Fungal community in olive fruits of cultivars with different susceptibilities to anthracnose and selection of isolates to be used as biocontrol agents

Preto, G. A. S.; Martins, Fátima; Pereira, José Alberto; Baptista, Paula

doi:10.1016/j.biocontrol.2017.03.011

Cited by 43 publications

(39 citation statements)

References 34 publications

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“…The endophytic fungal communities earlier found in aboveground olive tree compartments (phyllosphere and carposphere) by high-throughput sequencing 23 or a culture-dependent approach 20 , differed from belowground communities reported in our study. This outcome reinforces previous reports showing important differences between above- and belowground olive fungal communities, regardless the methodological approach implemented to study them 20,22,23 . Interestingly enough, Sordariomycetes was also the most abundant class found in olive fruits regardless or not the presence of anthracnose symptoms 23 , pointing to the fact that this fungal class seems to be ubiquitously colonizing the interior of olive tissues.…”

Section: Discussionsupporting

confidence: 90%

“…Cobrançosa 20 were screened by a culture-dependent method to compare the fungal communities between above- and belowground compartments. Microbial communities of the olive phyllosphere and carposphere have been analyzed using denaturing gradient gel electrophoresis (DGGE) 21 , isolation of fungi in culturing media 22 and high-throughput sequencing of both fungal 23 and prokaryotic 24 communities.…”

Section: Introductionmentioning

confidence: 99%

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Defining the root endosphere and rhizosphere microbiomes from the World Olive Germplasm Collection

Fernández-González¹,

Villadas²,

Cabanás

et al. 2019

Preprint

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Up to date, the bacterial and fungal microbial communities from the olive (Olea europaea L.) root systems have not been simultaneously studied. In this work, we show that microbial communities from the olive root endosphere are less diverse than those from the rhizosphere. But more relevant was to unveil that olive belowground communities are mainly shaped by the genotype of the cultivar when growing under the same environmental, pedological and agronomic conditions. Furthermore, Actinophytocola, Streptomyces and Pseudonocardia are the most abundant bacterial genera in the olive root endosphere, Actinophytocola being the most prevalent genus by far. In contrast, Gp6, Gp4, Rhizobium and Sphingomonas are the main genera in the olive rhizosphere. Canalisporium, Aspergillus, Minimelanolocus and Macrophomina are the main fungal genera present in the olive root system. Interestingly enough, a high proportion of so far unclassified fungal sequences at class level were detected in the rhizosphere. From the belowground microbial profiles here reported, it can be concluded that the genus Actinophytocola may play an important role in olive adaptation to environmental stresses. Moreover, the huge unknown fungal diversity suggests that there are still some fungi with important ecological and biotechnological implications that have yet to be discovered.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Defining the root endosphere and rhizosphere microbiomes from the World Olive Germplasm Collection

Fernández-González¹,

Villadas²,

Cabanás

et al. 2019

Preprint

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“…While studies on specific beneficial components of the olive-associated microbiota have been conducted, some of them aiming to isolate and characterize biological control agents (BCA) against VWO [12][13][14], only very few examples are available on whole indigenous olive microbial communities [15,16] and their potential relationship with susceptibility to biotic constraints [17]. Recently, we described the belowground microbial communities of a range of olive cultivars from different geographical origin grown under the same climatic, agronomical and soil conditions, and in the absence of V. dahliae pressure [18].…”

Section: Introductionmentioning

confidence: 99%

Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive

et al. 2020

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Background: Verticillium wilt of olive (VWO) is caused by the soilborne fungal pathogen Verticillium dahliae. One of the best VWO management measures is the use of tolerant/resistant olive cultivars. Knowledge on the oliveassociated microbiome and its potential relationship with tolerance to biotic constraints is almost null. The aims of this work are (1) to describe the structure, functionality, and co-occurrence interactions of the belowground (root endosphere and rhizosphere) microbial communities of two olive cultivars qualified as tolerant (Frantoio) and susceptible (Picual) to VWO, and (2) to assess whether these communities contribute to their differential disease susceptibility level. Results: Minor differences in alpha and beta diversities of root-associated microbiota were detected between olive cultivars regardless of whether they were inoculated or not with the defoliating pathotype of V. dahliae. Nevertheless, significant differences were found in taxonomic composition of non-inoculated plants' communities, "Frantoio" showing a higher abundance of beneficial genera in contrast to "Picual" that exhibited major abundance of potential deleterious genera. Upon inoculation with V. dahliae, significant changes at taxonomic level were found mostly in Picual plants. Relevant topological alterations were observed in microbial communities' co-occurrence interactions after inoculation, both at structural and functional level, and in the positive/negative edges ratio. In the root endosphere, Frantoio communities switched to highly connected and low modularized networks, while Picual communities showed a sharply different behavior. In the rhizosphere, V. dahliae only irrupted in the microbial networks of Picual plants. Conclusions: The belowground microbial communities of the two olive cultivars are very similar and pathogen introduction did not provoke significant alterations in their structure and functionality. However, notable differences were found in their networks in response to the inoculation. This phenomenon was more evident in the root endosphere communities. Thus, a correlation between modifications in the microbial networks of this microhabitat and susceptibility/tolerance to a soilborne pathogen was found. Moreover, V. dahliae irruption in the Picual microbial networks suggests a stronger impact on the belowground microbial communities of this cultivar upon inoculation. Our results suggest that changes in the co-occurrence interactions may explain, at least partially, the differential VWO susceptibility of the tested olive cultivars.

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“…A scanning electron microscope showed that the Trichoderma endophytes cause deformities in the mycelia of Pythium aphanidermatum and Rhizoctonia solani, such as hyphal fragmentation, perforation, lysis, and mycelial degeneration [28]. A strain of Trichoderma harzianum showed in vitro growth contact points that suggest mycoparasitic activity against Fusarium solani [34]. Endophytic and epiphytic fungi isolated from fruits of organic Olea europaea were able to inhibit mycelial growth, germination, and sporulation and cause pathogenic hyphae abnormalities of Colletotrichum acutatum, particularly at mycelial contact [35].…”

Section: Biological Controlmentioning

confidence: 99%

Endophytes Potential Use in Crop Production

Tonial¹,

Nava²,

Gayger³

et al. 2020

Sustainable Crop Production

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The endophytic microorganisms have the potential to improve the yield of agricultural crops. They can be used as biological control, plant growth promoter, or bioremediators. The action of endophytes in controlling phytopathogens, insects, and weeds that harm agriculture may be the result of microbial interactions with other organisms or the production of bioactive metabolites. Also, microorganisms can have the ability to favor plant growth and convert toxic compounds present in the soil. The presence of pollutants in the substrate reduces its quality for plant development, so bioremediation also impacts agricultural production. Therefore, prospecting endophytic microorganisms with agronomic potential may provide sustainable alternatives to increase crop yield.

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Fungal community in olive fruits of cultivars with different susceptibilities to anthracnose and selection of isolates to be used as biocontrol agents

Cited by 43 publications

References 34 publications

Defining the root endosphere and rhizosphere microbiomes from the World Olive Germplasm Collection

Defining the root endosphere and rhizosphere microbiomes from the World Olive Germplasm Collection

Linking belowground microbial network changes to different tolerance level towards Verticillium wilt of olive

Endophytes Potential Use in Crop Production

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