Fungal immunomodulatory proteins in the context of biomedicine

Lopez-Gartner, German Ariel

doi:10.2741/e803

Cited by 10 publications

(3 citation statements)

References 98 publications

(155 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies have shown that FIP possess broad spectrum immunomodulatory and anticancer activities, suggesting that they may be promising candidates in clinical disease therapies or for treatment as adjuvant agents 4,17 . FIP can be orally consumed and have high stability when exposed to denaturing conditions 18,19 .…”

Section: Discussionmentioning

confidence: 99%

“…Several experiments showed that FIP is a potent T cell activator that enhances the immune response by modulating the production of important cytokines and molecular factors (IL-2, IFN-γ, IL-1β, TNF-α etc.) in several vertebrate model systems 4 . The ability of these cytokines to produce a tumour suppressing environment indirectly proves that FIP regulates the immune system and has a cytotoxic effect on tumour cells 5 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation

Wang

Gao

Bai

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Fungal immunoregulatory proteins (FIP) are effective small molecule proteins with broad-spectrum immunomodulatory and anti-cancer activities and can be potential agents for the development of clinical drugs and health food additives. In this study, a new member of FIP named FIP-bbo was obtained through Botryobasidium botryosum genome mining. FIP-bbo has the typical characteristics of FIP but is genetically distant from other FIPs. Recombinant FIP-bbo (rFIP-bbo) was produced in an optimized E. coli expression system, and the pure protein was isolated using a Ni-NTA column. Antineoplastic experiments suggested that FIP-bbo is similar to LZ-8 in inhibiting various cancer cells (Hela, Spac-1, and A549) at lower concentrations, but it is not as potent as LZ-8. The molecular mechanism by which FIP-bbo, FIP-fve, and LZ-8 are cytotoxic to cancer cells has been discussed based on molecular dynamics simulation. Point mutations that may improve the thermal stability of FIP-fve and FIP-bbo were predicted. These results not only present a new candidate protein for the development of anticancer adjuvants, but also provide an approach for designing FIPs with high anticancer activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation

Wang

Gao

Bai

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Fungal immunomodulatory proteins (FIPs) are a new class of bioactive proteins reported in fungi, including those popular edible and medicinal mushrooms on the market, such as G. lucidum (LZ-8 or FIP-glu) [ 10 ], F. velutipes (FIP-fve) [ 11 ], V. volvacea (FIP-vvo) [ 12 ], and G. sinensis (FIP-gsi) [ 13 ]. The bioactive effects of FIPs have attracted research interest since their discovery, such as hemagglutination, anti-allergy, anti-inflammation, immunomodulation, and anti-tumor [ 14 , 15 ]. The immune modulators originating from mushrooms stimulate both the innate immune system and the acquired immune system [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Wang

et al. 2023

Molecules

View full text Add to dashboard Cite

A novel fungal immunomodulatory protein (FIP), identified as FIP-hma, was discovered in the genome of an edible mushroom Hypsizygus marmoreus. Bioinformatics analysis suggested FIP-hma contained the cerato-platanin (CP) conserved domain and was categorized into Cerato-type FIP. In phylogenetic analysis, FIP-hma was clustered into a new branch of the FIP family, displaying large system divergence from most of the other FIPs. The higher gene expression of FIP-hma was observed during the vegetative growth stages than that during the reproductive growth stages. In addition, the cDNA sequence of FIP-hma was cloned and successfully expressed in Escherichia coli (E. coli) BL21(DE3). The recombinant protein of FIP-hma (rFIP-hma) was neatly purified and isolated by Ni-NTA and SUMO-Protease. The iNOS, IL-6, IL-1β, and TNF-α levels of RAW 264.7 macrophages were upregulated by rFIP-hma, indicating its activation of an immune response by regulating central cytokines. No cytotoxic effects were observed in an MTT test. The findings of this work discovered a novel immunoregulatory protein from H. marmoreus, provided a systematic bioinformatic profile, suggested an effective approach for its heterologous recombinant production, and reported its potent immunoregulatory activity in macrophages. This study sheds light on the physiological function research of FIPs and their further industrial utilization.

show abstract

Functional evaluation of a recombinant fungal immunomodulatory protein from L. rhinocerus produced in P. pastoris and E. coli host expression systems

Ejike

Chan

Lim

et al. 2021

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Fungal immunomodulatory proteins in the context of biomedicine

Cited by 10 publications

References 98 publications

Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation

Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium botryosum and Protein Structure Analysis using Molecular Dynamic Simulation

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Functional evaluation of a recombinant fungal immunomodulatory protein from L. rhinocerus produced in P. pastoris and E. coli host expression systems

Contact Info

Product

Resources

About