Fungal Community Composition at the Last Remaining Wild Site of Yellow Early Marsh Orchid (Dactylorhiza incarnata ssp. ochroleuca)

Dove, Andrea; Charters, Michael D.; Campbell, Matthew J.; Blake, Hanna; Menon, Manoj; Sarasan, Viswambharan

doi:10.3390/microorganisms11082124

Cited by 3 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In some cases, selected groups of fungi are in decline in some habitats, as reported before [18]. Several studies undertaken at a fine-scale habitat and at a large landscape level for monitoring of species have found that relevant environmental data for vegetation niche modelling and community composition of fungi [13,15,[19][20][21][22][23] are examples that can help develop strategies for Conservation Translocation of rare orchids. Species like GWO prefer unimproved grasslands that are either managed by grazing or mowing, and orchids prefer non-dominant vegetation.…”

Section: Introductionmentioning

confidence: 83%

“…Ascomycetous fungal groups such as Helotiales and Hypocreales are known to be distributed abundantly in orchid habitats [15]. On the other hand, Basidiomycetous fungi with most of the orchid mycorrhizal fungal groups can be considered 'rare' fungi [33].…”

Section: Discussionmentioning

confidence: 99%

“…DNA metabarcoding was conducted by Nature Metrics (Guildford, UK), as described by Dove et al [15]. Detailed methodology is as follows.…”

Section: Metabarcodingmentioning

confidence: 99%

“…But at the local fine-scale levels, spatial distribution patterns may be driven by other factors, which leads to small population sizes and a scattered occurrence. In addition to the support from OMFs, an understanding of abiotic factors is also important to orchid recovery and establishment in the wild [15][16][17]. In some cases, selected groups of fungi are in decline in some habitats, as reported before [18].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Soil Studies for Fungal Diversity to Enable the Conservation Translocation of Green-Winged Orchid

Newmarch,

Velde,

Menon

et al. 2024

Diversity

Self Cite

View full text Add to dashboard Cite

Conservation Translocation (CT), which includes reintroduction, reinforcement and introduction, is gaining momentum, responding to serious population decline in many orchids. Orchid conservation underpinned by a greater emphasis on understanding biotic and abiotic factors of habitats is critical for successful recovery and restoration programmes. Anacamptis morio, commonly known as green-winged orchid (GWO), is a terrestrial orchid found throughout Europe, but populations in England rapidly declined in its native range in the last several decades. The current study explored the relationship between soil abiotic characteristics and the community composition of key fungal groups. Wild sites in Essex and Cumbria in England, where successful colonies are currently present, were compared to potential sites for CT in Cumbria. The Cumbrian sites managed by cattle grazing include the wild site and three potential CT sites, with two of them hosting no GWO plants. The Essex site, fOxley Meadow, where no cattle is used for grassland management, hosts the largest population of GWO in England. The aim of this study was to understand whether the community composition of fungi and soil characteristics of the potential CT sites in Cumbria are nearly compatible with that of Oxley Meadow. Oxley Meadow, with around 65,000 plants, stands out as a unique habitat compared to all Cumbria sites, as it showed low organic content. Nitrate and phosphate content were smaller for Oxley Meadow compared to other Cumbrian sites. The proportion of Basidiomycota fungi was greater in Oxley Meadow compared to all Cumbrian sites where Ascomycota dominated. The abundant fungal group found in Oxley Meadow was Agaricales. From Agariclaes, Hygrophoraceae or waxcaps fungi are considered an indicator group of fungi and were the most abundant group in Oxley Meadow. They have a negative correlation with targeted key fungal groups and abiotic parameters. More in-depth assessments using additional primers are essential to better understand the fungal diversity and how this diversity translates to the resilience of orchid habitats. This preliminary study points to future studies to assess whether sites are near-compatible to the wild site where large colonies are present using additional primers collected over different time scales.

show abstract

Section: Introductionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

“…DNA metabarcoding was conducted by Nature Metrics (Guildford, UK), as described by Dove et al [15]. Detailed methodology is as follows.…”

Section: Metabarcodingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Soil Studies for Fungal Diversity to Enable the Conservation Translocation of Green-Winged Orchid

Newmarch,

Velde,

Menon

et al. 2024

Diversity

Self Cite

View full text Add to dashboard Cite

show abstract

Fungal community characteristics of the last remaining habitat of three paphiopedilum species in China

