Fungal endophytes promote the accumulation of Amaryllidaceae alkaloids in <scp><i>Lycoris radiata</i></scp>

Zhou, Jiayu; Liu, Zhilin; Wang, Songfeng; Li, Jie; Li, Yikui; Chen, Weikang; Ren, Wang

doi:10.1111/1462-2920.14958

Cited by 33 publications

(20 citation statements)

References 55 publications

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“…In our study, a large proportion of OTUs from the seed surfaces was also detected in macerated seeds after surface sterilization. Endophytes can also enter the seed from the environment, and thus an overlap of surface and inner seed OTUs is expected to some extent (Shahzad et al 2018;Li et al 2019;Abdullaeva et al 2020;Ozkurt et al 2020;Zhou et al 2020;Patil and Maheshwari 2021;Santos and Olivares 2021). However, we cannot exclude the possibility that the macerated samples were contaminated with surface microbiota in our study, especially those of barley seeds, which have several outer layers that decrease the efficiency of sterilization.…”

Section: Discussionmentioning

confidence: 89%

Analysis of seed-associated bacteria and fungi on staple crops using the cultivation and metagenomic approaches

et al. 2022

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One of the key factors affecting seed quality is microbial communities residing on and in the seeds. In this study, microbial populations of seeds of conventionally and organically produced wheat, barley, and maize were analyzed using two different approaches: the cultivation method and metagenomics. For cultivation, three basic media were used: DG18 (for fungi), and nutrient agar or tryptic soy agar supplemented with cycloheximide or nystatin (for bacteria). Metagenomic sequencing was performed using the Illumina MiSeq platform. A total of 452 bacterial isolates comprising 36 genera and 5 phyla and 90 fungal isolates comprising 10 genera and 3 phyla were obtained from the seed surfaces. Among bacteria, representatives from the genera Bacillus, Pantoea, Paenibacillus, and Curtobacterium predominated, and among fungi, Aspergillus predominated. A total of 142 fungal OTUs and 201 bacterial OTUs were obtained from all the samples. Proteobacteria, Firmicutes, Bacteroides, and Actinobacteria comprised most of the bacterial OTUs, and Ascomycota comprised most of the fungal OTUs. Only 3 fungal OTUs (representatives of Curvibasidium, Venturia, and Dermateaceae) were exclusively present only within seeds and not on the seed surfaces. Barley seeds had the highest microbial load and richness, whereas corn had the lowest. Wheat and barley shared a higher number of OTUs than either of them did with corn with higher overlap between conventionally grown cereals than between organically grown cereals. Some OTUs were farming specific. This study demonstrates that the microbiome of cereal seeds is greatly dependent on the species of the host and is less affected by agricultural practices.

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Section: Discussionmentioning

confidence: 89%

Analysis of seed-associated bacteria and fungi on staple crops using the cultivation and metagenomic approaches

et al. 2022

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“…Carlton in absence of phytohormone, whereas high amounts of galantamine were detected in differentiated tissues cultured in low auxin (4 mg/L of NAA) as compared to undifferentiated tissue cultured in high auxin (20 mg/L of NAA) [54]. Furthermore, in nature it was observed that plants synthesize alkaloids in response to different biotic and abiotic factors [124]. Therefore, these factors, or the associated signaling compounds produced in response to these factors, can be used as elicitors to enhance the biosynthesis of specialized metabolites in in vitro techniques [125,126].…”

Section: Biotechnological Production Of Amaryllidaceae Alkaloidsmentioning

confidence: 97%

Biosynthesis and Biological Activities of Newly Discovered Amaryllidaceae Alkaloids

Koirala

Mérindol

et al. 2020

Molecules

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Alkaloids are an important group of specialized nitrogen metabolites with a wide range of biochemical and pharmacological effects. Since the first publication on lycorine in 1877, more than 650 alkaloids have been extracted from Amaryllidaceae bulbous plants and clustered together as the Amaryllidaceae alkaloids (AAs) family. AAs are specifically remarkable for their diverse pharmaceutical properties, as exemplified by the success of galantamine used to treat the symptoms of Alzheimer’s disease. This review addresses the isolation, biological, and structure activity of AAs discovered from January 2015 to August 2020, supporting their therapeutic interest.

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“…The content of alkaloids assay was carried out by HPLC (Shimadzu, LC-20AT) on a reverse phase column (InertSustain C18, 5 µm, 4.6 mm × 250 mm) according to the method of Zhou et al [ 38 ]. The solvent composition was: phase A, 0.03% di-n-dibutylamine in H 2 O, phase B, acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in Lycoris aurea

Wang

Liu

et al. 2021

IJMS

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As a kind of Amaryllidaceae alkaloid which is accumulated in the species of Lycoris plants, lycorine has a range of physiological effects. The biosynthesis pathway of lycorine has been partly revealed, but the transport and accumulation mechanisms of lycorine have rarely been studied. In this study, an ATP-binding cassette (ABC) transporter from Lycoris aurea (L’Hér) Herb., namely LaABCB11, was cloned and functionally characterized. Heterologous expression showed that LaABCB11 transported lycorine in an outward direction, increased the tolerance of yeast cells to lycorine, and caused a lower lycorine accumulation in transformants than control or mutant in yeast. LaABCB11 is associated with the plasma membrane, and in situ hybridization indicated that LaABCB11 was mainly expressed in the phloem of leaves and bulbs, as well as in the cortical cells of roots. These findings suggest that LaABCB11 functions as a lycorine transport and it might be related to the translocation and accumulation of lycorine from the leaves and bulbs to the roots.

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Fungal endophytes promote the accumulation of Amaryllidaceae alkaloids in Lycoris radiata

Cited by 33 publications

References 55 publications

Analysis of seed-associated bacteria and fungi on staple crops using the cultivation and metagenomic approaches

Analysis of seed-associated bacteria and fungi on staple crops using the cultivation and metagenomic approaches

Biosynthesis and Biological Activities of Newly Discovered Amaryllidaceae Alkaloids

An ATP-Binding Cassette Transporter, LaABCB11, Contributes to Alkaloid Transport in Lycoris aurea

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