Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Lübeck, Mette; Lübeck, Peter Stephensen

doi:10.3390/microorganisms10040753

Cited by 76 publications

(42 citation statements)

References 176 publications

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“…Filamentous fungi rely on external carbon sources because they are heterotrophic, but many can absorb inorganic nitrogen and other nutrients for growth and metabolism. They can make all the necessary amino acids from inorganic nitrogen [29]. Many protoplast isolation studies indicated that 0.6 M KCl acts as the best osmotic stabilizer [15,18].…”

Section: Discussionmentioning

confidence: 99%

Enhancement of pigment and proximate values of intergeneric hybrid of protoplast fusion of Monascus ruber and Pleurotus ostreatus

Kannan

Nanthini

2022

Preprint

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Protoplast fusion is a crucial technique for strain enhancement that brings genetic recombination to filamentous fungus and creates hybrid strains. In this present study, the intergenic fusion between M. ruber and P. ostreatus has been carried out to enhance pigments, secondary metabolites, and proximate values. Protoplasts from M. ruber and P. ostreatus were isolated and an equal amount of protoplast was taken and performed protoplast fusion. The regenerated colonies were plated in potato dextrose agar with selected biological markers (nystatin and fluconazole) for the confirmation of the hybrid. In a standardized condition, the protoplast yield of M. ruber and P. ostreatus was 2.31×107 cells/ml and 2.33×107 cells/ml, respectively. Acidic pH yielded high amounts of protoplasts than neutral and alkaline pH. KCl showed a high yield of protoplasts 2.36×106 cells /ml from M. ruber and 2.46×107cells/ml from P. ostreatus. The colony resistance to nystatin and fluconazole was confirmed that the obtained regenerated colonies were fusant. The fusant has no septation in its transverse walls with lipid droplets. Fusion of protoplast from M. ruber and P. ostreatus resulted in genetic inference at the metabolic levels, explaining the elevation of pigment in fusant colonies. The proximate composition of fusant strains was higher. In conclusion, we have developed a new strain with genetic variance capable of producing an increased level of pigment with nutritional value through protoplast fusion that allows the generation of fusant with the characteristics of parent strains and elucidates the effectiveness of fusant for commercial applications.

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Section: Discussionmentioning

confidence: 99%

Enhancement of pigment and proximate values of intergeneric hybrid of protoplast fusion of Monascus ruber and Pleurotus ostreatus

Kannan

Nanthini

2022

Preprint

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“…Mycoprotein Quorn contains protein (45%), fiber (25%), fat (13%) and carbohydrates (10%) in 100 grams of dry weight [15]. Numerous fungi that could produce mycoproteins include Rhizopus oligosporus, Aspergillus niger, and Neurospora sitophila [16]. Yeasts Saccharomyces cerevisiae and Kluyveromyces marxianus are capable of producing SCP [17].…”

Section: Corynobacterium Ammoniagenesmentioning

confidence: 99%

“…Fungi need organic matter as the primary source of carbon for energy production. There are at least eight macronutrients that need to be met: carbon, oxygen, hydrogen, nitrogen, phosphorus, potassium, sulfur, and magnesium, and there are five micronutrients: manganese, iron, zinc, copper, and molybdenum [16]. Organic carbon sources are sugars, such as glucose, fructose, and sucrose.…”

Section: Fermentation Processmentioning

confidence: 99%

Quality Aspects Related to Meat Analogue Based on Microbiology, Plants and Insects Protein

Yusuf¹,

Setiarto²

2022

RAS

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Rapid global population growth has caused an increasing global consumption of meat and has resulted in the surging demand for meat analogue products over the last few years. There are many reasons why consumers and food producers are looking for alternatives to meat and meat products, including environmental, health, and ethical aspects. This study reviews recent scientific reports on quality aspects related to meat analogue based on single cell protein, insect protein and plant protein. The scope of the review includes the following: alternative protein sources, composition and nutritional value, and processing technologies of meat analogue. Protein from single cell protein (algae, mold, yeast, bacteria), plants (soybean, wheat gluten, legumes), and insects were described. The need for further research in this area, particularly on the nutritional value, technology for producing meat analogue, and food safety of meat analogue, was demonstrated.

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“…Extracellularly secreted enzymes are more convenient for subsequent enzyme separation and extraction than intracellularly produced enzymes. In addition, the cellulase produced by fungi has a more reasonable structure, and the decomposition efficiency of the enzyme is higher [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Plasma Promotes Fungal Cellulase Production by Regulating the Levels of Intracellular NO and Ca2+

Ketya

Choi

et al. 2022

IJMS

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For the industrial-scale production of useful enzymes by microorganisms, technological development is required for overcoming a technical bottleneck represented by poor efficiency in the induction of enzyme gene expression and secretion. In this study, we evaluated the potential of a non-thermal atmospheric pressure plasma jet to improve the production efficiency of cellulolytic enzymes in Neurospora crassa, a filamentous fungus. The total activity of cellulolytic enzymes and protein concentration were significantly increased (1.1~1.2 times) in media containing Avicel 24–72 h after 2 and 5 min of plasma treatment. The mRNA levels of four cellulolytic enzymes in fungal hyphae grown in media with Avicel were significantly increased (1.3~17 times) 2–4 h after a 5 min of plasma treatment. The levels of intracellular NO and Ca2+ were increased in plasma-treated fungal hyphae grown in Avicel media after 48 h, and the removal of intracellular NO decreased the activity of cellulolytic enzymes in media and the level of vesicles in fungal hyphae. Our data suggest that plasma treatment can promote the transcription and secretion of cellulolytic enzymes into the culture media in the presence of Avicel (induction condition) by enhancing the intracellular level of NO and Ca2+.

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Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

Cited by 76 publications

References 176 publications

Enhancement of pigment and proximate values of intergeneric hybrid of protoplast fusion of Monascus ruber and Pleurotus ostreatus

Enhancement of pigment and proximate values of intergeneric hybrid of protoplast fusion of Monascus ruber and Pleurotus ostreatus

Quality Aspects Related to Meat Analogue Based on Microbiology, Plants and Insects Protein

Plasma Promotes Fungal Cellulase Production by Regulating the Levels of Intracellular NO and Ca2+

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