Functions Encoded by Pyrrolnitrin Biosynthetic Genes from
            <i>Pseudomonas fluorescens</i>

Kirner, Sabine; Hammer, Philip E.; Hill, D. Steven; Altmann, Annett; Fischer, Ilona; Weislo, L. J.; Lanahan, Mike; Pée, Karl‐Heinz van; Ligón, James M.

doi:10.1128/jb.180.7.1939-1943.1998

Cited by 213 publications

(149 citation statements)

References 27 publications

Supporting

Mentioning

140

Contrasting

Unclassified

Order By: Relevance

“…fluorescens BL915 (GenBank accession number U74493) to amplify a 638-bp segment. The prnD product catalyses the oxidation of the amino group of aminopyrrolnitrin to a nitro group to form pyrrolnitrin (Kirner et al 1998). PltC-F (5¢-TTTCATCCTGGTCAG-CAAG-3¢) and PltC-R (5¢-AGCACATGGGCGTTGGT-3¢) were derived from conserved sequences within the pltC gene from Ps.…”

Section: Detection Of Antibiotic Genes In Pseudomonas Cmr5c and Cmr12mentioning

confidence: 99%

Characterization of CMR5c and CMR12a, novel fluorescentPseudomonasstrains from the cocoyam rhizosphere with biocontrol activity

Perneel

Heyrman

Adiobo

et al. 2007

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aim: To screen for novel antagonistic Pseudomonas strains producing both phenazines and biosurfactants that are as effective as Pseudomonas aeruginosa PNA1 in the biocontrol of cocoyam root rot caused by Pythium myriotylum. Material and Results: Forty pseudomonads were isolated from the rhizosphere of healthy white and red cocoyam plants appearing in natural, heavily infested fields in Cameroon. In vitro tests demonstrated that Py. myriotylum antagonists could be retrieved from the red cocoyam rhizosphere. Except for one isolate, all antagonistic isolates produced phenazines. Results from whole‐cell protein profiling showed that the antagonistic isolates are different from other isolated pseudomonads, while BOX‐PCR revealed high genomic similarity among them. 16S rDNA sequencing of two representative strains within this group of antagonists confirmed their relatively low similarity with validly described Pseudomonas species. These antagonists are thus provisionally labelled as unidentified Pseudomonas strains. Among the antagonists, Pseudomonas CMR5c and CMR12a were selected because of their combined production of phenazines and biosurfactants. For strain CMR5c also, production of pyrrolnitrin and pyoluteorin was demonstrated. Both CMR5c and CMR12a showed excellent in vivo biocontrol activity against Py. myriotylum to a similar level as Ps. aeruginosa PNA1. Conclusion: Pseudomonas CMR5c and CMR12a were identified as novel and promising biocontrol agents of Py. myriotylum on cocoyam, producing an arsenal of antagonistic metabolites. Significance and Impact of the Study: Present study reports the identification of two newly isolated fluorescent Pseudomonas strains that can replace the opportunistic human pathogen Ps. aeruginosa PNA1 in the biocontrol of cocoyam root rot and could be taken into account for the suppression of many plant pathogens.

show abstract

Section: Detection Of Antibiotic Genes In Pseudomonas Cmr5c and Cmr12mentioning

confidence: 99%

Characterization of CMR5c and CMR12a, novel fluorescentPseudomonasstrains from the cocoyam rhizosphere with biocontrol activity

Perneel

Heyrman

Adiobo

et al. 2007

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…The PRN biosynthetic locus has been described in detail for P. fluorescens strain BL915. The locus is organized as an operon comprising the prnABCD genes; no pathway-specific regulator has been described for this locus (Hammer et al 1997;Kirner et al 1998;van Pée and Ligon 2000). Besides these pathway-specific regulators, a number of global regulatory elements are involved in the control of the biosyntheses of DAPG, PLT and PRN, among them the sigma factors RpoD, RpoS and RpoN (Sarniguet et al 1995;Schnider et al 1995;Haas and Keel 2003;Péchy-Tarr et al 2005) and a two-component system composed of the sensor kinase GacS and the response regulator GacA (reviewed by Haas and Keel 2003).…”

Section: Introductionmentioning

confidence: 99%

Use of green fluorescent protein-based reporters to monitor balanced production of antifungal compounds in the biocontrol agentPseudomonas fluorescensCHA0