Tian,

An,

Liu

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Paphiopedilum armeniacum, Paphiopedilum wenshanense and Paphiopedilum emersonii are critically endangered wild orchids. Their populations are under severe threat, with a dramatic decline in the number of their natural distribution sites. Ex situ conservation and artificial breeding are the keys to maintaining the population to ensure the success of ex situ conservation and field return in the future. The habitat characteristics and soil nutrient information of the last remaining wild distribution sites of the three species were studied. ITS high-throughput sequencing was used to reveal the composition and structure of the soil fungal community, analyze its diversity and functional characteristics, and reveal its relationship with soil nutrients. The three species preferred to grow on low-lying, ventilated and shaded declivities with good water drainage. There were significant differences in soil alkali-hydrolyzed nitrogen and available phosphorus among the three species. There were 336 fungal species detected in the samples. On average, there were different dominant groups in the soil fungal communities of the three species. The functional groups of soil fungi within their habitats were dominated by saprophytic fungi and ectomycorrhizae, with significant differences in diversity and structure. The co-occurrence network of habitat soil fungi was mainly positive. Soil pH significantly affected soil fungal diversity within their habitats of the three paphiopedilum species. The study confirmed that the dominant groups of soil fungi were significantly correlated with soil nutrients. The three species exhibit comparable habitat inclinations, yet they display substantial variations in the composition, structure, and diversity of soil fungi. The fungal functional group is characterized by a rich presence of saprophytic fungi, a proliferation of ectomycorrhizae, and a modest occurrence of orchid mycorrhizae. The symbiotic interactions among the soil fungi associated with these three species are well-coordinated, enhancing their resilience against challenging environmental conditions. There is a significant correlation between soil environmental factors and the composition of soil fungal communities, with pH emerging as a pivotal factor regulating fungal diversity. Our research into the habitat traits and soil fungal ecosystems of the three wild Paphiopedilum species has established a cornerstone for prospective ex situ conservation measures and the eventual reestablishment of these species in their native landscapes. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-75185-8.

show abstract

Analysis of the Fungal Community Composition in Endemic Orchids with Terrestrial Habitat in Subtropical Regions

Hu,

Qi,

Artigas Ramírez

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Habenaria and Liparis are well-known orchid genera that grow in terrestrial habitats in the tropics, subtropics or temperate zones. Three species have been found in subtropical regions of China, inhabiting terrestrial to epiphytic habitats. This study focuses on three species, H. dentata (distributed in Asia), H. yachangensis, and L. gigantea. For H. yachangensis and L. gigantea, there is no information about the mycorrhizal community in these species. This study aims to conduct the fungal community screening of Chinese ground orchids from subtropical regions. We performed a comparative analysis of the fungal community among H. dentata, H. yachangensis, and L. gigantea, determining their ITS regions using NGS paired-end sequences. The results clarified the diversity and the predominance of fungal genera. Ascomycota was abundant compared to Basidiomycota or other fungi groups in all communities, with a high dominance in all populations, especially for L. gigantea. At different root spatial locations, the fungal community diversity and richness were higher in the soil than in the rhizosphere or inner root. However, the results suggest that L. gigantea has a different fungal community compared to Habenaria species. In this order, the subtropical terrestrial orchids have a different fungal network compared to the northern terrestrial orchids. Also, there is a high probability of co-existence and co-evolution of endophytic fungi in these terrestrial orchids, indicating the potential role of host plants in selecting an endophytic fungal community. Furthermore, our results highlight the need to elucidate the microbe interactions of these unique orchids for long-term purposes, such as isolating indigenous fungi for suitable inoculants for further orchid propagation, restoration, and conservation.

show abstract

Fungal Community Composition at the Last Remaining Wild Site of Yellow Early Marsh Orchid (Dactylorhiza incarnata ssp. ochroleuca)

Cited by 3 publications

References 42 publications

Soil Studies for Fungal Diversity to Enable the Conservation Translocation of Green-Winged Orchid

Soil Studies for Fungal Diversity to Enable the Conservation Translocation of Green-Winged Orchid

Fungal community characteristics of the last remaining habitat of three paphiopedilum species in China

Analysis of the Fungal Community Composition in Endemic Orchids with Terrestrial Habitat in Subtropical Regions

Contact Info

Product

Resources

About