Fungal Flora Contaminating Egyptian Ras Cheese with Reference to Their Toxins and Enzymes

Seddek, Nermien H.; Gomah, Nanis H.; Osman, Dina M.

doi:10.13189/fst.2016.040403

Cited by 10 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolated mold stains in the study were in agreement with those obtained by other researchers; Elbagory et al [66] and Seddek et al [67] who showed that Aspergillus was the most predominant isolated mold from Ras cheese and represented by Aspergillus flavus, Aspergillus niger, Aspergillus ustus, and Aspergillus fumigatus. Another study conducted by Mohamed et al [57] who showed that the most prevalent mold isolates from processed and Ras cheeses were Penicillium followed by Aspergillus.…”

Section: Total Mold Count In the Tested Samplessupporting

confidence: 92%

Assessment of aflatoxin M1 and B1 in some dairy products with referring to the analytical performances of enzyme-linked immunosorbent assay in comparison to high-performance liquid chromatography

Esam

Hafez

Khafaga³

et al. 2022

Vet World

View full text Add to dashboard Cite

Background and Aim: Aflatoxin M1 (AFM1) is a major fungal metabolite found in milk coming from aflatoxin B1 (AFB1) contaminated rations and is subsequently present in milk-based products demonstrating a serious public health hazard. This study aimed to investigate the levels of AFM1 and AFB1 in milk and some dairy products consumed widely by infants and children. Materials and Methods: This study investigated the incidence of AFM1 in 105 samples of processed cheese, Ras cheese, and raw milk (35 of each) retailed in the Egyptian markets. The degree of sensitivity and accuracy was evaluated using the enzyme-linked immunosorbent assay (ELISA) method followed by the estimation of the positive samples using the high-performance liquid chromatography (HPLC) with fluorescence detection. Mold count was determined in the examined samples by investigating AFB1 content using HPLC. Results: AFM1 was found in all investigated Ras cheese, raw milk, and 82.86% of the processed cheese samples with mean values of 51.05±6.19, 40.27±3.996, and 10.77±1.39 ng/kg, respectively. Moreover, there was statistically no significant difference between AFM1 levels in the core and crust parts of the tested Ras cheese. AFM1 contaminated Ras cheese and raw milk samples were 48.57% and 25.71%, which exceeded the European and Egyptian tolerance levels. Results showed an acceptable correlation between ELISA and HPLC methods with no significant difference (p>0.05). Alternatively, none of the examined samples proved to be contaminated with AFB1 despite the presence of mold with mean counts of 3.79±3.29, 4.39±4.34, and 4.84±4.29 log CFU/g in the examined processed cheese, Ras cheese, and raw milk samples, respectively. Conclusion: Therefore, it is urgent to regularly inspect the contamination of animal feeds with AFB1 and apply special measures and novel techniques to protect the feed and food from public health hazards.

show abstract

Section: Total Mold Count In the Tested Samplessupporting

confidence: 92%

Assessment of aflatoxin M1 and B1 in some dairy products with referring to the analytical performances of enzyme-linked immunosorbent assay in comparison to high-performance liquid chromatography

Esam

Hafez

Khafaga³

et al. 2022

Vet World

View full text Add to dashboard Cite

show abstract

“…The mycological examination of Ras cheese samples detected A. fumigatus in a percentage of 22.11% (Table 1), this result is higher than that mentioned by Elramly et al, (2019), and nearly similar to that reported by Seddek et al, (2016). The presence of A. fumigatus in Ras cheese may be related mostly to the long storage period in wood shelves, which lasts for several months for pungent flavor and texture development.…”

