Functions of membrane-bound alcohol dehydrogenase and aldehyde dehydrogenase in the bio-oxidation of alcohols in Gluconobacter oxydans DSM 2003

Wei, Liujing; Jing-lun, Zhou; Zhu, Danni; Cai, Bai-Yi; Lin, Jian; Hua, Qiang; Wei, Dongzhi

doi:10.1007/s12257-012-0339-0

Cited by 19 publications

(18 citation statements)

References 28 publications

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“…4B ). Although the precise location of the biocompatible reaction in E. coli and G. oxydans was not confirmed in either study, primary alcohol oxidation in G. oxydans is known to occur at the cytosolic membrane 42,43 and amino acid importers are known in both microorganisms, suggesting that organocatalysis could occur in the periplasmic space. However, the absence of any enhanced rate in the presence of cells due to substrate/catalyst co-localisation and the high catalyst loading required for product formation in vivo suggests that organocatalysis, in this case, likely occurs in the extracellular media after export of the aldehyde.…”

Section: Biocompatible Chemistry For Small Molecule Synthesismentioning

confidence: 78%

Interfacing non-enzymatic catalysis with living microorganisms

et al. 2021

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Section: Biocompatible Chemistry For Small Molecule Synthesismentioning

confidence: 78%

Interfacing non-enzymatic catalysis with living microorganisms

et al. 2021

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“…Hence, in-depth development and application of biocatalysis for hydroxyl acids production could provide a promising direction for their industrial production. Gluconobacter oxydans (G. oxydans) is a type of Gram-negative bacterium, known for its incomplete oxidation capability, attributed to the presence of membrane-bound dehydrogenases [29,30] such as alcohol [31], aldehyde [32], glycerin [33], and sorbitol dehydrogenases [34], which catalyze alcohols and aldehydes to corresponding acids and ketones. Furthermore, membrane-bound dehydrogenases are directly located on the cell membrane, and substrates are oxidized to products and released into the periplasm without carrier transport, considerably improving the catalytic e ciency [35].…”

Section: Introductionmentioning

confidence: 99%

pH regulatory divergent point for the selective bio-oxidation of primary diols during resting cell catalysis

Hua

Zhang

Han

et al. 2022

Preprint

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Background Hydroxyl acid is an important platform chemical that covers many industrial applications due to dual functional modules. At present, the traditional technology for hydroxyl acid production mainly adopts the petroleum route with benzene, cyclohexane and butadiene and other non-renewable resources as raw materials which violates the law of green chemistry development. Conversely, it is well-known that biotechnology and bioengineering techniques possess several advantages over chemical methods, such as moderate reaction conditions, high chemoselectivity, and are environmental-friendly. However, there still exist some major obstacles for the industrial application of biotechnology as compared with chemical engineering. The critical issue in the competitiveness between bioengineering and chemical engineering is products titer and volume productivity. Therefore, based on the importance of hydroxyl acids in many fields, exploring a clean, environmentally friendly, efficient and practical production technology for the hydroxyl acids preparation is the core purpose of this study. Results To obtain high-purity hydroxyl acid, a microbiological regulation employing Gluconobacter oxydans for its bioproduction was constructed. We achieved a critical point of chain length, determining the end-products. G. oxydans catalyzed diols with chain length ≤ 4, forming hydroxyl acids, and converting 1,5-pentanediol and 1,6-hexanediol to diacids. Based on this principle, we successfully synthesized 75.3 g/L glycolic acid, 83.2 g/L 3-hydroxypropionic acid, and 94.3 g/L 4-hydroxybutyric acid within 48 h. Furthermore, we directionally controlled the products of C5/C6 diols by adjusting pH, resulting in 102.3 g/L 5‑hydroxyvaleric acid and 48.8 g/L 6-hydroxycaproic acid instead of diacids. Combined with pH regulation and cell-recycling, we prepared 271.4 g 5‑hydroxyvaleric acid and 129.4 g 6-hydroxycaproic acid in 6 rounds. Conclusion In this study, a green scheme of employing G. oxydans as biocatalyst for superior-quality hydroxyl acids (C2-C6) production is raised up. The proposed strategy commendably demonstrated a novel technology with simple pH regulation for high-value production of hydroxyl acids via green bioprocess developments.

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“…The genome of G. oxydans DSM2003 and function analysis revealed the presence of oxidoreductases capable of oxidation of alcohols and polyols. A PQQ-dependent alcohol dehydrogenase has been demonstrated its function in the oxidation of primary diols to corresponding hydroxyl aldehydes [17]. However, this enzyme is membrane-bound and difficult to be prepared in large scale; and whole-cell catalysis involved in the further oxidation of glycolaldehyde to glycolic acid due to the presence of aldehyde dehydrogenases in G. oxydans.…”

Section: Introductionmentioning

confidence: 99%