Fundamental design of the high energy undulator pilot beamline for macromolecular crystallography at the SPring-8

Abstract

The SPring-8 project team adopted our proposal for public beamline as one of two pilot beamlines for standardizing the beamline elements. The light source of this beamline is an in-vacuum-type undulator of magnetic periodicity of 3.2 cm, which emits highly brilliant x rays in a wide energy range between 9 and 38 keV. The highest power emitted from the light source is 5 kW. The corresponding power density is 300 kW/mrad2. To handle the tremendous power density, grazing incidence diffraction with variable glancing angle will be used for the first crystal of a fixed-exit double-crystal monochromator. To focus the high energy x rays up to 38 keV, two supermirrors (Ovonic Synthetic Materials Co.) will be installed in vertical and horizontal directions independently to get a quasi-isotropic and small beam profile at a focal position. In the experimental station, the sample crystals will be mounted on the κ-type goniostat, and the Weissenberg photographs will be recorded by on-line mode on an imaging plate detector of high-speed readout. Based on the excellent performance of the beamline, the routine structure determination of macromolecules will become available by using the newly constructed software system corresponding to the multiple isomorphous replacement technique with optimized anomalous scattering. The structure analyses with smaller sample crystals than that usually used and with the specimen of the larger unit cell will also become possible.

Detailed assessment of X-ray induced structural perturbation in a crystalline state protein

“…X-ray data collection and processing for monitoring X-ray damage All diffraction experiments were performed at the beamline BL41XU in SPring-8 (Harima, Japan) (Kamiya et al, 1995(Kamiya et al, , 1998Kawamoto et al, 2001). The storage ring of the synchrotron facility is operated in top-up mode keeping X-ray intensity constant.…”

Asymmetric-cut variable-incident-angle monochromator

“…8 Much theoretical work has been done on this kind of monochromator. [12][13][14][15][16] Some finite element studies were made on this type of monochromator [17][18][19] and some ray tracing calculations were done. 20 The monochromator described in the paper was originally designed as a high heat load monochromator capable of handling 5-10 kW x-ray beams from a wiggler source.…”

Rotated-inclined double-crystal monochromator for synchrotron radiation: aberration and its compensation

“…This approach, based on that of Smither & Fernandez (1994), has been adopted for SPring-8; the geometry was called a rotated-inclined monochromator (see e.g. Kamiya et al, 1995). The purpose of this paper is to show that the results obtained by Busetto & Hrdy  (1995) for the inclined double-crystal monochromator are essentially also valid (with some modi®cations) for the rotated-inclined double-crystal monochromator.…”