Fungal Biodiversity Profiles 31–40

Lactifluus indovolemus sp. nov. and two new records in the Lactifluus volemus complex (Basidiomycota, Russulaceae) from India

“…Genomic DNA was isolated from dry herbarium specimens (10-50 mg) using the Fungal gDNA Mini Kit (RGCB, RFDF, Thiruvananthapuram). PCR protocol for the amplification of ITS1, 5.8S and ITS2 regions (nrITS) followed those in Das et al (2017). PCR products were duly purified using a QIAquick Gel Extraction Kit (QIAGEN, Germany) and subjected to automated DNA sequencing on an ABI3730xl DNA Analyzer adding the same primers used earlier for amplification.…”

“…Lactifluus sect. Lactifluus (Van de Putte et al 2012, Das et al 2017, De Crop et al 2017), (b) BLAST (Altschul et al 1997) searches in the public database GenBank (Clark et al 2016) and (c) four sequences derived from our four collections. Multiple sequence alignment was performed using MAFFT ver.…”

“…Lactifluus sect. Lactifluus which is characterized by combination of the following characters: presence of convex pileus when young which gradually becomes applanate with a central depression to infundibuliform at maturity, velvety or dry surface with fine wrinkles at the margin, color of the pileus ranging from bright orange to dark orange-brown (sometimes striking pale yellow or brick-reddish), distinctive fishy odor, presence of abundant, sticky, white but turning into brown staining latex, blue-green reaction on application of FeSO 4 , presence of abundant lamprocystidia, lampropalisade structure of pilei-and stipitipellis and reticulate spore ornamentation (Verbeken 1998 (Berkeley 1852, Kuntze 1891, Taylor et al 2000, Van de Putte et al 2012, 2013, Verbeken et al 2012, Das et al 2017.…”

“…(m) Basidiospores. Scale bars: e-l = 10 µm, m = 2 µm.velutinose radially wrinkled pilear surface and larger basidiospores [8.3-9.7-10.4-12.8(-12.9) × 7.8-9.2-9.9-11.9 μm](Das et al 2017). Another Asian species, L. versiformis Van de Putte, K. Das & Verbeken, differs from L. indovolemus by taller stipe (55-115 × 15-25 mm) and longer pilear hairs (19-130 × 3-8 μm) (Van de Putte et al 2012).…”

Russula viridicinnamomea F. Yuan & Y. Song, sp. nov. and R. pseudocatillus F. Yuan & Y. Song, sp. nov., Two New Species from Southern China

“…A thorough survey for Russulaceae was carried out by the authors in DHSBR since 2014. As a result, eight new species and one epitype of Russula (Das et al 2017;Zhang et al 2017;Song et al 2018a,b), two new species of Lactarius (Wang et al 2018) and three new species and one new variety of Lactifluus (Song et al 2017(Song et al , 2018cZhang et al 2016) have been published. In this study, two additional novel Russula species, R. viridicinnamomea F. Yuan & Y.…”