Fungal dye decolourization: Recent advances and future potential

Kaushik, Prachi; Malik, Anushree

doi:10.1016/j.envint.2008.05.010

Cited by 561 publications

(307 citation statements)

References 137 publications

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“…The maximum absorbance of RB5 was at 596 nm. It was characterized by the long conjugated π-system that linking the two azo bond of RB5 (Kaushik and Malik 2009). The calibration curve that is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Microbial Decolorization of an Azo Dye Reactive Black 5 Using White-Rot Fungus Pleurotus eryngii F032

Hadibarata

Adnan

Yusoff

et al. 2013

Water Air Soil Pollut

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The growth of white-rot fungus Pleurotus eryngii F032 in a suitable medium can degrade an azo dye Reactive Black 5 (RB5), because of its ability to produce ligninolytic enzymes such as lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase that able to degrade and transform the complex structure of the dye into a less toxic compound. The effect of environmental factors such as initial concentration of Reactive Black 5, pH, temperature of growth medium, surfactant (Tween 80), and agitation were also investigated. The productions of ligninolytic enzymes were enhanced by increasing the white-rot fungi growth in optimum conditions. The decolorization of Reactive Black 5 were analyzed by using UV-vis spectrophotometer at the maximum absorbance of 596 nm. The whiterot fungus, P. eryngii F032 culture exhibited 93.56 % decolorization of 10 mg/L RB5 within 72 h of incubation in dark condition with agitation. The optimum pH and temperature for the decolorizing activity was recorded at pH 3 and 40°C, respectively. The addition of surfactant (Tween 80) increased the decolorization to 93.57 % and agitation of growth medium at 120 rpm enhanced the distribution of nutrients to the fungus thus optimized the enzymatic reaction that resulted maximum decolorization of RB5 which was 93.57 %. The molecular docking studies were performed using Chimera visualization software as to analyze the decolorization mechanism of RB5 at molecular level.

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Section: Resultsmentioning

confidence: 99%

Microbial Decolorization of an Azo Dye Reactive Black 5 Using White-Rot Fungus Pleurotus eryngii F032

Hadibarata

Adnan

Yusoff

et al. 2013

Water Air Soil Pollut

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“…Actually several fungi have attracted a growing interest for biotreatment of wastewater with different pollutants, such as metals, inorganic nutrients and organic compounds. Important results from fungi action can be seen in recent papers (Wesenberg et al 2003;Kaushik & Malik 2009;Gao et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Secretion of lignin-degrading enzymes has been attributed to a nutrient starvation response during primary metabolism of P. chrysosporium as well as research related to the influence of lignin activity promoters (veratryl alcohol and Mn 2+ ), specifically on de-colourization treatments (Mester et al 1995;Kaushik & Malik 2009). However, some of the research results have proven to be controversial; the discrepancy is based on enzymes actuation, which is attributed to their selective specificity concerning turn of the substrate (Lee 2007).…”

Section: Introductionmentioning

confidence: 99%

Enzyme production by immobilized Phanerochaete chrysosporium using airlift reactor

et al. 2014

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Enzyme production by immobilized Phanerochaete chrysosporium was evaluated in airlift bioreactor and agitated cultures. Free mycelium and immobilized mycelium on alginate beads were tested in the decolourization of 50 and 500 mg/L of Remazol Brilliant Blue R. Dye concentration did not inhibit the fungi development in all tests. In addition, high decolourization percentage of dye was found with free mycelium (99%) in agitated flasks and with immobilized mycelium in airlift (98%). However, decolourization period by immobilized mycelium (120 h) was greater than that by the free mycelium (14 h). Important manganese peroxidase, lignine peroxidase and laccase activities were identified in decolourization process. Manganese peroxidase appeared to be promoted by high dye concentrations during the treatment with immobilized mycelium, but this enzyme was not detected with free mycelium in airlift. Bioreactor prompted also laccase and lignine peroxidase actions in both tests; free mycelium registered a maximum laccase action of 31.569 × 10 3 U/L in 70 h, whereas immobilized mycelium registered 1.680 × 10 3 U/L in 170 h, while lignine peroxidase secretion by free P. chrysosporium was higher (1.300 × 10 3 U/L) than immobilized mycelium (1.250 × 10 3 U/L). Maximum laccase activity coincided with the maximum percentage of decolourization, however, high peroxidase activity was identified from the start of dye treatment.

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“…Fungi have long been recognized for their abilities to transform a broad range of recalcitrant compounds through the use of non-specific extracellular oxidative enzymes (Gao et al 2010, Kaushik andMalik 2009). The idea of using fungi for environmental bioremediation sets its basis in the late 80's (Bumpus and Aust 1987), when a direct connection between the degradation of aromatic compounds and the fungal enzymatic pattern involved in the delignification of wood was hypothesized for the first time.…”

Section: Fungal Treatmentmentioning

confidence: 99%