Fundidesulfovibrio magnetotacticus sp. nov., a sulphate-reducing magnetotactic bacterium, isolated from sediments and freshwater of a pond

Abstract

A sulphate-reducing magnetotactic bacterium, designated strain FSS-1T, was isolated from sediments and freshwater of Suwa Pond located in Hidaka, Saitama, Japan. Strain FSS-1T was a motile, Gram-negative and curved rod-shaped bacterium that synthesizes bullet-shaped magnetite (Fe3O4) nanoparticles in each cell. Strain FSS-1T was able to grow in the range of pH 6.5–8.0 (optimum, pH 7.0), 22–34 °C (optimum, 28 °C) and with 0–8.0 g l−1 NaCl (optimum, 0–2.0 g l−1 NaCl). Strain FSS-1T grew well in the presence of 50 µM ferric quinate as an iron source. The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The major menaquinone was MK-7 (H2). Strain FSS-1T contained desulfoviridin, cytochrome c 3 and catalase, but did not contain oxidase. Strain FSS-1T used fumarate, lactate, pyruvate, malate, formate/acetate, succinate, tartrate, ethanol, 1-propanol, peptone, soytone and yeast extract as electron donors, while the strain used sulphate, thiosulphate and fumarate as electron acceptors. Fumarate was fermented in the absence of electron acceptors. Analysis of the 16S rRNA gene sequence showed that strain FSS-1T is a member of the genus Fundidesulfovibrio . The gene sequence showed 96.7, 95.0, 92.0, 91.2 and 91.4% similarities to the most closely related members of the genera Fundidesulfovibrio putealis B7-43T, Fundidesulfovibrio butyratiphilus BSYT, Desulfolutivibrio sulfoxidireducens DSM 107105T, Desulfolutivibrio sulfodismutans ThAc01T and Solidesulfovibrio magneticus RS-1T, respectively. The DNA G+C content of strain FSS-1T was 67.5 mol%. The average nucleotide identity value between strain FSS-1T and F. putealis B7-43T was 80.7 %. Therefore, strain FSS-1T represents a novel species within the genus Fundidesulfovibrio , for which the name Fundidesulfovibrio magnetotacticus sp. nov. is proposed (=JCM 32405T=DSM 110007T).

A novel strictly anaerobic sulfate-reducing diazotrophic bacterium Fundidesulfovibrio terrae sp. nov., isolated from paddy soil

“…The genus Fundidesulfovibrio proposed by Waite et al , part of the phylum Pseudomonadota , class Deltaproteobacteria , order Desulfovibrionales , family Desulfovibrionaceae , was derived from the reclassification of some species of the genus Desulfovibrio , with Fundidesulfovibrio putealis as the type species [1, 2]. The members of the genus Fundidesulfovibrio are strictly anaerobic, vibrioid or curved-rod-shaped and able to use sulphate, sulphite and thiosulphate, with the production of sulphide and contain the pigment desulfoviridin [3–6]. As of December 2022, three species of the genus Fundidesulfovibrio with validly published names , Fundidesulfovibrio putealis , Fundidesulfovibrio butyratiphilus and Fundidesulfovibrio magnetotacticus , have been described [2].…”

“…As of December 2022, three species of the genus Fundidesulfovibrio with validly published names, Fundidesulfovibrio putealis, Fundidesulfovibrio butyratiphilus and Fundidesulfovibrio magnetotacticus, have been described [2]. Their habitats were distributed in various anaerobic environments, such as an anaerobic municipal sewage sludge digester, deep subsurface aquifer, sediments and freshwater of a pond [4][5][6]. In the present study, during the survey of the diversity of nitrogenfixing bacteria, a novel sulphate-reducing and nitrogen-fixing strain, SG127 T , was obtained from a paddy soil sample.…”