Fungal immunomodulatory proteins: characteristic, potential antitumor activities and their molecular mechanisms

Li, Qizhang; Zheng, Yu-Zhang; Zhou, Xuan‐Wei

doi:10.1016/j.drudis.2018.09.014

Cited by 50 publications

(30 citation statements)

References 51 publications

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“…These compounds display immunomodulating and anti-infective effects [125][126][127]. Many species of mushrooms have been found to produce immunomodulators, such as Agaricus bisporus, Agaricus blazei, Amanita pantherina, Boletus satanas, Coprinus cinereus, Cordyceps sinensis, Ga. lucidum, Grifola frondosa, Flammulina velutipes, Ischnoderma resinosum, Lactarius deterrimus, Laetiporus sulphureus, Lentinus tigrinus, Trametes versicolor, and Volvariella volvacea [115,[128][129][130], as is detailed in Figure 5 and Table 3.…”

Section: Potential Of Fungal Bioactive Compounds For Immunomodulatorsmentioning

confidence: 99%

“…Ganoderma applanatum Activating NK cell [128] Ganolucidoid A and B Ganoderma lucidum NO production, anti-inflammatory activities [130] Lanostane Hypholoma fasciculare NO production, anti-inflammatory activities [151] The fungal immunomodulatory protein FIP-fve that was obtained from Flammulina velutipes has been employed to suppress the respiratory syncytial virus (RSV), which is known to cause bronchiolitis. FIP-fve effectively decreased RSV replication, IL-6 expression, and inflammation via inhibition of NF-κB translocation and respiratory pathogenesis in RSV-challenged mice.…”

Section: Exobiopolymersmentioning

confidence: 99%

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Natural Bioactive Compounds from Fungi as Potential Candidates for Protease Inhibitors and Immunomodulators to Apply for Coronaviruses

et al. 2020

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The inhibition of viral protease is an important target in antiviral drug discovery and development. To date, protease inhibitor drugs, especially HIV-1 protease inhibitors, have been available for human clinical use in the treatment of coronaviruses. However, these drugs can have adverse side effects and they can become ineffective due to eventual drug resistance. Thus, the search for natural bioactive compounds that were obtained from bio-resources that exert inhibitory capabilities against HIV-1 protease activity is of great interest. Fungi are a source of natural bioactive compounds that offer therapeutic potential in the prevention of viral diseases and for the improvement of human immunomodulation. Here, we made a brief review of the current findings on fungi as producers of protease inhibitors and studies on the relevant candidate fungal bioactive compounds that can offer immunomodulatory activities as potential therapeutic agents of coronaviruses in the future.

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Section: Potential Of Fungal Bioactive Compounds For Immunomodulatorsmentioning

confidence: 99%

Section: Exobiopolymersmentioning

confidence: 99%

Natural Bioactive Compounds from Fungi as Potential Candidates for Protease Inhibitors and Immunomodulators to Apply for Coronaviruses

et al. 2020

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“…At present, more than twenty FIPs have been isolated or synthesized since the first FIP, LingZhi-8 (LZ-8 or FIP-glu), was isolated in 1989 (26). The FIPs from different origins not only share high homology in nucleotides and amino acid sequences, but also show significant similarity in immunomodulation and anti-tumor activities (6,42).…”

Section: Discussionmentioning

confidence: 99%

“…BLAST results suggested that more than sixteen FIPs share a Fve conserved domain. The Fve protein family showed significant immunomodulatory activity associated with many biologic processes (15,42). Noticeably, there are an exception in the reported FIPs, ACA, YZP, and c13717 ( Supplementary Table S1), which share low identities (<10%) to those FIPs from the Fve family.…”

Section: Discussionmentioning

confidence: 99%

“…FIPs are considered as a T cell activator which can stimulate several important cytokines and molecular factors (IL-2, IL-3, IFN-g, and TNF-a etc. ), and can inhibit progression or migration of several kinds of cancer cells (15,42). However, homologous FIPs from different sources could display diverse activities and anti-tumor mechanisms, for example, FIP-gts from Ganoderma tsugae induces premature senescence of A549 lung cancer cells, while LZ-8 from Ganoderma lucidum increases A549 G1 arrest, and FIP-sch3 (FIP from Stachybotrys chartarum) induces A549 apoptosis and inhibits migration (36,43,44).…”

Section: Introductionmentioning

confidence: 99%

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Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH

Sun

Deng

et al. 2020

Front. Immunol.

