Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules

Morris, Caleb B.; Poland, James C; May, Jody C.; McLean, John A.

doi:10.1007/978-1-0716-0030-6_1

Cited by 32 publications

(31 citation statements)

References 151 publications

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“…ESI permits online IM analysis of the LC eluates, which is generally impossible with MALDI. The multidimensional separation combined reversed-phase liquid chromatography (RP-LC), which fractionates based on polarity and hydrophilicity/hydrophobicity balance, − with ion mobility spectrometry, which disperses based on charge and collision cross-section (CCS is a parameter describing size and shape, analogous to hydrodynamic volume). ,,− …”

Section: Resultsmentioning

confidence: 99%

Elucidation of Low Molecular Weight Polymers in Vehicular Engine Deposits by Multidimensional Mass Spectrometry

Snyder

Wesdemiotis

2020

Energy Fuels

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Engine oil is mainly comprised of base oils and various additives, which are low molecular weight polymers mixed into the oil resulting in a polymer blend. Dispersants and detergents, the two most abundant additives, are intended to keep the engine free of particulate but are not always successful. At high temperatures, species not combusted may undergo oxidation and degradation or create other byproducts, generating particulate deposition. Knowledge of the molecular makeup of these byproducts is essential, as particulate accumulation can cause serious issues to the engine and ultimately to the operator. In this study, unknown deposits from the air-intake valve of a vehicular engine have been analyzed via a palette of mass spectrometry (MS) methods, including matrix-assisted laser desorption/ionization MS with postacquisition data processing, atmospheric solids analysis probe MS, and electrospray ionization MS interfaced with 2D separation via reversed-phase liquid chromatography and ion mobility spectrometry. Low molecular weight, aminated poly(propylene glycol) and polyisobutylene detergents and a poly(methyl methacrylate) viscosity modifier were conclusively identified in the deposit, along with oxidized polyethylene chains leaked into the oil/additives blend from vehicular tubing and tanks. The use of different methods was essential for the confident elucidation of the low molecular weight macromolecules giving rise to the vehicular engine particulates.

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Section: Resultsmentioning

confidence: 99%

Elucidation of Low Molecular Weight Polymers in Vehicular Engine Deposits by Multidimensional Mass Spectrometry

Snyder

Wesdemiotis

2020

Energy Fuels

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“…Ion mobility mass spectrometry (IM-MS) uses electric fields to drag analytes through a buffer gas, separating the analytes whilst providing structural information, and has had many improvements in recent years [133]. The extra dimension that ion mobility provides increases the peak capacity in LC-MS workflows [134].…”

Section: The Goodmentioning

confidence: 99%

A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

et al. 2020

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Proteomics is the field of study that includes the analysis of proteins, from either a basic science prospective or a clinical one. Proteins can be investigated for their abundance, variety of proteoforms due to post-translational modifications (PTMs), and their stable or transient protein–protein interactions. This can be especially beneficial in the clinical setting when studying proteins involved in different diseases and conditions. Here, we aim to describe a bottom-up proteomics workflow from sample preparation to data analysis, including all of its benefits and pitfalls. We also describe potential improvements in this type of proteomics workflow for the future.

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“…Remember that the DTIMS has a static electric field with a linearly decreasing voltage over the length of the tube. If a DTIMS tube length was increased, the voltage would also increase in order provide enough momentum to push the ions through the drift tube into the detector [11].…”

Section: Twimsmentioning

confidence: 99%

Perspective Chapter: Advantages of Ion Mobility Coupled with HPLC/UPLC

Bussey¹

2022

Analytical Liquid Chromatography - New Perspectives

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Ion mobility is a new separation technique that can be coupled with high performance liquid chromatography (HPLC) or ultra-performance liquid chromatography (UPLC). Variances in cross-sectional ionic areas of different molecules create differential speeds through a gas allowing for millisecond separations. Combining ion mobility with both liquid chromatography and mass spectrometry with fragmentation, separations can be achieved on the second (HPLC), millisecond (ion mobility), and microsecond (mass spectrometry) timescales. This orthogonal separation greatly cleans up mass spectral data of co-eluting peaks from the liquid chromatography and adds to the descriptive data of each ion. With descriptive data such as retention time, cross-sectional area, m/z ratio, and mass spectral fragmentation, many options become available for analytical analysis. Options ranging from descriptive data collation into instrument libraries to sensitivity enhancement for trace analysis will be explored in this chapter along with the description of different forms of ion mobility.

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Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules

Cited by 32 publications

References 151 publications

Elucidation of Low Molecular Weight Polymers in Vehicular Engine Deposits by Multidimensional Mass Spectrometry

Elucidation of Low Molecular Weight Polymers in Vehicular Engine Deposits by Multidimensional Mass Spectrometry

A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of This Field

Perspective Chapter: Advantages of Ion Mobility Coupled with HPLC/UPLC

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