Fungal Pretreatment of Corn Stover by Fomes sp. EUM1: Simultaneous Production of Readily Hydrolysable Biomass and Useful Biocatalysts

Hernández, Jazmín Edith Méndez; Loera, Octavio; Méndez-Hernández, Edna M.; Herrera, Esperanza Tamariz; Arce-Cervantes, Oscar; Soto-Cruz, N.O.

doi:10.1007/s12649-018-0290-1

Cited by 6 publications

(7 citation statements)

References 43 publications

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“…Biological pretreatments, such as SSF, have also been used. For example, Méndez-Hernández et al (2019) subjected the corn stover to a biological pretreatment using a thermotolerant fungus, Fomes sp., and observed a 60% improvement of sugars released after 7 days in comparison to the untreated corn stover. The SSF followed by the enzymatic hydrolysis of a green macroalgae ( Ulva rigida ) with enzymes produced during the SSF increased the glucose yield by 53% ( Fernandes et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Valorization of Brewer’s Spent Grain Using Biological Treatments and its Application in Feeds for European Seabass (Dicentrarchus labrax)

Fernandes

Salgado

Ferreira

et al. 2022

Front. Bioeng. Biotechnol.

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Brewer’s spent grain (BSG) is the main brewery industry by-product, with potential applications in the feed and food industries due to its carbohydrate composition. In addition, the lignocellulosic nature of BSG makes it an adequate substrate for carbohydrases production. In this work, solid-state fermentation (SSF) of BSG was performed with Aspergillus ibericus, a non-mycotoxin producer fungus with a high capacity to hydrolyze the lignocellulosic matrix of the agro-industrial by-products. SSF was performed at different scales to produce a crude extract rich in cellulase and xylanase. The potential of the crude extract was tested in two different applications: -(1) - the enzymatic hydrolysis of the fermented BSG and (2) - as a supplement in aquafeeds. SSF of BSG increased the protein content from 25% to 29% (w/w), while the fiber content was reduced to 43%, and cellulose and hemicellulose contents were markedly reduced to around 15%. The scale-up of SSF from 10 g of dry BSG in flasks to 50 g or 400 g in tray-type bioreactors increased 55% and 25% production of cellulase and xylanase, up to 323 and 1073 U g−1 BSG, respectively. The optimum temperature and pH of maximal activities were found to be 55°C and pH 4.4 for xylanase and 50°C and pH 3.9 for cellulase, cellulase being more thermostable than xylanase when exposed at temperatures from 45°C to 60°C. A Box–Behnken factorial design was applied to optimize the hydrolysis of the fermented BSG by crude extract. The crude extract load was a significant factor in sugars release, highlighting the role of hydrolytic enzymes, while the load of fermented BSG, and addition of a commercial β-glucosidase were responsible for the highest phenolic compounds and antioxidant activity release. The lyophilized crude extract (12,400 and 1050 U g−1 lyophilized extract of xylanase and cellulase, respectively) was also tested as an enzyme supplement in aquafeed for European seabass (Dicentrarchus labrax) juveniles. The dietary supplementation with the crude extract significantly improved feed and protein utilization. The processing of BSG using biological treatments, such as SSF with A. ibericus, led to the production of a nutritionally enriched BSG and a crude extract with highly efficient carbohydrases capable of hydrolyzing lignocellulosic substrates, such as BSG, and with the potential to be used as feed enzymes with remarkable results in improving feed utilization of an important aquaculture fish species.

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Section: Discussionmentioning

confidence: 99%

Valorization of Brewer’s Spent Grain Using Biological Treatments and its Application in Feeds for European Seabass (Dicentrarchus labrax)

Fernandes

Salgado

Ferreira

et al. 2022

Front. Bioeng. Biotechnol.

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“…Laccase activity reached its peak of 336.36 U/gds after 4 days, with the lowest activity levels of 53.71 U/gds observed after 60 days. The decline in laccase productivity after 4 days may be attributed to the gradual secretion of proteases [28]. Interestingly, a second peak in laccase levels was observed, with a rapid increase reaching 166.94 U/gds after 40 days of incubation.…”

Section: Enzyme Quantification and Correlation With The Biomass Cellu...mentioning

confidence: 94%

“…For instance, Saha et al [8] investigated the productivity rates of different lignocellulolytic enzymes when utilizing P. revisor NRRL-13108 and corn stover as substrates, and found that laccase and xylanase reached peak productivities after 8 and 27 days, respectively, with no observable mannanase activity. Méndez-Hernández et al [28] explored the utilization of Fomes sp. EUM1 for enzyme production during the biological pretreatment of corn stover and reported that laccase reached a maximum productivity of 4.2 U/g after 7 days, whereas xylanase peaked at 77.4 U/g after 5 days of treatment.…”

