“…[93][94][95] The fast development and commercialization of ENMs and the lack of suitable detection and characterization techniques exacerbated concerns over the potential risk of using ENMs. 44,75,81 These need further clarifications to develop reproducible, adequate and validated methods for the detection and characterization of ENMs for toxicological studies to develop safe nano-based systems for clinical practices. 42,44 In this stage, both in vivo and in vitro are established methods in determining ENMs' toxicity.…”