Section: Discussionsupporting

confidence: 70%

The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients

Hassanien

Shaker

Elsherif

2021

SVU-International Journal of Veterinary Sciences

View full text Add to dashboard Cite

This work was designed to study the incidence of Aspergillus fumigatus (A. fumigatus) in Ras cheese sold in different markets at Sohag city, Egypt and also in sputum samples of respiratory diseases patients admitted to Sohag University Hospital, as well as study the effect of silver (SNPs) and chitosan (CNPs) nanoparticles against the A. fumigatus isolates. Potato dextrose agar (PDA) was used for mycological culture of samples and the antifungal effect of nanoparticles (with different concentrations) using well diffusion method. High resolution transmission electron microscope (HRTEM) was used for detection of nanoparticles size, and scanning electron microscope (SEM) was used for investigation of nanoparticles effect on A.fumigatus morphology. The isolation rate of A. fumigatus was 22.11 % (21/95), 32.17 % (37/115) from Ras cheese and sputum samples, respectively. SNPs had inhibitory effect at concentrations 1 and 2 µg/ml, and complete growth inhibition with concentrations 4, 8, and 16 µg/ ml. While CNPs had no effect in concentrations 1 and 2 µg/ ml, but at concentrations 4, 8 and 16 µg/ ml represented inhibitory effect with mean inhibition zones 5±0.21, 9±0.41 mm and 11 ±0.77mm, respectively. SNPs and CNPs by SEM patently damaged the A. fumigatus cell structure. Using nanoparticles against A. fumigatus from food and human sources is important to stop food spoilage and protect human health. So, further studies are necessary for the investigation of suitable application methods, and the expected hazards which may occur after nanoparticles usage.

show abstract

“…Moubasher et al (2018b) isolated Aspergillus sp. from Ras cheese, the most common species isolated from surface and core of examined Ras cheese samples were; A. flavus (Zommara and Rashed 2004;ELbagory et al 2014;El-fadaly et al 2015a, b;El-Badry and Raslan 2016;Seddek et al 2016;Elramly et al 2019;Ahmed et al 2020), A. niger (ELbagory et al 2014El-fadaly et al 2015a, b;El-Badry and Raslan 2016;Seddek et al 2016;Younis et al 2016;Elramly et al 2019;Ahmed et al 2020) andA. fumigatus (ELbagory et al 2014;Seddek et al 2016;Younis et al 2016;Elramly et al 2019;Ahmed et al 2020).…”

Section: Aspergillus In Egyptmentioning

confidence: 99%

“…glaucus (El-fadaly et al 2015a, b), A. nidulans (El-fadaly et al 2015a, b;Elramly et al 2019), A. ochraceus (El-fadaly et al 2015aEl-Badry and Raslan 2016;Elramly et al 2019;Ahmed et al 2020), A. oryzae (El-fadaly et al 2015aAhmed et al 2020), A. parasiticus (Zommara and Rashed 2004;El-Badry and Raslan 2016;Younis et al 2016;Ahmed et al 2020), A. sydowii (Ahmed et al 2020), A. terreus (ELbagory et al 2014Embaby et al 2015;Younis et al 2016;Elramly et al 2019) and A. ustus (Seddek et al 2016). Furthermore, Hegazy et al (2014) reported isolation of A.…”

Section: Aspergillus In Egyptmentioning

confidence: 99%

The Egyptian Ascomycota 1: Genus Aspergillus

Abdel-Azeem

Abu-Elsaoud

Darwish

et al. 2020

MBJ

View full text Add to dashboard Cite

Since Pier Antonio Micheli described and published genus Aspergillus in Nova Plantarum Genera in 1729 the genus attracted an immense interest. The published Egyptian literature on the genus is scattered and fragmentary. By screening the available sources of information since 1921, it was possible to figure out a range of 150 taxa that could be representing genus Aspergillus in Egypt up to the present time. Ten species of Aspergillus were introduced as type materials from Egypt since 1964 till now. Recorded taxa were assigned to 5 subgenera and 25 sections. This article includes Aspergillus species that are known to Egypt, provides a comprehensive checklist of species isolated from Egypt and provisional key to the identification of reported taxa is given. Although the present study will add some new data to our information concerning the Ascomycota of Egypt, this work must be considered as a provisional one always waiting for continuous supplementation.

show abstract

Fungal Flora Contaminating Egyptian Ras Cheese with Reference to Their Toxins and Enzymes

Cited by 10 publications

References 18 publications

Assessment of aflatoxin M1 and B1 in some dairy products with referring to the analytical performances of enzyme-linked immunosorbent assay in comparison to high-performance liquid chromatography

Assessment of aflatoxin M1 and B1 in some dairy products with referring to the analytical performances of enzyme-linked immunosorbent assay in comparison to high-performance liquid chromatography

The impact of silver and chitosan nanoparticles on A. fumigatus isolates from Ras cheese and sputum of respiratory patients

The Egyptian Ascomycota 1: Genus Aspergillus

Contact Info

Product

Resources

About