Functions of retinal astrocytes and Müller cells in mammalian myopia

Abstract

Background Changes in the retina and choroid blood vessels are regularly observed in myopia. However, if the retinal glial cells, which directly contact blood vessels, play a role in mammalian myopia is unknown. We aimed to explore the potential role and mechanism of retinal glial cells in form deprived myopia. Methods We adapted the mice form-deprivation myopia model by covering the right eye and left the left eye open for control, measured the ocular structure with anterior segment optical coherence tomography, evaluated changes in the morphology and distribution of retinal glial cells by fluorescence staining and western blotting; we also searched the online GEO databases to obtain relative gene lists and confirmed them in the form-deprivation myopia mouse retina at mRNA and protein level. Results Compared with the open eye, the ocular axial length (3.54 ± 0.006 mm v.s. 3.48 ± 0.004 mm, p = 0.027) and vitreous chamber depth (3.07 ± 0.005 mm v.s. 2.98 ± 0.006 mm, p = 0.007) in the covered eye became longer. Both glial fibrillary acidic protein and excitatory amino acid transporters 4 elevated. There were 12 common pathways in human myopia and anoxic astrocytes. The key proteins were also highly relevant to atropine target proteins. In mice, two common pathways were found in myopia and anoxic Müller cells. Seven main genes and four key proteins were significantly changed in the mice form-deprivation myopia retinas. Conclusion Retinal astrocytes and Müller cells were activated in myopia. They may response to stimuli and secretory acting factors, and might be a valid target for atropine.

Sustained Retinal Defocus Increases the Effect of Induced Myopia on the Retinal Astrocyte Template

“…There was also decreased peripheral retinal branchpoint density, along with a central increase in string vessels and retinal branchpoint density [17]. Supporting the existence of retinal glia alterations in myopia, increased GFAP expression and both astrocyte and Müller cell morphology changes have been demonstrated in a mouse model of myopia [18].…”

Identification of miR-671-5p and Its Related Pathways as General Mechanisms of Both Form-Deprivation and Lens-Induced Myopia in Mice

“…Gliogenesis is one of the enriched GO entries ( Figure 3 A), which is indirectly supported by previous studies. It has been reported that the FDM retina tends to have increased numbers of astrocytes and Müller cells [ 52 ]. The secreted serine protease PRSS56 derived from Müller cells was shown to contribute to excessive ocular axial elongation [ 53 ].…”

Characterization of Retinal VIP-Amacrine Cell Development During the Critical Period

“…Western blotting was performed according to our previously reported protocols (Zhang et al 2022 ). In brief, retinas were homogenized in RIPA buffer containing complete protease inhibitor cocktail (Roche).…”

“…qRT-PCR was performed according to our previously reported protocols (Zhang et al 2022 ). Briefly, total RNA was extracted using TRIzol reagent (Takara, Japan).…”