Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using Penicillium species

Moysés, Danuza Nogueira; Teixeira, Danielle Altomari; Waldow, Vinícius de Abreu; Freire, Denise Maria Guimarães; Castro, Aline Machado de

doi:10.1007/s13205-021-02988-1

Cited by 25 publications

(7 citation statements)

References 62 publications

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“…Several microorganisms, including fungi and their unique enzyme systems, are capable of degrading PET (Table 3) [59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76]. Some fungal strains facilitate PET biodegradation into low molecular weight oligomers or monomers such as bis(2-hydroxyethyl)terephthalate (BHET) and mono(2-hydroxyethyl)terephthalate (MHET) [77].…”

Section: Fungal Enzyme-mediated Pet Degradationmentioning

confidence: 99%

“…Some fungal strains facilitate PET biodegradation into low molecular weight oligomers or monomers such as bis(2-hydroxyethyl)terephthalate (BHET) and mono(2-hydroxyethyl)terephthalate (MHET) [77]. The monomeric structural units of PET are linked by ester bonds, which many fungal hydrolytic enzymes can hydrolyze, e.g., esterases, lipases, and cutinases [59][60][61]73]. Esterases cleave the ester bonds (short-chain acyl ester) found in PET monomers and also facilitate the surface modification of the target PET [73].…”

Section: Fungal Enzyme-mediated Pet Degradationmentioning

confidence: 99%

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Fungal Enzymes as Catalytic Tools for Polyethylene Terephthalate (PET) Degradation

Ahmaditabatabaei

Kyazze

Iqbal

et al. 2021

JoF

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The ubiquitous persistence of plastic waste in diverse forms and different environmental matrices is one of the main challenges that modern societies are facing at present. The exponential utilization and recalcitrance of synthetic plastics, including polyethylene terephthalate (PET), results in their extensive accumulation, which is a significant threat to the ecosystem. The growing amount of plastic waste ending up in landfills and oceans is alarming due to its possible adverse effects on biota. Thus, there is an urgent need to mitigate plastic waste to tackle the environmental crisis of plastic pollution. With regards to PET, there is a plethora of literature on the transportation route, ingestion, environmental fate, amount, and the adverse ecological and human health effects. Several studies have described the deployment of various microbial enzymes with much focus on bacterial-enzyme mediated removal and remediation of PET. However, there is a lack of consolidated studies on the exploitation of fungal enzymes for PET degradation. Herein, an effort has been made to cover this literature gap by spotlighting the fungi and their unique enzymes, e.g., esterases, lipases, and cutinases. These fungal enzymes have emerged as candidates for the development of biocatalytic PET degradation processes. The first half of this review is focused on fungal biocatalysts involved in the degradation of PET. The latter half explains three main aspects: (1) catalytic mechanism of PET hydrolysis in the presence of cutinases as a model fungal enzyme, (2) limitations hindering enzymatic PET biodegradation, and (3) strategies for enhancement of enzymatic PET biodegradation.

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Section: Fungal Enzyme-mediated Pet Degradationmentioning

confidence: 99%

Section: Fungal Enzyme-mediated Pet Degradationmentioning

confidence: 99%

Fungal Enzymes as Catalytic Tools for Polyethylene Terephthalate (PET) Degradation

Ahmaditabatabaei

Kyazze

Iqbal

et al. 2021

JoF

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“…Since then, various effective enzymes have been observed to perform enzymatic PET hydrolysis. These enzymes with PET-degradation activity mainly belong to the subfamily of carboxylic ester hydrolases (EC 3.1.1.x), including carboxylesterase (EC 3.1.1.1) ( von Haugwitz et al, 2022 ), lipases ( Moyses et al, 2021 ), cutinases (EC 3.1.1.74) ( Wei and Zimmermann, 2017 ), and arylesterase (EC 3.1.1.2) ( Austin et al, 2018 ). In addition, several PETase-like enzymes have been identified, extending the scope of enzymatic PET recycling ( Almeida et al, 2019 ; Karunatillaka et al, 2022 ; Li et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Recent advances in the biodegradation of polyethylene terephthalate with cutinase-like enzymes

Sui,

Wang,

Fang

et al. 2023

Front. Microbiol.

