Fundamentals of Fermentation Media

Allikian, Kathrine; Edgar, Rebecca; Syed, Rashad; Zhang, Shuguang

doi:10.1007/978-3-030-16230-6_2

Cited by 12 publications

(8 citation statements)

References 127 publications

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“…A well-designed production medium is one of the key factors for successful bacterial fermentation and significantly impacts on the mechanical properties of PHB materials [72]. A report by Oliveira et al investigated the chemical structure, thermal and crystalline properties of PHB samples produced by solid-state fermentation (SSF).…”

Section: Effect Of Medium Compositionmentioning

confidence: 99%

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

et al. 2020

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Plastic pollution is fueling the grave environmental threats currently facing humans, the animal kingdom, and the planet. The pursuit of renewable resourced biodegradable materials commenced in the 1970s with the need for carbon neutral fully sustainable products driving important progress in recent years. The development of bioplastic materials is highlighted as imperative to the solutions to our global environment challenges and to the restoration of the wellbeing of our planet. Bio-based plastics are becoming increasingly sustainable and are expected to substitute fossil-based plastics. Bioplastics currently include both, nondegradable and biodegradable compositions, depending on factors including the origins of production and post-use management and conditions. Among the most promising materials being developed and evaluated is polyhydroxybutyrate (PHB), a microbial bioprocessed polyester belonging to the polyhydroxyalkanoate (PHA) family. This biocompatible and non-toxic polymer is biosynthesized and accumulated by a number of specialized bacterial strains. The favorable mechanical properties and amenability to biodegradation when exposed to certain active biological environments, earmark PHB as a high potential replacement for petrochemical based polymers such as ubiquitous high density polyethylene (HDPE). To date, high production costs, minimal yields, production technology complexities, and difficulties relating to downstream processing are limiting factors for its progression and expansion in the marketplace. This review examines the chemical, mechanical, thermal, and crystalline characteristics of PHB, as well as various fermentation processing factors which influence the properties of PHB materials.

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Section: Effect Of Medium Compositionmentioning

confidence: 99%

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

et al. 2020

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“…Numerous studies have explored and enhanced different aspects of the fermentation process in various contexts, including liquid-state fermentation (LSF) [55,58,[60][61][62][63], solid-state fermentation (SSF) [64][65][66][67][68][69], and biofilm reactors [54,[70][71][72][73][74][75][76], to improve MK-7 production using B. subtilis natto. The fermentation media, in particular, has been of considerable interest, as the selection of carbon, nitrogen, and salt sources and presence of growth factors, vitamins, minerals, bioactives, and other essential nutrients play a crucial role in microbial growth and metabolism [77,78], which ultimately influence the productivity of the fermentation process and the MK-7 yield. Recently, several investigations have focused on improving the MK-7 yield by optimising the fermentation media using DOE and response surface methodology (RSM) [54,61,62,69,79,80].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of the fermentation media to enhance the production of the bioactive isomer of vitamin menaquinone-7

Lal

Seifan

Berenjian

2022

Bioprocess Biosyst Eng

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Menaquinone-7 (MK-7) offers significant health benefits; however, only the all-trans form is biologically active. MK-7 produced through fermentation can occur as all-trans and cis isomers, and the therapeutic value of the resulting MK-7 is exclusively determined by the quantity of the all-trans isomer. Therefore, this study aimed to investigate the effect of the media composition on the isomer profile obtained from fermentation and determine the optimum media combination to increase the concentration of the all-trans isomer and diminish the production of cis MK-7. For this purpose, design of experiments (DOE) was used to screen the most effective nutrients, and a central composite face-centred design (CCF) was employed to optimise the media components. The optimum media consisted of 1% (w/v) glucose, 2% (w/v) yeast extract, 2% (w/v) soy peptone, 2% (w/v) tryptone, and 0.1% (w/v) CaCl2. This composition resulted in an average all-trans and cis isomer concentration of 36.366 mg/L and 1.225 mg/L, respectively. In addition, the optimised media enabled an all-trans isomer concentration 12.2-fold greater and a cis isomer concentration 2.9-fold less than the unoptimised media. This study was the first to consider the development of an optimised fermentation media to enhance the production of the bioactive isomer of MK-7 and minimise the concentration of the inactive isomer. Furthermore, this media is commercially promising, as it will improve the process productivity and reduce the costs associated with the industrial fermentation of the vitamin.

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“…Another study has shown that organic nitrogen sources promote greater mycelial growth as compared to inorganic nitrogen sources. Pigment production was stimulated by meat peptone, casein peptone, the peptone–YE combination, and corn steep powder, but red pigment synthesis was severely hindered by soy peptone and malt extract [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Maximization of red pigment production from Streptomyces sp. LS1 structure elucidation and application as antimicrobial/antifouling against human pathogens and marine microbes

Hemeda

Hegazy

Abdelgalil

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Natural dyes are present in living organisms such as animals and plants and microorganisms such as fungi, bacteria, algae, and yeast. Pigments are fast and easy growth by using cheap components and do not effect by environmental conditions because they required some physical factors like heat, light, and pH and also they have many biotechnological applications such as medical and industrial needs. The natural pigments can act as antimicrobial agents and are used in drug manufacturing. Also, it can be used in the food industry as natural colorants instead of the synthetic colorants due to their safety on human health and low toxicity when emitted into the environment. Results A pigmented actinomycetes LS1 strain isolated from El Mahmoudia canal (sediment soil) located in Egypt was microscopically examined and identified as Streptomyces sp. by molecular approach. Extraction, purification, and characterization of produced red pigment metabolite like carotenoids related were established based on spectroscopic studies and comparing the data from the literature. Factors (nutritional and physical) influencing red pigmentation by this isolate were investigated through One Variable At Time (OVAT), and then, the optimal levels of the significant key variables were recorded. Also, the productivity yield reached 30 mg of dried purified pigment/gram dry weight. The biological activity of the red product was tested against Gram-positive and Gram-negative marine bacterial pathogens; the recorded antimicrobial activity is more prominent against (P. aeruginosa ATCC 9027, K. pneumoniae ATCC 13883, S. aureus ATCC 6538, B. subtilis ATCC 6633 and E. coli ATCC 10418) at nearly 0.07 mg mL−1 concentration. Also, the tested red pigment showed a positive antifouling activity (AF) against marine microbes; the activity increased by increasing the pigment concentrations from 1 to 3 mg mL−1. Conclusion The present work focused on the optimization of culture conditions for the production of red pigment by Streptomyces sp. LS1; then, the antibacterial activity and antifouling activity of the produced pigments were tested.

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Fundamentals of Fermentation Media

Cited by 12 publications

References 127 publications

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

Production of Polyhydroxybutyrate (PHB) and Factors Impacting Its Chemical and Mechanical Characteristics

Optimisation of the fermentation media to enhance the production of the bioactive isomer of vitamin menaquinone-7

Maximization of red pigment production from Streptomyces sp. LS1 structure elucidation and application as antimicrobial/antifouling against human pathogens and marine microbes

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