Baehler

Bottiglieri

Péchy-Tarr

et al. 2005

Journal of Applied Microbiology

108

View full text Add to dashboard Cite

Aims: To develop reporter constructs based on stable and unstable variants of the green fluorescent protein (GFP) for monitoring balanced production of antifungal compounds that are crucial for the capacity of the root-colonizing Pseudomonas fluorescens strain CHA0 to control plant diseases caused by soil-borne pathogenic fungi. Methods and Results: Pseudomonas fluorescens CHA0 produces the three antifungal metabolites 2,4-diacetylphloroglucinol (DAPG), pyoluteorin (PLT) and pyrrolnitrin (PRN). The gfp[mut3] and gfp[AAV] reporter genes were fused to the promoter regions of the DAPG, PLT and PRN biosynthetic genes. The reporter fusions were then used to follow the kinetics of expression of the three antifungal metabolites in a microplate assay. DAPG and PLT were found to display an inverse relationship in which each metabolite activates its own biosynthesis while repressing the synthesis of the other metabolite. PRN appears not to be involved in this balance. However, the microbial and plant phenolic metabolite salicylate was found to interfere with the expression of both DAPG and PLT. Conclusions:The results obtained provide evidence that P. fluorescens CHA0 may keep the antifungal compounds DAPG and PLT at a fine-tuned balance that can be affected by certain microbial and plant phenolics. Significance and Impact of the Study: To our knowledge, the present study is the first to use stable and unstable GFP variants to study antibiotic gene expression in a biocontrol pseudomonad. The developed reporter fusions will be a highly valuable tool to study in situ expression of this bacterial biocontrol trait on plant roots, i.e. at the site of pathogen suppression.

show abstract

“…[7]. The prnD gene product catalyzes the oxidation of the amino group of aminopyrrolnitrin to a nitro group to form PRN [39].…”

Section: Primersmentioning

confidence: 99%

Polymorphisms within the prnD and pltC genes from pyrrolnitrin and pyoluteorin-producing Pseudomonas and Burkholderia spp.

Souza¹,

Raaijmakers²

2003

FEMS Microbiology Ecology

124

View full text Add to dashboard Cite

Pyrrolnitrin (PRN) and pyoluteorin (PLT) are broad-spectrum antibiotics produced by several strains of Pseudomonas and Burkholderia species. Both antibiotics play an important role in the suppression of multiple plant pathogenic fungi. Primers were developed from conserved sequences and amplified prnD and pltC fragments from 18 Pseudomonas and four Burkholderia spp. of worldwide origin that produce either PRN or PLT or both. Subsequent RFLP (restriction fragment length polymorphisms) analysis of the 438-bp pltC fragment showed no polymorphisms among PLT-producing Pseudomonas strains. Polymorphisms within the 786-bp prnD fragment, however, allowed the assessment of the diversity among PRN-producing Pseudomonas and Burkholderia spp. to a level similar to that obtained by three 10-mer primers in random amplified polymorphic DNA analysis. Phylogenetic analysis of 16S rDNA sequences of strains representative of PRN-producing Pseudomonas and Burkholderia species correlated well with their taxonomic status. Phylogenetic relationships inferred from each of the four prn genes and from the complete sequence of the prn biosynthetic locus were similar to 16S rDNA-based phylogeny for most strains, except for Burkholderia pyrrocinia DSM 10685. Both RFLP analysis and comparison of the prn gene sequences showed that B. pyrrocinia DSM 10685 was more closely related to PRN-producing Pseudomonas strains, suggesting that lateral gene transfer may have occurred. Colony hybridization and PCR with PRN- and PLT-specific probes and primers showed that Pseudomonas and Burkholderia spp. harboring the prnD and pltC gene were not present at detectable levels on roots of wheat grown in five agricultural soils collected in The Netherlands, two of them being naturally suppressive to Gaeumannomyces graminis var. tritici. These results suggest that PRN- and PLT-producing Pseudomonas and Burkholderia sp. do not contribute to the natural suppressiveness found in these Dutch take-all decline soils.

show abstract

Functions Encoded by Pyrrolnitrin Biosynthetic Genes from Pseudomonas fluorescens

Cited by 213 publications

References 27 publications

Characterization of CMR5c and CMR12a, novel fluorescentPseudomonasstrains from the cocoyam rhizosphere with biocontrol activity

Characterization of CMR5c and CMR12a, novel fluorescentPseudomonasstrains from the cocoyam rhizosphere with biocontrol activity

Use of green fluorescent protein-based reporters to monitor balanced production of antifungal compounds in the biocontrol agentPseudomonas fluorescensCHA0

Polymorphisms within the prnD and pltC genes from pyrrolnitrin and pyoluteorin-producing Pseudomonas and Burkholderia spp.

Contact Info

Product

Resources

About