Fungal pretreatment of sugarcane bagasse: a green pathway to improve saccharification and ethanol production

Hartmann, Caroline; Fontana, Roselei Claudete; Siqueira, Félix Gonçalves de; Camassola, Marli

doi:10.1007/s12155-021-10329-6

Cited by 7 publications

(2 citation statements)

References 53 publications

(43 reference statements)

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“…The treatment of rice straw with 10 IU/g enzyme (CMCase) at 30,40 and 50 ºC temperature for 24 and 48 h on rotatory shaker revealed that Penicillium chrysogenum released highest amount of reducing sugar followed by Aspergillus flavus, A. oryzae, A. fumigatus and Trichoderma viride (Table 1). The release of reducing sugars further increased with increasing temperature and duration of incubation that suggest that the enzyme complex released by the microfungi is not de-activated on longer periods of incubation and increasing temperature [35,36,37]. Further the experiments were designed to evaluate the degradation potential of Penicillium chrysogenum, Aspergillus flavus and A. oryzae singly and in combination of each other under field conditions.…”

Section: Resultsmentioning

confidence: 99%

Rice Straw Degradation with Mixed Cultures of Microfungi and Their Enzymes

Sahrawat

Garg

2023

IJECC

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Huge quantities of agricultural residues are generated every year but it is neither converted into energy nor allowed to go back to the soil and sometimes burnt that leads to air pollution and loss of soil biology and fertility. Cellulolytic microfungi secrete extracellular enzymes that degrade lignocellulosic biomass in nature and this ability of microbes may be exploited to enhance the rates of degradation of agriculture residues to recycle carbon, nitrogen and minerals in the soil. This study aimed to find out the effect(s) of co-cultivation of high cellulolytic microfungi in various combinations on rice straw under field conditions to enhance its microbial decomposition to discourage the farmers from burning it in the fields. The effectiveness of five fungal cultures in different combinations was tested for efficient degradation of rice straw. Five dominant species of fungi that have been earlier shown to secrete high amounts of cellulases in our own laboratories, were cultured on medium containing yeast powder (2g/L), jaggery (5g/L) and urea (1g/L) at room temperature. Release of reducing sugars from 1 g of rice straw treated with 10IU/mL of fungal extracellular enzymes showed that Penicillium chrysogenum released highest amount of mono and oligomers (96 mg/g) followed by Aspergillus flavus (80 mg/g) and A. oryzae (78 mg/g), A. fumigatus (72 mg/g) and Trichoderma viride (70 mg/g) within 24 h that increased with increasing temperature and increasing period of incubation. Treatment of rice straw with fungal cultures of Penicillium chrysogenum, Aspergillus flavus and A. oryzae revealed that the co-inoculation of all these species decomposed approximately 75% of the total rice straw as assessed by weight loss method. The application extracellular secretory enzymes on rice straw, though, revealed to release of reducing sugars, but the rate of reducing sugars was not sufficient enough to be used for degradation of rice straw under field conditions, hence, the used of mixed cultures of microfungi was planned and tested experimentally, which allowed the decomposition of rice straw much faster than control which were treated with heat-killed dead cultures of test microfungi. Hence, it is recommended that additional spray of mixed cultures of microfungi on rice straw may facilitate its degradation under field conditions.

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Section: Resultsmentioning

confidence: 99%

Rice Straw Degradation with Mixed Cultures of Microfungi and Their Enzymes

Sahrawat

Garg

2023

IJECC

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“…To date, few studies evaluating the biological pretreatment of SCB have been reported [84]. However, some studies have shown that the use of fungi enhances the digestibility of polysaccharides, while very few microorganisms are able to fully decompose lignin in SCB [115][116][117]. Microbial consortium pretreatment has been used on lignocellulosic biomass to increase biogas production [118,119].…”

Section: Pretreatment Of Sugarcane Bagassementioning

confidence: 99%

Valorisation of Sugarcane Bagasse for the Sustainable Production of Polyhydroxyalkanoates

Hassan,

Ngo,

Ball

2024

Sustainability

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With the world shifting towards renewable and sustainable resources, polyhydroxyalkanoates (PHAs) have attracted significant interest as an alternative to synthetic plastics. While possessing promising properties suitable for various applications, the production of PHAs has not yet reached a global commercial scale. The main reason is the high cost of production, which represents a major limitation. Sugarcane bagasse (SCB) is an abundant lignocellulosic waste around the world. Its use to produce PHA enhances the feasibility of producing PHAs at commercial scale. However, SCB requires pretreatment and hydrolysis steps to release the sugars prior to the microbial fermentation. The cost associated with these steps poses additional challenges for large-scale production. Another challenge is the release of inhibitors during the pretreatment process which can result in a low PHA yield. The development of a low cost, co-culture strategy for the bioconversion of SCB into PHAs, can represent a pivotal step towards the large-scale production of bioplastics. This review highlights the advancements made in recent years on the microbial production of PHA using SCB as potential feedstock, with a proposed biological strategy and circular economy model.

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