Functions of ocular surface mucins in health and disease

Mantelli, Flavio; Argüeso, Pablo

doi:10.1097/aci.0b013e32830e6b04

Cited by 289 publications

(255 citation statements)

References 46 publications

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“…As the iatrogenic damage in glaucoma is mostly due to preservatives, minimizing their administration is an impelling need. Noecker and colleagues showed that antiglaucoma drugs containing low preservative concentrations were associated with less inflammatory infiltrate in the rabbit conjunctiva (Mantelli & Arg€ ueso 2008). Many clinical studies on humans confirm these laboratory results.…”

Section: Discussionsupporting

confidence: 59%

In vivo confocal microscopy of conjunctiva in preservative‐free timolol 0.1% gel formulation therapy for glaucoma

Frezzotti

Fogagnolo

Haka

et al. 2013

Acta Ophthalmologica

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ABSTRACT.Purpose: To evaluate the effects at 1 year of preservative-free timolol gel and preserved timolol eye drops on conjunctiva and tear parameters. Methods: Forty patients with primary open-angle glaucoma or ocular hypertension were randomized to the two treatment groups and compared with 20 healthy age-matched controls. Clinical tests (IOP, Schirmer I test, and lacrimal film break-up time BUT) and in vivo conjunctival confocal microscopy (IVCM) were performed in all patients at baseline and after 12 months. IVCM (HRT II Rostock Cornea Module; Heidelberg Engineering GmbH, Heidelberg, Germany) was performed after topical anaesthesia in the four cardinal locations and at the corresponding limbus to analyse conjunctiva cells. The main IVCM outcomes were goblet cell density and epithelial regularity. Results: IVCM and clinical parameters were similar in the three groups at baseline. After 12 months, intra-epithelial goblet cell density was significantly lower in the preserved (48.25 AE 7.70) than in the preservative-free beta-blocker group (86.83 AE 22.17, p < 0.001) and controls (88.9 AE 18.33, p < 0.001). The epithelial layer was significantly more regular in the preserved beta-blocker medication group than in the preservative-free beta-blocker group (p < 0.001) and the control group (p < 0.001). A significant reduction in both Schirmer I and BUT was found in the group of preserved timolol (respectively, 11.3 AE 2.97 and 8.12 AE 0.99) compared with preservative-free timolol (16.8 AE 1.83 and 11.27 AE 1.27, p < 0.001) and controls (17.8 AE 1.87 and 12.10 AE 1.28, p < 0.001). Conclusions: Based on our IVCM data, preservative-free beta-blocker gel induces less changes at ocular surface than preserved beta-blockers, a fact that should be considered to obtain less adverse effects and maximal adherence to treatment in a chronic condition such as glaucoma.

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Section: Discussionsupporting

confidence: 59%

In vivo confocal microscopy of conjunctiva in preservative‐free timolol 0.1% gel formulation therapy for glaucoma

Frezzotti

Fogagnolo

Haka

et al. 2013

Acta Ophthalmologica

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“…In addition, MUC5AC mRNA production is up-regulated in response to P. aeruginosa and its exoproducts (Li et al, 1998). The current hypothesis regarding mucin function and tear film structure is that the secreted mucins, such as MUC5AC, are part of the "first-line defence" barrier that prevents pathogens from traversing the mucosal barrier (Davidson and Kuonen 2004;Mantelli and Argüeso 2008). Our study has demonstrated that jellyfish mucins possess potential binding sites for P. aeruginosa and prevent its binding to epithelial cells grown in cell culture.…”

Section: Discussionmentioning

confidence: 93%

Isolation, Biochemical Characterization and Anti-adhesion Property of Mucin from the Blue Blubber Jellyfish (<i>Catostylus mosaicus</i>)

Pearson

Tellam

et al. 2011

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Mucins are large glycoproteins that have been identified as the main component of various biological gels and lubricants. Their compositions contribute to the viscoelastic properties of mucus secretions. Gel-forming mucins have now been identified in a variety of organisms from marine molluscs to humans and are thought to cover and protect epithelial cells from attachment and entry of pathogens. We employed a new approach using a combination of tryptic digestion and the fractionation by hydrophobic interaction chromatography to enable the isolation and purification of mucins from the Blue Blubber jellyfish, Catostylus mosaicus. The purified proteins were stained with Alcian blue indicating extensive glycosylation. Amino acid composition analysis of the purified protein found that it was enriched for Ala, Glu, Thr, Pro and Val residues (together constituting 93 mole % of the protein), which is typical for mucins. Consistent with this, monosaccharide composition analysis revealed extensive O-linked oligosaccharides with N-acetylgalactosamine (GalNAc), galactose (Gal) and N-acetylglucosamine (GlcNAc) as major monosaccharide constituents. The purified C. mosaicus mucins inhibited the attachment of an ocular Pseudomonas aeruginosa (Pa) isolate (Paer6294) to human corneal epithelial (HCE) cells grown in vitro.

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“…Diabetes and other metabolic diseases have also been implicated in the development of cataract. [36][37][38][39] We thus seek to state that, our oculo-visual findings may have possibly been influenced by some conditions present among the butchers and that they are not solely due to the environment in which they work. It was also confirmed that, none of the respondents in this study used any form of personal protective equipment during the singeing of the hide.…”

Section: Discussionmentioning

confidence: 99%

Ocular conditions among singed–hide butchers at The Kumasi abattoir

Kumah

Bisiw

Abdul–Kabir

et al. 2015

JER

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This study attempted to determine the prevalent eye conditions among singed-hide butchers at the Kumasi abattoir (slaughterhouse) in Ghana. A cross-sectional study comprising 80 conveniently chosen singed-hide butchers at the abattoir was conducted. History taken from participants included participants' demographics and ocular and occupational history. Ocular examination included ophthalmoscopy and visual acuity. Diagnoses were made on the presence of a condition(s) in either or both eyes. The Statistical Package for Social Sciences (SPSS) version 17.0 was used to analyze data collected. Descriptive statistics and Fisher's exact test were employed.The overall prevalence of ocular morbidities was 63.8% among the respondents.Ocular itchiness (77.5%) was the most widely reported symptom followed by teary eyes (70.0%). All respondents suffered from one or more oculo-visual symptoms. The commonest ocular diseases were pinguecula (38.8%), followed by pterygium (20.0%), conjunctivitis (2.5%) and cataract (2.5%). It was observed that singed-hide butchers at the Kumasi abattoir were at high risk of developing eye diseases attributable to the working environment.

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Functions of ocular surface mucins in health and disease

Cited by 289 publications

References 46 publications

In vivo confocal microscopy of conjunctiva in preservative‐free timolol 0.1% gel formulation therapy for glaucoma

In vivo confocal microscopy of conjunctiva in preservative‐free timolol 0.1% gel formulation therapy for glaucoma

Isolation, Biochemical Characterization and Anti-adhesion Property of Mucin from the Blue Blubber Jellyfish (<i>Catostylus mosaicus</i>)

Ocular conditions among singed–hide butchers at The Kumasi abattoir

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