tissue interfaces in a broader context.

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“…The major ECM components contained in these sub-tissues are collagen type II and glycosaminoglycans; collagen type I, glycosaminoglycans, minerals; and collagen type I and minerals, respectively (Table 1). Another school of thought for the hierarchical organization of the cartilage-bone interface is the belief of graded change in the composition of ECM components [6][7][8][9]. Although the compartmental organization approach for cartilage-bone interface components has been dominant for years [5,10], recent studies characterizing cartilagebone, as well as tendon-bone, interface at microscopic dimensions found that mineral composition is changing gradually across the interface [4,11,12].…”

Section: Function Composition and Structurementioning

confidence: 99%

“…However, recent investigations at micrscopic levels showed that the osteochondral interface exhibits a gradual change in the composition of the matrix components [12], leading to a paradigm shift in the understanding of scaffold design. Therefore, more recent investigations focused on the design and fabrication of graded scaffolds for osteochondral interface applications [6,7,19]. In one of these studies, tricalciumphospate (TCP) mineral was embedded in PCL nanofibers to fabricate scaffolds with gradually changing TCP concentrations [6].…”

Section: Therapy Cell Scaffoldmentioning

confidence: 99%

Cartilage-Bone Interface Features, Scaffold and Cell Options for Regeneration

Bayrak¹,

Ozcan²

2016

J Tissue Sci Eng

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Functionally graded electrospun polycaprolactone and β-tricalcium phosphate nanocomposites for tissue engineering applications

Cited by 340 publications

References 46 publications

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces

Processing of polycaprolactone and hydroxyapatite to fabricate graded electrospun composites for tendon-bone interface regeneration

Cartilage-Bone Interface Features, Scaffold and Cell Options for Regeneration

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