Fungal Extracellular Vesicles Are Involved in Intraspecies Intracellular Communication

Bitencourt, Tamires Aparecida; Hatanaka, Otavio; Pessoni, André Moreira; Freitas, Mateus Silveira; Trentin, Gabriel; Santos, Patrick Wellington da Silva; Rossi, Antônio; Martinez-Rossi, Nilce M.; Alves, Lysangela Ronalte; Casadevall, Arturo; Rodrigues, Márcio L.; Almeida, Fausto

doi:10.1128/mbio.03272-21

Cited by 35 publications

(29 citation statements)

References 70 publications

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“…From this projection, the autoregulatory function of fungal EVs in the cellular metabolism and cell communication, which reinforces the role of RNA-EVs in interkingdom and intraspecies signaling, is evident. Indeed, a recent study demonstrated the regulatory function of fungal EVs in fungal gene expression, governing the pathophysiological attributes of the pathogenic fungi C. albicans, A. fumigatus , and P. brasiliensis [ 54 ]. However, whether exonic sRNAs could be mature miRNAs exported by fungal EVs to confer faster adaptive responses requires further study and elucidation.…”

Section: Rna Content Of Fungal Evsmentioning

confidence: 99%

The RNA Content of Fungal Extracellular Vesicles: At the “Cutting-Edge” of Pathophysiology Regulation

Bitencourt

Pessoni

Oliveira

et al. 2022

Cells

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The role of extracellular vesicles (EVs) in interkingdom communication is widely accepted, and their role in intraspecies communication has been strengthened by recent research. Based on the regulation promoted by EV-associated molecules, the interactions between host and pathogens can reveal different pathways that ultimately affect infection outcomes. As a great part of the regulation is ascribable to RNA contained in EVs, many studies have focused on profiling RNAs in fungal and host EVs, tracking their accumulation during infection, and identifying potential target genes. Herein, we overview the main classes of RNA contained in fungal EVs and the biological processes regulated by these molecules, portraying a state-of-the-art picture of RNAs loaded in fungal EVs, while also raising several questions to drive future investigations. Our compiled data show unambiguously that EVs act as key elements in signaling pathways, and play a crucial role in pathosystems. A complete understanding of the processes that govern RNA content loading and trafficking, and its effect on recipient cells, will lead to improved technologies to ward off infectious agents that threaten human health.

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Section: Rna Content Of Fungal Evsmentioning

confidence: 99%

The RNA Content of Fungal Extracellular Vesicles: At the “Cutting-Edge” of Pathophysiology Regulation

Bitencourt

Pessoni

Oliveira

et al. 2022

Cells

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“…In addition, their experiments also suggested that EVs stimulated C. albicans growth, a result not exploited but consistently observed in our current work. These differences might have resulted from the use by Bitencourt et al ( 59 ) of a distinct C. albicans strain (ATCC 64548) and a different culture condition (YPD medium), which could modify the EV composition, as previously reported ( 60 ). Moreover, their experimental conditions were limited to 4 h of filamentation, when the morphological impact of EVs was just initiating in our experiments.…”

Section: Discussionmentioning

confidence: 94%

“…Altogether, these results indicate that C. albicans EVs carry a group of terpenes and fatty acids able to regulate growth and morphogenesis in C. albicans . A recent work published by Bitencourt and colleagues ( 59 ) also suggested that fungal EVs could be involved in fungal intraspecies communication and could regulate C. albicans dimorphism. However, in contrast with our results, those authors demonstrated that C. albicans EVs produced by yeasts induced filamentation, although EVs from hyphae exhibited a more prominent effect.…”

Section: Discussionmentioning

confidence: 97%

Extracellular Vesicles Regulate Biofilm Formation and Yeast-to-Hypha Differentiation in Candida albicans

Honorato

Araujo

Ellis

et al. 2022

mBio

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“…In this model, EVs have immunostimulatory activity (4) and vaccinal potential (5). Cryptococcal EVs are also required for fungal virulence (6) and intraspecies communication (7).…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicle formation inCryptococcus deuterogattiiimpacts fungal virulence and requires theNOP16gene

Castelli

Pereira

Honorato

et al. 2022

Preprint

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Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity of benzimidazoles against Cryptococcus deuterogattii. In this study, during the phenotypic characterization of C. deuterogattii mutants lacking NOP16 expression, we observed that this gene was required for EV production. Analysis of the small molecule composition of EVs produced by wild-type cells and two independent nop16Δ mutants revealed that the deletion of NOP16 resulted not only in a reduced number of EVs but also an altered small molecule composition. In a Galleria mellonella model of infection, the nop16Δ mutants were hypovirulent. The hypovirulent phenotype was reverted when EVs produced by wild-type cells, but not mutant EVs, were co-injected with the nop16Δ cells in G. mellonella. These results reveal a role for NOP16 in EV biogenesis and cargo, and also indicate that the composition of EVs is determinant for cryptococcal virulence.

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Fungal Extracellular Vesicles Are Involved in Intraspecies Intracellular Communication

Abstract: Here, we report a study about extracellular vesicles (EVs) as communication mediators in fungi. Our results demonstrated the role of EVs from Candida albicans , Aspergillus fumigatus , and Paracoccidioides brasiliensis regulating the expression of target genes and phenotype features.

Cited by 35 publications

References 70 publications

The RNA Content of Fungal Extracellular Vesicles: At the “Cutting-Edge” of Pathophysiology Regulation

The RNA Content of Fungal Extracellular Vesicles: At the “Cutting-Edge” of Pathophysiology Regulation

Extracellular Vesicles Regulate Biofilm Formation and Yeast-to-Hypha Differentiation in Candida albicans

Extracellular vesicle formation inCryptococcus deuterogattiiimpacts fungal virulence and requires theNOP16gene

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