Functions of Chloroplastic Adenylate Kinases in Arabidopsis

Lange, Peter; Geserick, Claudia; Tischendorf, Gilbert; Zrenner, Rita

doi:10.1104/pp.107.114702

Cited by 51 publications

(59 citation statements)

References 69 publications

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“…The chloroplastic adenylate kinase is thought to play a vital role in the equilibration of adenylates and de novo synthesis of ADP in chloroplast, and the absence of AMK2 causes loss of chloroplast integrity [34]. The upregulation of chloroplastic adenylate kinase (spot 73) in this study may provide more adenylate substrate for ATP synthesis in stressed chloroplasts.…”

Section: Discussionmentioning

confidence: 77%

Short-Term Chromium-Stress-Induced Alterations in the Maize Leaf Proteome

Wang

Gao

Guo

et al. 2013

IJMS

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Soil contamination by chromium (Cr) has become an increasing problem worldwide as a result of extensive industrial activities. Chromium, especially hexavalent Cr, impairs the growth and productivity of plants. Although it has been proposed that plants could modify their metabolism to adapt to Cr stress by reprogramming the expression of genes, especially those related to the antioxidant system, damage response, and electron transport chain, evidence at the protein expression level is lacking. To better understand the precise mechanisms underlying Cr phytoxicity and the plant response to Cr exposure, the time-course of changes in the protein expression profile induced by short-term hexavalent Cr exposure (1, 6 and 24 h) were analyzed in maize leaves. Among the over 1200 protein spots detected reproducibly by two-dimensional electrophoresis (2-DE), 60 were found to be differentially accumulated during Cr stress treatment. Of the Cr-regulated proteins, 58 were identified using tandem mass spectrometry (MS/MS). The Cr-regulated proteins identified were mainly involved in ROS detoxification and defense responses (26%), photosynthesis and chloroplast organization (22%), post-transcriptional processing of mRNA and rRNA (12%), protein synthesis and folding (10%), the DNA damage response (5%), and the cytoskeleton (3%). The possible involvement of these Cr stress-responsive proteins in Cr phytoxicity and the plant response to Cr exposure in maize is discussed, taking into consideration the information available from other plant models. Our results provide preliminary evidence that will facilitate understanding the molecular mechanisms underlying Cr toxicity in maize.

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Section: Discussionmentioning

confidence: 77%

Short-Term Chromium-Stress-Induced Alterations in the Maize Leaf Proteome

Wang

Gao

Guo

et al. 2013

IJMS

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“…In this study, such marker reflecting ATP requirement in the energy homeostasis system was the up-regulation of a variety of energy carrier proteins such as 91.4-fold ATPUMP5 (c19814.graph_c0), 9-fold ATP/GTP-binding protein (emb1579, c38770.graph_c0), and 32.2-fold ATBT1 (c47416.graph_c0) during CA (Supplementary Table S3 in Supplementary Material), which were involved in energy transfer. As anonther important envet of maintaining energy balance, adenylate kianase (ADK, c49278.graph_c1) plays a crucial role in regulating the right equilibrium between ADP and ATP levels (Lange et al, 2008), which presented 2.6-fold up-regulation in CA treated seedlings. Moreover, ATP can be an important signaling molecule and generate the proton-motive force to push the Ca 2+ influx across the plasma membrane (Kim et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Insights from the Cold Transcriptome and Metabolome of Dendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation

Chen

et al. 2016

Front. Plant Sci.

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Plant cold acclimation (CA) is a genetically complex phenomenon involving gene regulation and expression. Little is known about the cascading pattern of gene regulatroy network and the link between genes and metabolites during CA. Dendrobium officinale (DOKM) is an important medicinal and ornamental plant and hypersensitive to low temperature. Here, we used the large scale metabolomic and transcriptomic technologies to reveal the response to CA in DOKM seedlings based on the physiological profile analyses. Lowering temperature from 4 to –2°C resulted in significant increase (P < 0.01) in antioxidant activities and electrolyte leakage (EL) during 24 h. The fitness CA piont of 0°C and control (20°C) during 20 h were firstly obtained according to physiological analyses. Subsequently, massive transcriptome and metabolome reprogramming occurred during CA. The gene to metabolite network demonstrated that the CA associated processes are highly energy demanding through activating hydrolysis of sugars, amino acids catabolism and citrate cycle. The expression levels of 2,767 genes were significantly affected by CA, including 153-fold upregulation of CBF transcription factor, 56-fold upregulation of MAPKKK16 protein kinase. Moreover, the gene interaction and regulation network analysis revealed that the CA as an active process, was regulated at the transcriptional, post-transcriptional, translational and post-translational levels. Our findings highligted a comprehensive regulatory mechanism including cold signal transduction, transcriptional regulation, and gene expression, which contributes a deeper understanding of the highly complex regulatory program during CA in DOKM. Some marker genes identified in DOKM seedlings will allow us to understand the role of each individual during CA by further functional analyses.

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“…They catalyze the interconversion of AMP, ADP, and ATP (AMP + ATP↔2 ADP) , and thus play a major role in energetic metabolism through the balance of adenylates . In Plantae, ADKs are found in all plant tissues and in most of the cellular compartments, including chloroplasts and mitochondria . ADKs also contribute to the optimization of ATP production in both chloroplasts and mitochondria through their action on the Mg 2+ equilibrium and membrane potential .…”

Section: Introductionmentioning

confidence: 99%