Fundamental Study on Bone Formation Using Collagen Orientation Induced by Magnetic Fields

Shinohara, Hajime; Takei, Hikaru; Saito, Daisuke; Kotani, Masahiro; Ueno, Shinji; Nakahira, Atsushi

doi:10.2109/jcersj.114.131

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…The average orientation order parameter F 2D of collagen fiber was 0.91. This value is almost the same value as the result of applying magnetic field strength 8 T by superconducting magnets [5].…”

Section: Resultssupporting

confidence: 72%

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Permanent magnets circuit for collagen orientation

Shinohara¹,

Saito

et al. 2007

Phys. Status Solidi (c)

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To synthesize artificial bones, the collagen orientation is one of the key issues. It is reported that high magnetic field could be generated by superconducting magnets, are effective in controlling collagen orientation. In this paper, magnetic circuit using permanent magnets for collagen orientation is studied. As the results, a compact magnetic circuit with magnetic field strength around 3 T has been developed. By using the magnetic circuit, collagen was highly oriented, and orientation order was 0.9. Mouse osteoblast cells were seeded on the orientated collagen solution and cultured. The collagen-gel shrinkages by cells were observed. The shrinkage rates of oriented collagen are higher than that of not oriented one.

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Section: Resultssupporting

confidence: 72%

“…As the results, it is clarified that magnetic field strength more than 2.5 T is necessary to orient collagen [5].…”

Section: Introductionmentioning

confidence: 77%

Permanent magnets circuit for collagen orientation

Shinohara¹,

Saito

et al. 2007

Phys. Status Solidi (c)

Self Cite

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“…Static MFs, even low MFs [< 1 T (T)], have been reported to accelerate osseous fusion and to promote osteoblastic differentiation at a cellular level ( Aydin and Bezer, 2011 ; Yuge et al, 2003 ; Bruce et al, 1987 ; Yamamoto et al, 2003 ). In addition, static high MFs (≥ 1 T) are reported to regulate the orientation of cells and bone matrix proteins ( Kotani et al, 2000 ; Kotani et al, 2002 ; Shinohara et al, 2006 ). These results suggest that static MFs have the potential to promote the healing of bone with mechanical strength by controlling the orientation of bone matrix.…”

Section: Introductionmentioning

confidence: 99%

Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells

Okada

Yamato²,

Kawakami³

et al. 2021

Bone Reports

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Effects of high magnetic fields [MFs, ≥ 1 T (T)] on osteoblastic differentiation and the orientation of cells or matrix proteins have been reported. However, the effect of low MFs (< 1 T) on the orientation of bone formation is not well known. This study was performed to verify the effects of low MFs on osteoblastic differentiation, bone formation, and orientation of both cells and newly formed bone. An apparatus was prepared with two magnets (190 mT) aligned in parallel to generate a parallel MF. In vitro , bone marrow-derived stromal cells of rats were used to assess the effects of low MFs on cell orientation, osteoblastic differentiation, and mineralization. A bone morphogenetic protein (BMP)-2-induced ectopic bone model was used to elucidate the effect of low MFs on microstructural indices, trabecula orientation, and the apatite c -axis orientation of newly formed bone. Low MFs resulted in an increased ratio of cells oriented perpendicular to the direction of the MF and promoted osteoblastic differentiation in vitro . Moreover, in vivo analysis demonstrated that low MFs promoted bone formation and changed the orientation of trabeculae and apatite crystal in a direction perpendicular to the MF. These changes led to an increase in the mechanical strength of rhBMP-2-induced bone. These results suggest that the application of low MFs has potential to facilitate the regeneration of bone with sufficient mechanical strength by controlling the orientation of newly formed bone.

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Fundamental Study on Bone Formation Using Collagen Orientation Induced by Magnetic Fields

Cited by 2 publications

References 6 publications

Permanent magnets circuit for collagen orientation

Permanent magnets circuit for collagen orientation

Low magnetic field promotes recombinant human BMP-2-induced bone formation and influences orientation of trabeculae and bone marrow-derived stromal cells

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