Fungal Diversity in the Soil of the Oxytropis glacialis Root System on the Qinghai-Tibet Plateau

Abstract

Because of swainonine-producing endophytic fungal, Oxytropis glacialis is one of the main poisonous weeds in the alpine grassland and desert grassland of the Qinghai-Tibet Plateau (QTP). It has a severe impact on grassland degradation on the QTP. In this manuscript, the Internally Transcribed Spacer (ITS) region of fungal communities in the soil of the O. glacialis root system was sequenced by high-throughput sequencing and analyzed by bioinformatics methods. The physical and chemical properties of the soil samples were analyzed in combination with the fungal diversity and its relationship with the soil physical and chemical factors. The results showed that the soil fungal community in the O. glacialis root system are rich in diversity in different ecological environments and are most affected by the soil pH value and organic matter. The swainonine-producing fungal Embellisia oxytropis was first detected in the soil of the O. glacialis root system. This finding provides data to support the next step in demonstrating the horizontal spread of swainone-producing fungal from O. glacialis to soil. In addition, a stable network of core flora has a facilitating effect on the formation of O. glacialis as a dominant species in alpine ecosystems.

High-throughput sequencing-based analysis of the composition and diversity of the endophyte community in roots of Stellera chamaejasme

“…In addition, among the three sample points, the NMC sample point has the highest pH and salinity value (pH 8.5, salinity 3 ms/cm), followed by XGYZ (pH 8.2, salinity 0.05 ms/cm). cm) and BGC (pH 7.4, salinity 0.11 ms/cm) 44 , 45 . Correspondingly, the Shannon diversity index NMC of the endophyte S. chamaejasme is the highest, followed by XGYZ and BGC; the ACE index NMC is the highest, followed by XGYZ and BGC; the richness index (especially bacteria) is also the highest in NMC, followed by XGYZ and BGC; this shows that pH and salinity are also closely related to the community structure and diversity of endophytic bacteria in S. chamaejasme .…”

Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau

“…Soil moisture was tested with the drying method: 5 g of soil was placed in a drying oven for 1 h and then weighed. The pH was tested using pH equipment (Sartorius PB-10), and the water-soil ratio was 1:1 ( Cao et al, 2022 ).…”

“…A total of 100 randomization interactions were performed and the multiple test correction based on Benjamini-Hochberg was used. When the net sum of the relationships is positive, the impact is synergistic, and when it is negative, the impact of the relationship is antagonistic ( Fath & Patten, 1998 ; Cao et al, 2022 ).…”