Fungal biofilms in the hemodialysis environment

Oliveira, Lariane Teodoro; Lopes, Leonardo Guedes; Ramos, Salvador Boccaletti; Martins, Carlos Henrique Gomes; Jamur, Maria Célia; Pires, Regina Helena

doi:10.1016/j.micpath.2018.07.018

Cited by 13 publications

(9 citation statements)

References 55 publications

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“…The results obtained in the quantification of the biomass by the crystal violet test, showed a stationary phase in the development of the biomass after 144 h and greater amount of mass in biofilms formed in HAM-F12 medium in the condition of microaerophilia, showing a concordance in what was observed in the XTT assay. Crystal violet is a dye widely used in fungal and bacterial biofilms ( Costa-Orlandi et al, 2014 ; Oliveira et al, 2018 ; Xu et al, 2019 ). Basically, it is an assay able to quantify biomass.…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

et al. 2020

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Histoplasma capsulatum is a dimorphic fungus that causes an important systemic mycosis called histoplasmosis. It is an infectious disease with high prevalence and morbidity that affects the general population. Recently, the ability of these fungi to form biofilms, a phenotype that can induce resistance and enhance virulence, has been described. Despite some efforts, data regarding the impact of nutrients and culture media that affect the H. capsulatum biofilm development in vitro are not yet available. This work aimed to study H. capsulatum biofilms, by checking the influence of different culture media and oxygen atmospheres in the development of these communities. The biofilm formation by two strains (EH-315 and G186A) was characterized under different culture media: [Brain and Heart Infusion (BHI), Roswell Park Memorial Institute (RPMI) with 2% glucose, Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum and nutrient medium HAM-F12 (HAM-F12) supplemented with glucose (18.2 g/L), glutamic acid (1 g/L), HEPES (6 g/L) and L-cysteine (8.4 mg/L)] and oxygen atmospheres (aerobiosis and microaerophilia), using the XTT reduction assay to quantify metabolic activities, crystal violet staining for biomass, safranin staining for the quantification of polysaccharide material and scanning electron microscopy (SEM) for the observation of topographies. Results indicated that although all culture mediums have stimulated the maturation of the communities, HAM-F12 provided the best development of biomass and polysaccharide material when compared to others. Regarding the oxygen atmospheres, both stimulated an excellent development of the communities, however in low oxygen conditions an exuberant amount of extracellular matrix was observed when compared to biofilms formed in aerobiosis, mainly in the HAM-F12 media. SEM images showed yeasts embedded by an extracellular matrix in several points, corroborating the colorimetric assays. However, biofilms formed in BHI, RPMI, and DMEM significantly induced yeast to hyphae reversal, requiring further investigation. The results obtained so far contribute to in vitro study of biofilms formed by these fungi and show that nutrition promoted by different media modifies the development of these communities. These data represent advances in the field of biofilms and contribute to future studies that can prove the role of these communities in the fungi-host interaction.

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Section: Discussionmentioning

confidence: 99%

Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

et al. 2020

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“… 55 Critically ill patients with renal failure are at risk for ICI because of immune dysfunction 54 and renal replacement therapy via a vascular catheter instead of a shunt. 64 Other factors (eg, contamination of the dialysate, colonization of the dialysis machines) have been described for the intermittent dialysis setting 65 , 66 but have not been assessed for the continuous or slow extended daily dialysis on the ICU. Patients with sepsis or septic shock exhibit several risks for developing ICU including antibiotic therapy, invasive therapeutic strategies (implanted medical devices, surgery for source control), gut barrier dysfunction, 67 and sepsis-induced immunosuppression.…”

Section: Discussionmentioning

confidence: 99%

Risk Factors for Invasive Candida Infection in Critically Ill Patients

Thomas-Rüddel

Schlattmann

Pletz

et al. 2022

CHEST

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“…Pulmonary aspergillosis is caused by the conidial saprophytic fungus Aspergillus , found in the soil and the air, dust from civil construction, and medical dispositive, which affects immunocompromised patients or those with pre-existing lung disease [ 20 , 50 ]. The main pathologies related to pulmonary aspergillosis are allergic bronchopulmonary aspergillosis (ABPA), chronic pulmonary aspergillosis (CPA), and invasive pulmonary aspergillosis (IPA), the determinant of these infections being the interactive relationship between the fungus and the host [ 51 ].…”

Section: Fungal Infectionsmentioning

confidence: 99%

Nanotechnology-Based Approaches for Voriconazole Delivery Applied to Invasive Fungal Infections

et al. 2023

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Invasive fungal infections increase mortality and morbidity rates worldwide. The treatment of these infections is still limited due to the low bioavailability and toxicity, requiring therapeutic monitoring, especially in the most severe cases. Voriconazole is an azole widely used to treat invasive aspergillosis, other hyaline molds, many dematiaceous molds, Candida spp., including those resistant to fluconazole, and for infections caused by endemic mycoses, in addition to those that occur in the central nervous system. However, despite its broad activity, using voriconazole has limitations related to its non-linear pharmacokinetics, leading to supratherapeutic doses and increased toxicity according to individual polymorphisms during its metabolism. In this sense, nanotechnology-based drug delivery systems have successfully improved the physicochemical and biological aspects of different classes of drugs, including antifungals. In this review, we highlighted recent work that has applied nanotechnology to deliver voriconazole. These systems allowed increased permeation and deposition of voriconazole in target tissues from a controlled and sustained release in different routes of administration such as ocular, pulmonary, oral, topical, and parenteral. Thus, nanotechnology application aiming to delivery voriconazole becomes a more effective and safer therapeutic alternative in the treatment of fungal infections.

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Fungal biofilms in the hemodialysis environment

Cited by 13 publications

References 55 publications

Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

Risk Factors for Invasive Candida Infection in Critically Ill Patients

Nanotechnology-Based Approaches for Voriconazole Delivery Applied to Invasive Fungal Infections

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