Fungal cellulases: protein engineering and post-translational modifications

Zhang, Ruiqin; Cao, Cheng-Hao; Bi, Jiahua; Li, Yanjun

doi:10.1007/s00253-021-11723-y

Cited by 20 publications

(9 citation statements)

References 226 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the period before 2015, such studies are extensively reviewed in Payne et al (2015) . Recent reviews with more or less comprehensive overviews of more recent cellulase engineering studies date from 2020 ( Contreras et al , 2020a ) and 2021 ( Zhang et al , 2021 ). These include tables that list, and shortly describe, individual studies published, primarily, in the period of 2010–2020.…”

Section: Protein Engineering Of Cellulolytic Enzymesmentioning

confidence: 99%

“…Fungal cellulases and their engineering have been reviewed by Payne et al (2015) in an impressively comprehensive review that addresses many (general) aspects of cellulase structure and function in much detail. Other useful reviews focusing on engineering cellulases include ( Bommarius et al , 2014 , Greene et al , 2015 , Contreras et al , 2020a , Zhang et al , 2021 ). In this short review, we discuss engineering strategies used for increasing the industrial performance of cellulases, presenting a few selected highlights from the past and recent advancements.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Engineering cellulases for conversion of lignocellulosic biomass

Chaudhari

Várnai

Sørlie

et al. 2023

Protein Engineering, Design and Selection

View full text Add to dashboard Cite

Lignocellulosic biomass is a renewable source of energy, chemicals, and materials. Many applications of this resource require the depolymerization of one or more of its polymeric constituents. Efficient enzymatic depolymerization of cellulose to glucose by cellulases and accessory enzymes such as lytic polysaccharide monooxygenases (LPMOs) is a prerequisite for economically viable exploitation of this biomass. Microbes produce a remarkably diverse range of cellulases, which consist of glycoside hydrolase (GH) catalytic domains and, although not in all cases, substrate-binding carbohydrate-binding modules (CBMs). Since enzymes are a considerable cost factor, there is great interest in finding or engineering improved and robust cellulases, with higher activity and stability, easy expression, and minimal product inhibition. This review addresses relevant engineering targets for cellulases, discusses a few notable cellulase engineering studies of the past decades, and provides an overview of recent work in the field.

show abstract

Section: Protein Engineering Of Cellulolytic Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Engineering cellulases for conversion of lignocellulosic biomass

Chaudhari

Várnai

Sørlie

et al. 2023

Protein Engineering, Design and Selection

View full text Add to dashboard Cite

show abstract

“…Hence, engineering individual lignocellulose deconstructing enzymes has been a topic of intense research. For instance, cellulases have been optimised for improved activity, thermostability and pH tolerance; the details of these efforts are outside the scope of this piece, but many of these efforts are covered in excellent reviews [86–89] . Engineered enzyme cocktails have also been developed, with multiple synergistic biocatalysts working together to deconstruct several biomass components in parallel [90] .…”

Section: Accessing Renewable Feedstocks and Chemicals From Biological...mentioning

confidence: 99%

“…For instance, cellulases have been optimised for improved activity, thermostability and pH tolerance; the details of these efforts are outside the scope of this piece, but many of these efforts are covered in excellent reviews. [ 86 , 87 , 88 , 89 ] Engineered enzyme cocktails have also been developed, with multiple synergistic biocatalysts working together to deconstruct several biomass components in parallel. [90] These cocktails typically consist of cellulases, cellobiohydrolases, endoglucanases, β‐glucosidases, hemicellulases, and lytic polysaccharide monooxygenases.…”

Section: Accessing Renewable Feedstocks and Chemicals From Biological...mentioning

confidence: 99%

Engineering Enzymes for Environmental Sustainability

Radley,

Davidson,

Foster

et al. 2023

Angew Chem Int Ed

View full text Add to dashboard Cite

The development and implementation of more efficient and sustainable technologies is key to delivering our net‐zero targets. Here we review how engineered enzymes, with a focus on those developed using directed evolution, can be deployed to improve the sustainability of numerous processes and help to conserve our environment. Efficient and robust biocatalysts have been engineered to capture carbon dioxide (CO2) and have been embedded into new efficient metabolic CO2 fixation pathways. Enzymes have been refined for bioremediation, enhancing their ability to degrade toxic and harmful pollutants. Biocatalytic recycling is gaining momentum, with engineered cutinases and PETases developed for the depolymerization of the abundant plastic, PET. Finally, biocatalytic approaches for accessing petroleum‐based feedstocks and chemicals are expanding, using optimized enzymes to convert plant biomass into biofuels or other high value products. Through these examples, we hope to illustrate how enzyme engineering and biocatalysis can contribute to the development of more environmentally sustainable approaches, in order to protect our planet.

show abstract

“…Cellulase acts on the β-1,4-glucosidic bond and is a multicomponent enzyme with multiple action sites ( Zhang, Cao, Bi, & Li, 2022 ). The enzymatic method has several advantages over chemical and physical methods, including low addition, low energy consumption, low pollution levels, and high safety ( de Souza & Kawaguti, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of cellulase on antioxidant activity and flavor of Rosa roxburghii Tratt

Jiang,

Li,

Ding

et al. 2024

Food Chemistry: X

View full text Add to dashboard Cite

Fungal cellulases: protein engineering and post-translational modifications

Cited by 20 publications

References 226 publications

Engineering cellulases for conversion of lignocellulosic biomass

Engineering cellulases for conversion of lignocellulosic biomass

Engineering Enzymes for Environmental Sustainability

Effect of cellulase on antioxidant activity and flavor of Rosa roxburghii Tratt

Contact Info

Product

Resources

About