Functions of ion transport peptide and ion transport peptide-like in the red flour beetle Tribolium castaneum

Begum, Khurshida; Li, Bin; Beeman, Richard W.; Park, Yoonseong

doi:10.1016/j.ibmb.2009.08.005

Cited by 96 publications

(100 citation statements)

References 27 publications

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“…Canton S was used as a wild-type partner in the mating pairs of behavioral tests. Systemic RNAi in the T. castaneum GA1 strain was performed as previously described (8,9,42,43). Two different stages, approximately the fourth to fifth larval stage and early pupal stage, were used for injections of dsRNA.…”

Section: Methodsmentioning

confidence: 99%

“…Drosophila melanogaster has been the best model system, allowing functional studies of neuropeptides and their receptors by the use of highly advanced molecular genetic tools and various publicly available resources (6). A number of other insect species, especially those with sequenced genomes, such as Bombyx mori and Tribolium castaneum, also have been used for investigations into the functions of neuropeptide signals, using piggyBac transformation (7) and RNAi (8,9).Previous studies on insect neuropeptides and their G proteincoupled receptors (GPCRs) have described tachykinin-related peptides (TRPs) and two GPCRs as the receptors for the TRPs in D. melanogaster and other insect species (10-14). In vertebrates tachykinin and the TRPs form a group of ancestral neuropeptides that are found in a wide range of animals, from octopus to human (14-18).…”

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confidence: 99%

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confidence: 99%

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Natalisin, a tachykinin-like signaling system, regulates sexual activity and fecundity in insects

Jiang

Lkhagva

Daubnerová

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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126

166

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An arthropod-specific peptidergic system, the neuropeptide designated here as natalisin and its receptor, was identified and investigated in three holometabolous insect species: Drosophila melanogaster, Tribolium castaneum, and Bombyx mori. In all three species, natalisin expression was observed in 3-4 pairs of the brain neurons: the anterior dorso-lateral interneurons, inferior contralateral interneurons, and small pars intercerebralis neurons. In B. mori, natalisin also was expressed in two additional pairs of contralateral interneurons in the subesophageal ganglion. Natalisin-RNAi and the activation or silencing of the neural activities in the natalisin-specific cells in D. melanogaster induced significant defects in the mating behaviors of both males and females. Knockdown of natalisin expression in T. castaneum resulted in significant reduction in the fecundity. The similarity of the natalisin C-terminal motifs to those of vertebrate tachykinins and of tachykinin-related peptides in arthropods led us to identify the natalisin receptor. A G protein-coupled receptor, previously known as tachykinin receptor 86C (also known as the neurokinin K receptor of D. melanogaster), now has been recognized as a bona fide natalisin receptor. Taken together, the taxonomic distribution pattern of the natalisin gene and the phylogeny of the receptor suggest that natalisin is an ancestral sibling of tachykinin that evolved only in the arthropod lineage.N europeptides are ancestral signaling molecules that function as cell-cell communication mediators in multicellular organisms. Large numbers of diverse neuropeptides are involved in the control of animal behavior, development, and physiology. Recent genomic approaches have revealed diverse groups of neuropeptides in different taxa, based on similarities in the amino acid sequences to neuropeptides discovered in earlier physiological and anatomical studies (1-4). Sequenced genomes of many insect species (5) provide an opportunity to explore the evolutionary processes of neuropeptides and their receptors. Furthermore, the tools available in biotechnology that are readily applicable in suitable insect model species have advanced our understanding of the functions of neuropeptides. Drosophila melanogaster has been the best model system, allowing functional studies of neuropeptides and their receptors by the use of highly advanced molecular genetic tools and various publicly available resources (6). A number of other insect species, especially those with sequenced genomes, such as Bombyx mori and Tribolium castaneum, also have been used for investigations into the functions of neuropeptide signals, using piggyBac transformation (7) and RNAi (8,9).Previous studies on insect neuropeptides and their G proteincoupled receptors (GPCRs) have described tachykinin-related peptides (TRPs) and two GPCRs as the receptors for the TRPs in D. melanogaster and other insect species (10-14). In vertebrates tachykinin and the TRPs form a group of ancestral neuropeptides that are found in a wide rang...

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Natalisin, a tachykinin-like signaling system, regulates sexual activity and fecundity in insects

Jiang

Lkhagva

Daubnerová

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

126

166

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“…Mortality occurring less than 5 days after injection was attributed to injection injury rather than to target transcript knockdown. In general, mortality due to injection damage was <10 % (Begum et al 2009). Pupal injections, which did not cause any detectable defects in adult eclosion, were regarded as parental RNAi experiments (Bucher et al 2002) and were followed by the assessment of oviposition rates and progeny survival.…”

Section: Rna Interferencementioning

confidence: 98%

Identification and characterization of novel ER-based hsp90 gene in the red flour beetle, Tribolium castaneum

Zhang

Liu

et al. 2014

Cell Stress and Chaperones

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Heat-shock protein 90 (HSP90) is a highly conserved molecular chaperone found in all species except for Archaea, which is required not only for stress tolerance but also for normal development. Recently, it was reported that HSP83, one member of the cytosolic HSP90 family, contributes to oogenesis and responds to heat resistance in Tribolium castaneum. Here, a novel ER-based HSP90 gene, Tchsp90, has been identified in T. castaneum. Phylogenetic analysis showed that hsp90s and hsp83s evolved separately from a common ancestor but that hsp90s originated earlier. Quantitative real-time polymerase chain reaction illustrated that Tchsp90 is expressed in all developmental stages and is highly expressed at early pupa and late adult stages. Tchsp90 was upregulated in response to heat stress but not to cold stress. Laval RNAi revealed that Tchsp90 is important for larval/ pupal development. Meanwhile, parental RNAi indicated that it completely inhibited female fecundity and partially inhibited male fertility once Tchsp90 was knocked down and that it will further shorten the lifespan of T. castaneum. These results suggest that Tchsp90 is essential for development, lifespan, and reproduction in T. castaneum in addition to its response to heat stress.

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“…While ITP was initially isolated from CC extracts of locusts [70], it has so far not been reported to occur in CCs of dipterans. Transcripts of ITP in insects are generally found in the brain (4-5 pair of cells), the terminal abdominal ganglion and sometimes in the midgut [71].…”

Section: Diuretic Hormones -Ion Transport Peptidementioning

confidence: 99%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/ pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects.

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Functions of ion transport peptide and ion transport peptide-like in the red flour beetle Tribolium castaneum

Cited by 96 publications

References 27 publications

Natalisin, a tachykinin-like signaling system, regulates sexual activity and fecundity in insects

Natalisin, a tachykinin-like signaling system, regulates sexual activity and fecundity in insects

Identification and characterization of novel ER-based hsp90 gene in the red flour beetle, Tribolium castaneum

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

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