Fundamentals and Applications of Immunosensors

Moina, Carlos; Ybarra, Gabriel

doi:10.5772/36947

Cited by 40 publications

(29 citation statements)

References 40 publications

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“…Biosensors are compact analytical devices which employ specific biorecognition elements combined with state-of-the-art integrated electronics, and represent an inexpensive alternative to 10 methods which require desktop instrumentation such the enzyme-linked immunosorbent assay (ELISA) [3,4]. Biosensors are characterized by their portability, ease of use and high degree of automation, ideally maintaining high quality analytical standards.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical immunosensor for the determination of β-casein

Molinari

Moina

Ybarra

2015

J. Electrochem. Sci. Eng.

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An amperometric biosensor for the quantification of food allergens based on an inhibitory immunoassay is presented. As a proof of concept, the experimental conditions were optimized for the detection of β-casein in the 0-10 ppm range. Eight electrochemical cells were integrated into a small-sized portable potentiostat controlled by a smartphone via Bluetooth communication. The determination of β-casein in eight different samples can be measured with the electrochemical biosensor, which has the potential to be modified for the detection of multiple allergens.

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Section: Introductionmentioning

confidence: 99%

Electrochemical immunosensor for the determination of β-casein

Molinari

Moina

Ybarra

2015

J. Electrochem. Sci. Eng.

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“…Immunosensors are a kind of biosensor planned to detect the formation of antigenantibody complexes and convert it into electrical signals by means of a suitable transducer [223]. Antibodies are proteins produced by the immune system to recognise bacteria, viruses, parasites and all those microorganisms or macromolecules considered extraneous to the hosting living system.…”

Section: Immunosensorsmentioning

confidence: 99%

Electrospinning for High Performance Sensors

Macagnano¹,

Zampetti²,

Kny³

2015

NanoScience and Technology

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The series NanoScience and Technology is focused on the fascinating nano-world, mesoscopic physics, analysis with atomic resolution, nano and quantum-effect devices, nanomechanics and atomic-scale processes. All the basic aspects and technology-oriented developments in this emerging discipline are covered by comprehensive and timely books. The series constitutes a survey of the relevant special topics, which are presented by leading experts in the field. These books will appeal to researchers, engineers, and advanced students.More information about this series at

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“…Novel methodologies for Brucella detection and identification are still limited since most methods detect the bacteria indirectly using label‐dependent biosensors (Fu‐Chun, De‐Si, Zhong, Shu‐Zhen, & Ya‐Fei, ; Liebes, Amir, Marks, & Banai, ; Wu et al, ) or sensors that require sample pretreatment (Pal et al, ; Rahi, Sattarahmady, & Heli, ). Sensitivity of the immunoassays depends on the immobilization of the antibody on a solid support of sufficient surface density in an orientation that maximizes their antigen capture capability (Holford, Davis, & Higson, ; Moina & Ybarra, ). The use of protein A extracted from Staphylococcus aureus (SpA) has been used to increase the efficiency of recognition site detection of soluble antigens (Tan et al, ; Trilling, Beekwilder, & Zuilhof, ; Yoon, ).…”

Section: Introductionmentioning

confidence: 99%

Detection of Brucella abortus by a platform functionalized with protein A and specific antibodies IgG

Baltierra‐Uribe

Chanona‐Pérez

Méndez-Méndez

et al. 2019

Microscopy Res & Technique

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Oriented immobilization of antibodies on a sensor surface is critical for enhancing both the antigen‐binding capacity and the sensitivity of immunosensors. In this study, we describe a strategy to adsorb immunoglobulin G (IgG) anti‐Brucella antibodies onto a silicon surface, oriented by protein A obtained from Staphylococcus aureus (SpA). X‐ray photoelectron spectroscopy and atomic force microscopy were used to characterize topographically, morphologically, and chemical changes of the sensor functionalization. The activity of the biosensor was assessed by confocal microscopy, scanning electronic microscopy, and bacteria capture assays (BCA). According to the BCA, the efficiency of Brucella abortus detection with the SpA‐IgG anti Brucella biosensor was three‐fold higher than that of the random orientated IgG anti Brucella biosensor. The limit of detection was 1 × 106 CFU/ml. These data show that the orientation of antibodies immobilization is crucial to developing immunosensors for bacterial antigen detection as Brucella spp and improve its sensibility level. Functionalization with protein A increases Brucella detection by an antibody‐coated surface. Functionalized silicon surface for Brucella detection was characterized by atomic force microscopy, X‐ray photoelectron spectroscopy and confocal microscopy.

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Fundamentals and Applications of Immunosensors

Cited by 40 publications

References 40 publications

Electrochemical immunosensor for the determination of β-casein

Electrochemical immunosensor for the determination of β-casein

Electrospinning for High Performance Sensors

Detection of Brucella abortus by a platform functionalized with protein A and specific antibodies IgG

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