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A novel fungal immunomodulatory protein (FIP) was found in the precious medical and edible mushroom Morchella conica SH, defined as FIP-mco, which belongs to the FIP family. Phylogenetic analyses of FIPs from different origins were performed using Neighbor-Joining method. It was found that FIP-mco belonged to a new branch of the FIP family and may evolved from a different ancestor compared with most other FIPs. The cDNA sequence of FIP-mco was cloned and expressed in the yeast Pichia Pastoris X33. The recombinant protein of FIP-mco (rFIP-mco) was purified by agarose Ni chromatography and determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The protein rFIP-mco could significantly suppress the proliferation of A549 and HepG2 cells at the concentration of 15 and 5 mg/ml, respectively, and inhibited the migration and invasion of human A549 and HepG2 cells at the concentration of 15 and 30 mg/ml respectively in vitro. Further, rFIPmco can significantly reduce the expression levels of TNF-a, IL-1b, and IL-6 in the THP1 cells (human myeloid leukemia mononuclear cells). In order to explore the potential mechanism of the cytotoxicity effect of rFIP-mco on A549 and HepG2 cells, cell cycle and apoptosis assay in the two cancer cells were conducted. The results demonstrated that G0/G1 to S-phase arrest and increased apoptosis may contribute to the proliferation inhibition by rFIP-mco in the two cancer cells. Molecular mechanism of rFIP-mco's reduction effect on the inflammatory cytokines was also studied by suppression of the NF-kB signaling pathway. It showed that suppression of NF-kB signaling is responsible for the reduction of inflammatory cytokines by rFIP-mco. The results indicated the prospect of FIP-mco from M. conica SH as an effective and feasible source for cancer therapeutic studies and medical applications.

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Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in RAW264.7 cells

Chang

et al. 2019

Journal Cellular Physiology

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Ganoderma lucidum immunomodulatory protein (FIP-glu) is an active ingredient with potential immunoregulatory functions. The study was conducted to explore the immunomodulatory activities of recombinant FIP-glu (rFIP-glu) and its possible mechanism in macrophage RAW264.7 cells. In vitro assays of biological activity indicated that rFIP-glu significantly activated RAW264.7 cells and possessed proinflammatory and anti-inflammatory abilities. RNA sequencing analysis and Western blot analysis showed that macrophage activation involved PI3K/Akt and MAPK pathways. Furthermore, realtime quantitative polymerase chain reaction indicated that the PI3K inhibitor LY294002 blocked the messenger RNA (mRNA) levels of MCP-1 (CCL-2), the MEK1/2 inhibitor U0126 reduced the mRNA levels of TNF-α and MCP-1 (CCL-2), and the JNK1/2/3 inhibitor SP600125 prevented the upregulation of inducible nitric oxide synthase mRNA in rFIP-glu-induced cells. rFIP-glu did not mediate these inflammatory effects through a general pathway but rather through a different pathway for a different inflammatory mediator. These data imply that rFIP-glu possessed immunomodulatory activity in macrophages, which was mediated through PI3K/Akt and MAPK pathways.macrophage activation, mitogen-activated protein kinases, phosphatidylinositol 3-kinases, RAW 264.7 cells, sequence analysis, RNA

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Fungal immunomodulatory proteins: characteristic, potential antitumor activities and their molecular mechanisms

Cited by 50 publications

References 51 publications

Natural Bioactive Compounds from Fungi as Potential Candidates for Protease Inhibitors and Immunomodulators to Apply for Coronaviruses

Natural Bioactive Compounds from Fungi as Potential Candidates for Protease Inhibitors and Immunomodulators to Apply for Coronaviruses

Identification and Functional Characterization of a Novel Immunomodulatory Protein From Morchella conica SH

Immunomodulatory activity of Ganoderma lucidum immunomodulatory protein via PI3K/Akt and MAPK signaling pathways in RAW264.7 cells

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