Section: Microorganismmentioning

confidence: 99%

“…Microbial treatment offers targeted action on the lignin-hemicellulose complex and provides the advantage of treated biomass with improved hydrolysis activities and a variety of ligno, hemicellulo, or cellulolytic enzyme systems. The productivity profiles of ligno-hemicellulolytic enzymes are critical in determining the efficiency of the biological pretreatment reaction [10,28,30].…”

Section: Enzyme Quantification and Correlation With The Biomass Cellu...mentioning

confidence: 99%

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Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover

Rastogi¹,

Soni²,

Sharma³

et al. 2023

Preprint

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Biological pretreatment of lignocellulosic residues has the potential to serve as a sustainable, less energy-intensive alternative to harsh chemical treatments for enhancing cellulose accessibility, despite the requirement of a lengthy incubation period. The study characterized the simultaneous delignification of corn stover by the white-rot fungus Pyrenophora phaeocomes S-1, as well as the co-production of a ligno-hemicellulolytic enzyme cocktail consisting of laccase, xylanase, and mannanase. The maximum yields of all three components of the cocktail were achieved after just 4 days of incubation under solid-state conditions. Following a 40-day fermentation period, we achieved a cellulose recovery of 44.25 ± 1.72%, attributed to the activities of 133.88 U/gds of laccase, 14.93 U/gds of xylanase, and 1.34 U/gds of mannanase co-produced by the fungus in the medium. Subsequently, through the extraction of biologically treated biomass with 0.5N NaOH after 40 days, the cellulose recovery increased to 66.4 ± 1.39%. Enzymatic hydrolysis of the same, employing only 5 FPU/gds of in-house produced cellulases, resulted in the liberation of 397.84 mg/gds of total reducing sugars after 144h. The findings of this study encourage further optimization of biological pretreatment of lignocellulosic residues and enzymatic hydrolysis to enhance the yields of total reducing sugars for their valorization.

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“…Corn (Zea Maize L.) is the most important crop at a global level in terms of annual production (Shiferaw et al, 2011). In 2015, corn production in the United States exceeded 250 million tons (USDA-NASS, 2016;Méndez-Hernández et al, 2019). The interest in corn residues lies in the fact that for every 100 kg of corn grain obtained, ∼18 kg are represented by corncobs, which mostly remain unused as lignocellulosic waste (Torre et al, 2008;Lau et al, 2019).…”

mentioning

confidence: 99%

Extraction of high-quality grade cellulose and cellulose nanocrystals from different lignocellulosic agri-food wastes

Rovera

Carullo

Bellesia

et al. 2023

Front. Sustain. Food Syst.

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IntroductionPrompted by the increasing need for an intensified valorization of agri-food waste, in this work a three-step chemical procedure was used to extract high-purity cellulose from garlic stalk, corncob, and giant cane cut-up by a sequential removal of hemicellulose, lignin, ash, and organic compounds. Cellulose nanocrystals of potential interest for nanocomposite applications were then obtained through acid hydrolysis.MethodsThe purity of the cellulose was determined employing Nuclear Magnetic Resonance and infrared spectroscopy, whereas dynamic light scattering, optical, atomic force microscopy, and transmission electron microscopy were used for morphological characterization. The high purity and crystallinity of cellulose was confirmed by comparison with the ultra-pure bacterial cellulose originating from K. sucrofermentans, irrespective of the waste used.Results and discussionAt the end of the extraction procedure, cellulose yields of 35.73, 37.15, and 39.10%, for garlic stalk, corncob, and giant cane cut-up, respectively, were achieved. Dynamic light scattering and atomic force microscopy analyses showed that the length of the whisker-like nanocrystals depended on the raw material (from ~100 nm up to > 2μm), while the final yield was ~40–50% for all three wastes. The versatility and effectiveness of the method here proposed can be profitably used for a wide range of agro-waste feedstocks.

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Fungal Pretreatment of Corn Stover by Fomes sp. EUM1: Simultaneous Production of Readily Hydrolysable Biomass and Useful Biocatalysts

Cited by 6 publications

References 43 publications

Valorization of Brewer’s Spent Grain Using Biological Treatments and its Application in Feeds for European Seabass (Dicentrarchus labrax)

Valorization of Brewer’s Spent Grain Using Biological Treatments and its Application in Feeds for European Seabass (Dicentrarchus labrax)

Unleashing the Ligno-Hemicellulolytic Enzyme Potential of Pyrenophora phaeocomes S-1: A Solid State Fermentation Approach for Efficient Delignification of Corn Stover

Extraction of high-quality grade cellulose and cellulose nanocrystals from different lignocellulosic agri-food wastes

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