Fungal biofactories as potential inulinase sources for production of fructooligosaccharides

Paul, Indrani; Kumar, C. Ganesh

doi:10.1016/b978-0-12-821007-9.00015-2

Cited by 6 publications

(3 citation statements)

References 284 publications

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“…The results of this study are different from the previous studies which found sources of inulinase from fungi such as the discovery of Aspergillus fumigatus [26], and Penicillium sp., as well as other sources of inulinase from bacteria such as Pseudomonas sp., Bacillus sp., and Arthrobacter sp, and source inulinase from yeasts such as Saccharomyces sp., Kluyveromyces sp., and Candida sp. [27].…”

Section: Discussionmentioning

confidence: 99%

“…Fungal growth as an inulinase producer needs attention, especially in temperature and pH regulation. Temperature and pH play an important role in the best inulinase production, which is in the range of PH 4-6.5 [9]. The results of the previous studies found that plant materials containing inulin such as dahlia, garlic, and Jerusalem artichoke could be used as a carbon source to produce the best inulinase compared to the purified substrate.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Molecular Identification of Endophytic Mold Schizophillum commune in Red Dahlia (Dahlia sp. L) Tuber as Producing Inulinases

Sunarti

Ginting

2022

Open Access Maced J Med Sci

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BACKGROUND: Dahlia tuber as the source of isolates in endophytic mold produces Inulinases. Inulinase is used in industry to produce inulin to become fructose through an enzymatic reaction. Fructose from natural substances constitutes low calories used as diet in diabetes mellitus type 2 patients. An important phase is done obtain the inulinase enzyme through mold isolation. AIM: The objective of this research was to isolate and identify the molecular of endophytic mold from red dahlia tuber which had the optimum inulinase activity. METHODS: Mold isolation was done through the stage of surface sterilization and purification. Mold isolation which had the highest inulinase activity was identified in a molecular using the polymerase chain reaction technique with the Internal transcribed spacers ITS region. DNA amplification used primer ITS 1 and ITS 4. RESULTS: The result of the study showed that there was five-mold isolation with the result of screening high inulinase activity found in UD3 (++), UD4 (++), and UD5 (+++), the value of inulinase enzyme activity of UD3 was 0.582716049, UD4 was 0.330864198, and UD5 was 0.685185185. The result of identifying UD5 molecular in eight DNA tapes was successfully amplified with 660 pb. The result of identifying molecular based on BLAST analysis found Schizophillum commune species CONCLUSION: It was found that the highest inulinase activity was the species S. commune, which added new variants of inulinase enzyme from red dahlia tuber which could be used by industry to yield fructose through an enzymatic reaction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Identification of Endophytic Mold Schizophillum commune in Red Dahlia (Dahlia sp. L) Tuber as Producing Inulinases

Sunarti

Ginting

2022

Open Access Maced J Med Sci

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“…The enzymes of the former class, hydrolyzing the non-reducing terminal residue of inulin, release fructose and are therefore used in the industry to obtain high-fructose syrup (HFS). On the other hand, endoinulinases break off inulin arbitrarily releasing FOS of different chain length [ 3 , 4 ]. Since FOS have great industrial importance owing to their functional properties and health benefits, recent developments in industrial biotechnology have enabled their large-scale production [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Production, Biochemical Characterization, and Kinetic/Thermodynamic Study of Inulinase from Aspergillus terreus URM4658

Oliveira¹,

Silva²,

Converti

et al. 2022

Molecules

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Inulinases are enzymes involved in the hydrolysis of inulin, which can be used in the food industry to produce high-fructose syrups and fructo-oligosaccharides. For this purpose, different Aspergillus strains and substrates were tested for inulinase production by solid-state fermentation, among which Aspergillus terreus URM4658 grown on wheat bran showed the highest activity (15.08 U mL−1). The inulinase produced by this strain exhibited optimum activity at 60 °C and pH 4.0. A detailed kinetic/thermodynamic study was performed on the inulin hydrolysis reaction and enzyme thermal inactivation. Inulinase was shown to have a high affinity for substrate evidenced by very-low Michaelis constant values (0.78–2.02 mM), which together with a low activation energy (19.59 kJ mol−1), indicates good enzyme catalytic potential. Moreover, its long half-life (t1/2 = 519.86 min) and very high D-value (1726.94 min) at 60 °C suggested great thermostability, which was confirmed by the thermodynamic parameters of its thermal denaturation, namely the activation energy of thermal denaturation (E*d = 182.18 kJ mol−1) and Gibbs free energy (106.18 ≤ ΔG*d ≤ 111.56 kJ mol−1). These results indicate that A. terreus URM4658 inulinase is a promising and efficient biocatalyst, which could be fruitfully exploited in long-term industrial applications.

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Production of Inulin Oligosaccharides from Microbial Inulinases and Their Applications

Priyanka,

Ghai,

Azmi

2024

Microbial Enzymes

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Fungal biofactories as potential inulinase sources for production of fructooligosaccharides

Cited by 6 publications

References 284 publications

Isolation and Molecular Identification of Endophytic Mold Schizophillum commune in Red Dahlia (Dahlia sp. L) Tuber as Producing Inulinases

Isolation and Molecular Identification of Endophytic Mold Schizophillum commune in Red Dahlia (Dahlia sp. L) Tuber as Producing Inulinases

Production, Biochemical Characterization, and Kinetic/Thermodynamic Study of Inulinase from Aspergillus terreus URM4658

Production of Inulin Oligosaccharides from Microbial Inulinases and Their Applications

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