Functions of animal microRNA‐encoded peptides: the race is on!

Dozier, Christine; Plaza, Serge

doi:10.15252/embr.202254789

Cited by 10 publications

(5 citation statements)

References 11 publications

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“…These ORFs can encode putative miRNA-encoded peptides (miPEPs) [6]. MiPEPs were further identified in animals [7]. Here, we highlight the most important published findings regarding miPEPs, from their discovery to their biological functions in both plants and animals, and we present the most recent data regarding the molecular mechanisms underlying miPEP activity.…”

Section: Introductionmentioning

confidence: 93%

The Essentials on microRNA-Encoded Peptides from Plants to Animals

et al. 2023

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Primary transcripts of microRNAs (pri-miRNAs) were initially defined as long non-coding RNAs that host miRNAs further processed by the microRNA processor complex. A few years ago, however, it was discovered in plants that pri-miRNAs actually contain functional open reading frames (sORFs) that translate into small peptides called miPEPs, for microRNA-encoded peptides. Initially detected in Arabidopsis thaliana and Medicago truncatula, recent studies have revealed the presence of miPEPs in other pri-miRNAs as well as in other species ranging from various plant species to animals. This suggests that miPEP numbers remain largely underestimated and that they could be a common signature of pri-miRNAs. Here we present the most recent advances in miPEPs research and discuss how their discovery has broadened our vision of the regulation of gene expression by miRNAs, and how miPEPs could be interesting tools in sustainable agriculture or the treatment of certain human diseases.

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Section: Introductionmentioning

confidence: 93%

The Essentials on microRNA-Encoded Peptides from Plants to Animals

et al. 2023

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“…Multiple classes and regions of RNA, both coding and noncoding, have been shown to harbor sORFs in prokaryotes and eukaryotes (Figure 1). Functional sORFs have been discovered in small and long noncoding RNAs (ncRNAs and lncRNAs) [32,33], antisense lncRNAs [34,35], microRNA (miRNA) precursors [36], and circular RNAs [37] in bacteria, plants, and other eukaryotes. Interestingly, an increasing number of genes have been shown to exert functions both at the level of the RNA and of the encoded microprotein, such as sgrST [38], azuCR [39], Spot42/SpfP [40], and some miRNAs [36,41].…”

Section: F I G U R Ementioning

confidence: 99%

“…Functional sORFs have been discovered in small and long noncoding RNAs (ncRNAs and lncRNAs) [32,33], antisense lncRNAs [34,35], microRNA (miRNA) precursors [36], and circular RNAs [37] in bacteria, plants, and other eukaryotes. Interestingly, an increasing number of genes have been shown to exert functions both at the level of the RNA and of the encoded microprotein, such as sgrST [38], azuCR [39], Spot42/SpfP [40], and some miRNAs [36,41]. Additional classes of sORFs have been identified in multicistronic mRNAs alongside canonical protein coding sequences (CDSs) in both prokaryotes and eukaryotes.…”

Section: F I G U R Ementioning

confidence: 99%

Microproteins—Discovery, structure, and function

Mohsen,

Martel,

Slavoff

2023

Proteomics

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Advances in proteogenomic technologies have revealed hundreds to thousands of translated small open reading frames (sORFs) that encode microproteins in genomes across evolutionary space. While many microproteins have now been shown to play critical roles in biology and human disease, a majority of recently identified microproteins have little or no experimental evidence regarding their functionality. Computational tools have some limitations for analysis of short, poorly conserved microprotein sequences, so additional approaches are needed to determine the role of each member of this recently discovered polypeptide class. A currently underexplored avenue in the study of microproteins is structure prediction and determination, which delivers a depth of functional information. In this review, we provide a brief overview of microprotein discovery methods, then examine examples of microprotein structures (and, conversely, intrinsic disorder) that have been experimentally determined using crystallography, cryo‐electron microscopy, and NMR, which provide insight into their molecular functions and mechanisms. Additionally, we discuss examples of predicted microprotein structures that have provided insight or context regarding their function. Analysis of microprotein structure at the angstrom level, and confirmation of predicted structures, therefore, has potential to identify translated microproteins that are of biological importance and to provide molecular mechanism for their in vivo roles.

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“…However, recent studies have shown that primary miRNAs (pri-miRNAs) can encode for regulatory peptides, called miRNA-encoded peptides (miPEPs), which specifically enhance the levels of mature miRNAs by increasing the transcription of corresponding pri-miRNA (Lauressergues et al, 2022). So far, miPEPs have been reported in plant species and even in animals (Dozier and Plaza, 2022). The miPEPs are proved to be a powerful agronomic tool without dealing with genomic manipulations (Yadav et al, 2021a).…”

Section: Roles Of Mirna Encoded Peptide In Plantsmentioning

confidence: 99%

Roles of microRNAs in abiotic stress response and characteristics regulation of plant

Zhang

Yang²,

Zhang³

et al. 2022

Front. Plant Sci.

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MicroRNAs (miRNAs) are a class of non-coding endogenous small RNAs (long 20–24 nucleotides) that negatively regulate eukaryotes gene expression at post-transcriptional level via cleavage or/and translational inhibition of targeting mRNA. Based on the diverse roles of miRNA in regulating eukaryotes gene expression, research on the identification of miRNA target genes has been carried out, and a growing body of research has demonstrated that miRNAs act on target genes and are involved in various biological functions of plants. It has an important influence on plant growth and development, morphogenesis, and stress response. Recent case studies indicate that miRNA-mediated regulation pattern may improve agronomic properties and confer abiotic stress resistance of plants, so as to ensure sustainable agricultural production. In this regard, we focus on the recent updates on miRNAs and their targets involved in responding to abiotic stress including low temperature, high temperature, drought, soil salinity, and heavy metals, as well as plant-growing development. In particular, this review highlights the diverse functions of miRNAs on achieving the desirable agronomic traits in important crops. Herein, the main research strategies of miRNAs involved in abiotic stress resistance and crop traits improvement were summarized. Furthermore, the miRNA-related challenges and future perspectives of plants have been discussed. miRNA-based research lays the foundation for exploring miRNA regulatory mechanism, which aims to provide insights into a potential form of crop improvement and stress resistance breeding.

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Functions of animal microRNA‐encoded peptides: the race is on!

Cited by 10 publications

References 11 publications

The Essentials on microRNA-Encoded Peptides from Plants to Animals

The Essentials on microRNA-Encoded Peptides from Plants to Animals

Microproteins—Discovery, structure, and function

Roles of microRNAs in abiotic stress response and characteristics regulation of plant

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