Fungal infection of sutureless self-sealing incision for cataract surgery

Garg, Prashant; Mahesh, Sankaranarayana P.; Bansal, Akash; Gopinathan, Usha; Rao, Gullapalli N

doi:10.1016/s0161-6420(03)00861-3

Cited by 59 publications

(61 citation statements)

References 19 publications

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“…Hyphae are almost always parallel to the corneal surface; perpendicular hyphae were found in experimental studies in rabbits subjected to intense corticosteroid use (31) . Figure 3A shows a mycetoma in the incision site for a previous transplant; this confirms the hypothesis by Garg et al of contamination of the incision tunnel for cataract surgery with sutureless, self-adhesive material (32) . Xie et al recommend penetrating keratoplasty for fungal ulcers of difficult treatment (25) , which is reasonable, as we confirmed cure in four patients after the second transplant; however, in two others cases there was no confirmed cure, although they have not been submitted to other transplants.…”

Section: Discussionsupporting

confidence: 85%

Reflexões sobre a ceratite fúngica por meio dos achados de exames histopatológicos

Monte

Stadtherr

2013

Rev. bras.oftalmol.

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Purpose: The study of fungal invasion and pathogenicity in corneal tissue observed through the histopathological examination of specimens obtained through penetrating keratoplasty ('PKP') of samples obtained from an Eye Bank ('EB'), with the aim of applying findings in diagnosis and treatment of the condition (n=35), a faixa etária acima de 60 anos a mais numerosa com 1/3 dos pacientes. Os casos de ceratite fúngica correspondiam à média de 6,4% (n=597) do material recebido no BOO e 1,65% (n=2310) dos transplantes ocorrido com o material fornecido nos últimos 5 anos. Pelas informações dos cirurgiões 39,5% (n=38) dos casos deviam-se a perfuração corneana. Usando as datas dos transplantes foi feita uma Tábua de Observação. Em 11 (n=38) casos, a córnea procedia de transplante anterior. As formas leveduriformes nos tecidos corneanos eram de 63% (n=38). Em 50% (n=38) dos casos o infiltrado inflamatório era pequeno ou inexistente. A camada de Descemet estava íntegra em 13% (n=38), enquanto eram encontrados fungos na superfície corneana de 45% (n=38) dos casos. Conclusão: A coleta do material poderá ser feita com sucesso mesmo depois de instalado o tratamento, entretanto, nas úlceras de córnea deve ser feito preferentemente a coleta de material com espátula para exame laboratorial e a microscopia confocal in vivo. A predominância das leveduras poderá ser devido a alterações morfológicas do fungo sofridas no tecido corneano. A penetração intraocular é facilitada por alterações da Camada de Descemet e pela própria capacidade do fungo de penetrar nos tecidos justificando o tratamento sistêmico desde o início. Descritores: Ceratite/diagnóstico; Ceratite/patologia; Infecções oculares fúngicas/diagnósticoThe authors declare no conflicts of interest

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Section: Discussionsupporting

confidence: 85%

Reflexões sobre a ceratite fúngica por meio dos achados de exames histopatológicos

Monte

Stadtherr

2013

Rev. bras.oftalmol.

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“…Furthermore, A. terreus has been reported as a human pathogen and can cause superficial, cutaneous, and subcutaneous mycoses that affect the nail bed (28), outer ear canal (60), and skin (16). More recently, A. terreus has been associated with postoperative osteomyelitis (43), endophthalmitis (22), and peritonitis (63), and the species has been identified as an emerging opportunistic pathogen in immunocompromised populations (3,4). Knowledge of A. terreus pathogenesis is limited; however, the species is thermotolerant and produces aleurioconidia that may provide an advantage in dissemination (48).…”

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confidence: 99%

Monoclonal Antibodies to Hyphal Exoantigens Derived from the Opportunistic Pathogen Aspergillus terreus

Nayak

Green

Janotka

et al. 2011

Clin. Vaccine Immunol.

