Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses

Liu, Lijiang; Xiè, Jiǎtāo; Chéng, Jiāsēn; Fù, Yànpíng; Li, Guoqing; Yi, Xianhong; Jiāng, Dàohóng

doi:10.1073/pnas.1401786111

Cited by 191 publications

(160 citation statements)

References 42 publications

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“…However, the diseases caused by S. sclerotiorum have not been adequately controlled by conventional technologies thus far (4,5). A number of mycoviruses have been molecularly characterized and associated with attenuation of the virulence of S. sclerotiorum, including a DNA virus (6, 7), a negative-sense RNA virus (8), mitoviruses (9,10), and double-stranded RNA (dsRNA) (11) and single-stranded positive-sense RNA [ss(ϩ)RNA)] viruses (12)(13)(14)(15)(16)(17). Given the observations that mycoviruses transmitted intercellularly through hyphal anastomosis and extracellularly via viral particles can induce hypovirulence in S. sclerotiorum (7,18), mycoviruses offer a promising approach to reduce crop damage caused by the fungus.…”

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Transfection of Sclerotinia sclerotiorum with In Vitro Transcripts of a Naturally Occurring Interspecific Recombinant of Sclerotinia sclerotiorum Hypovirus 2 Significantly Reduces Virulence of the Fungus

Marzano

Hobbs

Nelson

et al. 2015

J Virol

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A recombinant strain of Sclerotinia sclerotiorum hypovirus 2 (SsHV2) was identified from a North American Sclerotinia sclerotiorum isolate (328) from lettuce (Lactuca sativa L.) by high-throughput sequencing of total RNA. The 5=-and 3=-terminal regions of the genome were determined by rapid amplification of cDNA ends. The assembled nucleotide sequence was up to 92% identical to two recently reported SsHV2 strains but contained a deletion near its 5= terminus of more than 1.2 kb relative to the other SsHV2 strains and an insertion of 524 nucleotides (nt) that was distantly related to Valsa ceratosperma hypovirus 1. This suggests that the new isolate is a heterologous recombinant of SsHV2 with a yet-uncharacterized hypovirus. We named the new strain Sclerotinia sclerotiorum hypovirus 2 Lactuca (SsHV2L) and deposited the sequence in GenBank with accession number KF898354. Sclerotinia sclerotiorum isolate 328 was coinfected with a strain of Sclerotinia sclerotiorum endornavirus 1 and was debilitated compared to cultures of the same isolate that had been cured of virus infection by cycloheximide treatment and hyphal tipping. To determine whether SsHV2L alone could induce hypovirulence in S. sclerotiorum, a full-length cDNA of the 14,538-nt viral genome was cloned. Transcripts corresponding to the viral RNA were synthesized in vitro and transfected into a virus-free isolate of S. sclerotiorum, DK3. Isolate DK3 transfected with SsHV2L was hypovirulent on soybean and lettuce and exhibited delayed maturation of sclerotia relative to virus-free DK3, completing Koch's postulates for the association of hypovirulence with SsHV2L. IMPORTANCEA cosmopolitan fungus, Sclerotinia sclerotiorum infects more than 400 plant species and causes a plant disease known as white mold that produces significant yield losses in major crops annually. Mycoviruses have been used successfully to reduce losses caused by fungal plant pathogens, but definitive relationships between hypovirus infections and hypovirulence in S. sclerotiorum were lacking. By establishing a cause-and-effect relationship between Sclerotinia sclerotiorum hypovirus Lactuca (SsHV2L) infection and the reduction in host virulence, we showed direct evidence that hypoviruses have the potential to reduce the severity of white mold disease. In addition to intraspecific recombination, this study showed that recent interspecific recombination is an important factor shaping viral genomes. The construction of an infectious clone of SsHV2L allows future exploration of the interactions between SsHV2L and S. sclerotiorum, a widespread fungal pathogen of plants. Sclerotinia sclerotiorum (Lib.) de Bary is a cosmopolitan fungal plant pathogen that causes necrotic diseases (e.g., Sclerotinia stem rot) in more than 400 plant species, which result in yield losses in major crops each year (1-3). However, the diseases caused by S. sclerotiorum have not been adequately controlled by conventional technologies thus far (4, 5). A number of mycoviruses have been molecularly characterized and ...

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confidence: 99%

Transfection of Sclerotinia sclerotiorum with In Vitro Transcripts of a Naturally Occurring Interspecific Recombinant of Sclerotinia sclerotiorum Hypovirus 2 Significantly Reduces Virulence of the Fungus

Marzano

Hobbs

Nelson

et al. 2015

J Virol

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“…Na Tabela 1 é apresentada a compilação da classificação dos vírus que infectam fungos fitopatogênicos, adaptada de GHABRIAL; SUZUKI (2009), BOINE (2012, DAS et al (2014), LI et al (2014), XIE;), ZHENG et al (2014, ZIJIN et al (2014) e das informações disponibilizadas no site do ICTV (http://www.ictvonline.org/virusTaxonomy.asp, atualizado em abril/2017).…”

Section: Taxonomia De Micovírusunclassified

“…Esse vírus possui partículas alongado-flexuosas com aproximadamente 1.000 nm de comprimento por 25 a 50 nm de diâmetro, genoma linear de ssRNA de senso negativo com tamanho de 10 Kbp e está relacionado à hipovirulên-cia (LIU et al, 2014).…”

