Fungal Lipases: Insights into Molecular Structures and Biotechnological Applications in Medicine and Dairy Industry

Darwish, A. A.; Nahas, Hebatallah H. Abo; Korra, Yasmin H.; Osman, Alaa A.; El-Kholy, Wedad M.; Reyes-Córdova, Maria; Saied, Essa M.; Abdel-Azeem, Ahmed M.

doi:10.1007/978-3-030-85603-8_13

Cited by 3 publications

(3 citation statements)

References 190 publications

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“…Higher lipase yields might result from extending the fermentation time or putting continuous culture systems in place. Optimizing the extraction and purification processes could result in a higher overall lipase recovery during downstream processing [31]. The collective efforts of researchers in this field are essential for meeting the growing demands for lipase enzymes in various industrial applications [32,33].…”

Section: Resultsmentioning

confidence: 99%

Evaluation and Characterization of Lipase Production in Aspergillus niger Isolate Qurna for Biotechnological Applications

Kadhim,

Ali,

Al-Hawash

et al. 2023

IJDNE

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This study aims to investigate the lipase-producing capabilities of the isolated strain Aspergillus niger Qurna and to provide an analysis of contemporary scholarly contributions on the subject. Our objective is to offer a comprehensive survey of recent advancements in lipase production from Aspergillus niger, juxtaposing our findings with those from other relevant studies. Significant lipase activity was observed within the Aspergillus niger Qurna isolate, with a precipitation area exceeding 55 mm by the fifth day of tracking lipase activity via a chromogenic method. The variation in the precipitation zone size among different Aspergillus niger isolates might be attributable to genetic differences or environmental influences on gene expression. Upon comparing the Aspergillus niger Qurna isolate with international isolates, it was determined that the local Aspergillus niger isolate exhibited superior lipase activity. This makes it a promising candidate for biotechnological applications, including its potential use in food, pharmaceutical, detergent, and chemical industries. Therefore, the identification of Aspergillus niger strains with high lipase activity holds significant importance for biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Evaluation and Characterization of Lipase Production in Aspergillus niger Isolate Qurna for Biotechnological Applications

Kadhim,

Ali,

Al-Hawash

et al. 2023

IJDNE

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“…The secretion of lipases by entomopathogenic fungi is essential for the degradation of the cuticle and contributes to its virulence and pathogenicity [ 25 ]. Fungi are considered the best source to produce lipases with advantages in ease of fermentation and low-cost extraction methods [ 37 ]. In this research, lipases were detected at low levels in all tested isolates, except for the isolate T. harzianum Th180, and it was the lowest enzymatic activity in the co-production of enzymes in submerged fermentation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Fungal Lytic Enzymatic Extracts Produced Under Submerged Fermentation as Enhancers of Entomopathogens’ Biological Activity

Mejía,

Bautista,

García

et al. 2024

Curr Microbiol

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The application of enzymes in agricultural fields has been little explored. One potential application of fungal lytic enzymes (chitinases, lipases, and proteases) is as an additive to current biopesticides to increase their efficacy and reduce the time of mortality. For this, a screening of lytic overproducer fungi under submerged fermentation with a chemical-defined medium was performed. Then, the enzymatic crude extract (ECE) was concentrated and partially characterized. This characterization consisted of measuring the enzymatic activity (lipase, protease and, chitinase) and determining the enzyme stability after storage at temperatures of − 80, − 20 and, 4 °C. And lastly, the application of these concentrated enzymatic crude extracts (C-ECE) as an enhancer of spores-based fungal biopesticide was proven. Beauveria were not as good producers of lytic enzymes as the strains from Trichoderma and Metarhizium. The isolate M. robertsii Mt015 was selected for the co-production of chitinases and proteases; and the isolate T. harzianum Th180 for co-production of chitinases, lipases, and proteases. The C-ECE of Mt015 had a protease activity of 18.6 ± 1.1 U ml−1, chitinase activity of 0.28 ± 0.01 U ml−1, and no lipase activity. Meanwhile, the C-ECE of Th180 reached a chitinase activity of 0.75 U ml−1, lipase activity of 0.32 U ml−1, and protease activity of 0.24 U ml−1. Finally, an enhancing effect of the enzymatic extracts of M. robertsii (66.7%) and T. harzianum (43.5%) on the efficacy of B. bassiana Bv064 against Diatraea saccharalis larvae was observed. This work demonstrates the non-species-specific enhancing effect of enzymatic extracts on the insecticidal activity of conidial-based biopesticides, which constitutes a contribution to the improvement of biological control agents’ performance.

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“…Lipases are versatile enzymes with a wide range of catalytic properties that make them suitable for industrial processes related to food (dairy, fat and oil, bakery, cheese flavoring, wine, meat and fish), pharmaceuticals and medicine, cosmetics, textiles, and detergents [ 32 ]. Additionally, lipases have applications in agriculture (for example, CalB lipase is used to produce the herbicide dimethenamide-P catalyzing enantioselective transamination), environmental cleaning (bioremediation, the paper industry, leather degreasing, and plastic biodegradation), bioenergy and biodiesel production, and as enzyme biosensors [ 32 , 33 , 34 ]. Nowadays, the main sources of lipases are microorganisms: bacteria and fungi.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi

Gutiérrez-Domínguez

Chí-Manzanero

Rodríguez-Argüello

et al. 2022

IJMS

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Lipases are enzymes that hydrolyze triglycerides to fatty acids and glycerol. A typical element in lipases is a conserved motif of five amino acids (the pentapeptide), most commonly G-X-S-X-G. Lipases with the pentapeptide A-X-S-X-G are present in species of Bacillus, Paucimonas lemoignei, and the yeast Trichosporon asahii; they are usually thermotolerant and solvent resistant. Recently, while searching for true lipases in the Trichoderma harzianum genome, one lipase containing the pentapeptide AHSMG was identified. In this study, we cloned from T. harzianum strain B13-1 the lipase ID135964, renamed here as ThaL, which is 97.65% identical with the reference. We found that ThaL is a lid-containing true lipase of cluster III that belongs to a large family comprising highly conserved proteins in filamentous fungi in the orders Hypocreales and Glomerellales, in which predominantly pathogenic fungi are found. ThaL was expressed in conidia, as well as in T. harzianum mycelium, where it was cultured in liquid minimal medium. These results—together with the amino acid composition, absence of a signal peptide, mitochondrial sorting prediction, disordered regions in the protein, and lineage-specific phylogenetic distribution of its homologs—suggest that ThaL is a non-canonical effector. In summary, AHSMG-lipase is a novel lipase family in filamentous fungi, and is probably involved in pathogenicity.

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Fungal Lipases: Insights into Molecular Structures and Biotechnological Applications in Medicine and Dairy Industry

Cited by 3 publications

References 190 publications

Evaluation and Characterization of Lipase Production in Aspergillus niger Isolate Qurna for Biotechnological Applications

Evaluation and Characterization of Lipase Production in Aspergillus niger Isolate Qurna for Biotechnological Applications

Assessment of Fungal Lytic Enzymatic Extracts Produced Under Submerged Fermentation as Enhancers of Entomopathogens’ Biological Activity

Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi

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