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Aspergillus terreus has been difficult to identify in cases of aspergillosis, and clinical identification has been restricted to the broad identification of aspergillosis lesions in affected organs or the detection of fungal carbohydrates. As a result, there is a clinical need to identify species-specific biomarkers that can be used to detect invasive A. terreus disease. Monoclonal antibodies (MAbs) were developed to a partially purified preparation of cytolytic hyphal exoantigens (HEA) derived from A. terreus culture supernatant (CSN). Twentythree IgG1 isotype murine MAbs were developed and tested for cross-reactivity against hyphal extracts of 54 fungal species. Sixteen MAbs were shown to be specific for A. terreus. HEA were detected in conidia, hyphae, and in CSN of A. terreus. HEA were expressed in high levels in the hyphae during early stages of A. terreus growth at 37°C, whereas at room temperature the expression of HEA peaked by days 4 to 5. Expression kinetics of HEA in CSN showed a lag, with peak levels at later time points at room temperature and 37°C than in hyphal extracts. Serum spiking experiments demonstrated that human serum components do not inhibit detection of the HEA epitopes by MAb enzyme-linked immunosorbent assay (ELISA). Immunoprecipitation and proteomic analysis demonstrated that MAbs 13E11 and 12C4 immunoprecipitated a putative uncharacterized leucine aminopeptidase (Q0CAZ7), while MAb 19B2 recognized a putative dipeptidyl-peptidase V (DPP5). Studies using confocal laser scanning microscopy showed that the uncharacterized leucine aminopeptidase mostly localized to extracellular matrix structures while dipeptidyl-peptidase V was mostly confined to the cytoplasm.

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“…Siempre que se sospeche una lesión de etiología infecciosa a nivel corneal se deben separar las muestras para realizar cultivos bacterianos y micóticos, estos últimos de gran importancia si existen antecedentes que hagan sospechar una queratitis fúngica (tabla 3) (20)(21)(22).…”

Section: Carlos Pérez Díaz Patricia Barrios Castañeda José David MIunclassified

Colección corneal intraestromal y queratitis por Fusarium Spp: presentación de dos casos y revisión de la literatura

Díaz

Castañeda

Echavarría³

et al. 2013

Rev. Med.

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ResumenLas infecciones oculares por hongos filamentosos han aumentado su incidencia con el pasar de los años. Los traumas corneales, la terapia inmunosupresora y el uso de lentes de contacto han hecho que éstas aumenten su incidencia, pero existe un pequeño porcentaje de paciente que sin ningún factor de riesgo presentan infecciones por estos mohos. En este artículo reportamos dos casos de infección ocular por Fusarium spp. en dos pacientes de 21 y 30 años de edad respectivamente, inmunocompetentes, pertenecientes a las Fuerzas Militares y sin factores de riesgo asociados.Palabras Clave: Queratitis fúngica, moho, Fusarium spp. COLLECTION INTRASTROMAL CORNEAL AND KERATITIS BY FUSARIUM SPP: REPORT OF TWO CASES AND REVIEW OF THE LITERATURE AbstractThe filamentous fungal eye infections have been increasing in incidence in the last years. Corneal trauma, immunosuppressive therapy and use of contact lenses have increased their incidence, nonetheless a small percentage of patients with no risk factors still present with these mold infections. In this article we report a case of ocular infection by Fusarium spp. in two patients, a 21-year-old and a 30-year-old males. They work with the Military Forces, and were immunocompetent with no risk factors associated to the problem.

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Fungal infection of sutureless self-sealing incision for cataract surgery

Cited by 59 publications

References 19 publications

Reflexões sobre a ceratite fúngica por meio dos achados de exames histopatológicos

Reflexões sobre a ceratite fúngica por meio dos achados de exames histopatológicos

Monoclonal Antibodies to Hyphal Exoantigens Derived from the Opportunistic Pathogen Aspergillus terreus

Colección corneal intraestromal y queratitis por Fusarium Spp: presentación de dos casos y revisión de la literatura

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