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Polyethylene terephthalate (PET) is a synthetic polymer in the polyester family. It is widely found in objects used daily, including packaging materials (such as bottles and containers), textiles (such as fibers), and even in the automotive and electronics industries. PET is known for its excellent mechanical properties, chemical resistance, and transparency. However, these features (e.g., high hydrophobicity and high molecular weight) also make PET highly resistant to degradation by wild-type microorganisms or physicochemical methods in nature, contributing to the accumulation of plastic waste in the environment. Therefore, accelerated PET recycling is becoming increasingly urgent to address the global environmental problem caused by plastic wastes and prevent plastic pollution. In addition to traditional physical cycling (e.g., pyrolysis, gasification) and chemical cycling (e.g., chemical depolymerization), biodegradation can be used, which involves breaking down organic materials into simpler compounds by microorganisms or PET-degrading enzymes. Lipases and cutinases are the two classes of enzymes that have been studied extensively for this purpose. Biodegradation of PET is an attractive approach for managing PET waste, as it can help reduce environmental pollution and promote a circular economy. During the past few years, great advances have been accomplished in PET biodegradation. In this review, current knowledge on cutinase-like PET hydrolases (such as TfCut2, Cut190, HiC, and LCC) was described in detail, including the structures, ligand–protein interactions, and rational protein engineering for improved PET-degrading performance. In particular, applications of the engineered catalysts were highlighted, such as improving the PET hydrolytic activity by constructing fusion proteins. The review is expected to provide novel insights for the biodegradation of complex polymers.

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“…They are thus prone to attack high molecular weight (Mw) compounds and transport the small fragments through plasma membrane for further intracellular break down. Notably fungi are often regarded as better plastic degrader than bacteria as illustrated by Moyses et al 2021 ; Urbanek et al 2021 ; Radwan et al 2020 ; Muhonja et al 2018 . Scientists across the globe are hence on the lookout for potent fungi and utilize their prowess.…”

Section: Introductionmentioning

confidence: 99%

Myco-remediation of plastic pollution: current knowledge and future prospects

Khatua,

Simal-Gandara,

Acharya

2023

Biodegradation

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To date, enumerable fungi have been reported to participate in the biodegradation of several notorious plastic materials following their isolation from soil of plastic-dumping sites, marine water, waste of mulch films, landfills, plant parts and gut of wax moth. The general mechanism begins with formation of hydrophobin and biofilm proceding to secretion of specific plastic degarding enzymes (peroxidase, hydrolase, protease and urease), penetration of three dimensional substrates and mineralization of plastic polymers into harmless products. As a result, several synthetic polymers including polyethylene, polystyrene, polypropylene, polyvinyl chloride, polyurethane and/or bio-degradable plastics have been validated to deteriorate within months through the action of a wide variety of fungal strains predominantly Ascomycota (Alternaria, Aspergillus, Cladosporium, Fusarium, Penicillium spp.). Understanding the potential and mode of operation of these organisms is thus of prime importance inspiring us to furnish an up to date view on all the presently known fungal strains claimed to mitigate the plastic waste problem. Future research henceforth needs to be directed towards metagenomic approach to distinguish polymer degrading microbial diversity followed by bio-augmentation to build fascinating future of waste disposal.

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Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using Penicillium species

Cited by 25 publications

References 62 publications

Fungal Enzymes as Catalytic Tools for Polyethylene Terephthalate (PET) Degradation

Fungal Enzymes as Catalytic Tools for Polyethylene Terephthalate (PET) Degradation

Recent advances in the biodegradation of polyethylene terephthalate with cutinase-like enzymes

Myco-remediation of plastic pollution: current knowledge and future prospects

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