Section: Taxonomia De Micovírusunclassified

Vírus que infectam fungos fitopatogênicos

et al. 2018

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Mycovirus are viruses that infect all taxa of fungi.They are generally cryptic or latent, but they can induce minor or imperceptible changes in hosts. Mycoviruses can interfere with the symptoms induced by phytopathogenic fungi hosts, and in some cases they reduce the virulence of its host. Therefore, they are objects of study since they are potential biocontrol agents and are important tools for knowledge of fungal pathogenesis mechanisms. The aim of this review was to gather literature data concerning general aspects of biology and behavior of mycovirus, focusing some phytopathogenic mycovirus.KEYWORDS: mycovirus; biocontrol; hypovirulence; vegetative incompatibility. RESUMO: Micovírus são vírus que infectam todos os taxa de fungos.São geralmente crípticos (latentes), mas podem causar pequenas ou imperceptíveis alterações no hospedeiro. Nos fungos fitopatogênicos, os vírus podem interferir com os sintomas e, em alguns casos, reduzir a virulência de seu hospedeiro; por esta razão, são objeto de estudo, por serem um potencial agente de biocontrole e por serem ferramentas importantes para o conhecimento sobre os mecanismos de patogênese de fungos. A presente revisão teve o objetivo de reunir os dados de literatura relacionados aos aspectos gerais da biologia e do comportamento dos micovírus presentes em alguns fungos fitopatogênicos. PALAVRAS-CHAVE: micovírus; biocontrole; hipovirulência;incompatibilidade vegetativa.

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“…Based on genome nature, all known RNA mycoviruses were classified into three groups: double-stranded RNA (dsRNA), positivesense single-stranded RNA (ϩssRNA), and the recently discovered negative-sense single-stranded RNA (ϪssRNA) (3,4). Mycoviruses with dsRNA genomes are usually encapsidated in rigid particles and are so far classified into six families, including two newly established families, Megabirnaviridae and Quadriviridae (1,5,6).…”

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Characterization of a Novel Megabirnavirus from Sclerotinia sclerotiorum Reveals Horizontal Gene Transfer from Single-Stranded RNA Virus to Double-Stranded RNA Virus

Wang

Sun

et al. 2015

J Virol

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Mycoviruses have been detected in all major groups of filamentous fungi, and their study represents an important branch of virology. Here, we characterized a novel double-stranded RNA (dsRNA) mycovirus, Sclerotinia sclerotiorum megabirnavirus 1 (SsMBV1), in an apparently hypovirulent strain (SX466) of Sclerotinia sclerotiorum. Two similarly sized dsRNA segments (L1-and L2-dsRNA), the genome of SsMBV1, are packaged in rigid spherical particles purified from strain SX466. The full-length cDNA sequence of L1-dsRNA/SsMBV1 comprises two large open reading frames (ORF1 and ORF2), which encode a putative coat protein and an RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis of the RdRp domain clearly indicates that SsMBV1 is related to Rosellinia necatrix megabirnavirus 1 (RnMBV1). L2-dsRNA/SsMBV1 comprises two nonoverlapping ORFs (ORFA and ORFB) encoding two hypothetical proteins with unknown functions. The 5=-terminal regions of L1-and L2-dsRNA/SsMBV1 share strictly conserved sequences and form stable stem-loop structures. Although L2-dsRNA/SsMBV1 is dispensable for replication, genome packaging, and pathogenicity of SsMBV1, it enhances transcript accumulation of L1-dsRNA/SsMBV1 and stability of virus-like particles (VLPs). Interestingly, a conserved papain-like protease domain similar to a multifunctional protein (p29) of Cryphonectria hypovirus 1 was detected in the ORFA-encoded protein of L2-dsRNA/SsMBV1. Phylogenetic analysis based on the protease domain suggests that horizontal gene transfer may have occurred from a singlestranded RNA (ssRNA) virus (hypovirus) to a dsRNA virus, SsMBV1. Our results reveal that SsMBV1 has a slight impact on the fundamental biological characteristics of its host regardless of the presence or absence of L2-dsRNA/SsMBV1. IMPORTANCEMycoviruses are widespread in all major fungal groups, and they possess diverse genomes of mostly ssRNA and dsRNA and, recently, circular ssDNA. Here, we have characterized a novel dsRNA virus (Sclerotinia sclerotiorum megabirnavirus 1 [SsMBV1]) that was isolated from an apparently hypovirulent strain, SX466, of Sclerotinia sclerotiorum. Although SsMBV1 is phylogenetically related to RnMBV1, SsMBV1 is markedly distinct from other reported megabirnaviruses with two features of VLPs and conserved domains. Our results convincingly showed that SsMBV1 is viable in the absence of L2-dsRNA/SsMBV1 (a potential large satellite-like RNA or genuine genomic virus component). More interestingly, we detected a conserved papain-like protease domain that commonly exists in ssRNA viruses, including members of the families Potyviridae and Hypoviridae. Phylogenetic analysis based on the protease domain suggests that horizontal gene transfer might have occurred from an ssRNA virus to a dsRNA virus, which may provide new insights into the evolutionary history of dsRNA and ssRNA viruses. M ycoviruses (or fungal viruses) with RNA or, rarely, DNA genomes are ubiquitous in plant-pathogenic fungi (1, 2). Based on genome nature, all known RNA mycoviruses...

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Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses

Cited by 191 publications

References 42 publications

Transfection of Sclerotinia sclerotiorum with In Vitro Transcripts of a Naturally Occurring Interspecific Recombinant of Sclerotinia sclerotiorum Hypovirus 2 Significantly Reduces Virulence of the Fungus

Transfection of Sclerotinia sclerotiorum with In Vitro Transcripts of a Naturally Occurring Interspecific Recombinant of Sclerotinia sclerotiorum Hypovirus 2 Significantly Reduces Virulence of the Fungus

Vírus que infectam fungos fitopatogênicos

Characterization of a Novel Megabirnavirus from Sclerotinia sclerotiorum Reveals Horizontal Gene Transfer from Single-Stranded RNA Virus to Double-Stranded RNA Virus

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