Fungal production of eicosapentaenoic and arachidonic acids from industrial waste streams and crude soybean oil

Cheng, Ming‐Hsun; Walker, Terry; Hulbert, Gregory J.; Raman, D. Raj

doi:10.1016/s0960-8524(98)00113-8

Cited by 62 publications

(27 citation statements)

References 31 publications

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“…The highest products of EPA and ARA were in C/N = 32 (2% glucose and 0.25% yeast extract-20 mg /g and 15.3 mg /g. The results were similar with the results of Cheng (43).…”

Section: Discussionsupporting

confidence: 90%

Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids

Ghasemi¹

2015

AJLS

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Abstract:Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma-linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in "temperature shifting technique", greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH 2 PO 4 at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.

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“…The highest products of EPA and ARA were in C/N = 32 (2% glucose and 0.25% yeast extract-20 mg /g and 15.3 mg /g. The results were similar with the results of Cheng (43).…”

Section: Discussionsupporting

confidence: 90%

Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids

Ghasemi¹

2015

AJLS

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“…1C, respectively). Thus, taking into account that depletion of nutrients in the medium results in a metabolic shift towards lipid accumulation and EPA production (Cheng et al 1999;Koike et al 2001;Zhu 2002;, these correlations indicate that cultures with low EPA levels lacked sufficient activity of the conversion enzymes or were not sufficiently stressed by nutrient and lipid exhaustion to switch to HUFA and EPA production. Also, the negative correlation between lipid and EPA content (Figs 1B,C) indicates that optimization for EPA production using SSF with Mortierella, growing on LSO enriched BSG, will most probably result in a trade off between lipid and EPA content of the culture.…”

Section: Resultsmentioning

confidence: 99%

“…Despite this, the use of SSF for the production of HUFA has received less attention than submerged fermentation, due to technical problems, such as contamination control, scaling-up and lipid extraction (Zhu 2002). Traditionally agricultural products like cereals, soybeans or rice have been used as solid substrates, but the use of inexpensive agricultural, industrial or food-processing wastes is attracting increasing attention among researchers (Cheng et al 1999;Stredansky et al 2000). The South African beer brewing industry produces approximately 290 000 tons of brewers' spent grain (BSG) per year (http://www.sablimited.co.za/).…”

Section: Introductionmentioning

confidence: 99%

The production of eicosapentaenoic acid by representatives of the genus Mortierella grown on brewers’ spent grain

2009

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Brewers' spent grain (BSG) was evaluated as substrate for the production of eicosapentaenoic acid (EPA) by solid-state fermentation with 29 fungal strains representing different Mortierella species. The effect of a 10% (w/w) linseed oil (LSO) supplement on EPA production was also determined. All the strains produced EPA on the substrate, while addition of the LSO improved the EPA yield of most strains. The strains producing the most EPA in the absence of additional LSO generally also produced the most EPA when LSO was added to the BSG. The strains, which produced the highest levels of EPA on BSG supplemented with LSO were Mortierella antarctica Mo 67 and Mortierella epicladia Mo 101, which respectively produced 2.8 mg and 2.5 mg EPA per g of BSG.

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“…Oleic acid in particular is abundant in skin cells and is a very good medium for transferring nutrients across the skin barrier. Oils rich in oleic acid are generally very absorbent and as a result are in wide use in the skin care industry (Cheng et al 1999).…”

Section: Revitalizing Skin and Anti-agingmentioning

confidence: 99%

Fungi—an unusual source for cosmetics

2010

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Fruiting bodies of some wild and cultivatable mushrooms contain medicinal compounds which are being used in traditional medicines and cosmetics. There are numerous potential medicinal products from mushrooms that could be used in cosmeceuticals (products applied topically, such as creams, lotions, and ointments) or nutricosmetics (products that are ingested orally). This paper provides a review of the fungi presently used in cosmeceuticals and nutricosmetics with some examples of cosmetic types and products. Species presently used, or patented to be used, in cosmeceuticals and nutricosmetics include Agaricus subrufescens (= A. blazei, A. brasiliensis) Choiromyces maeandriformis Cordyceps sinensis, Ganoderma lucidum, Grifola frondosa, Hypsizygus ulmarium, Inonotus obliquus, Lentinula edodes, Polyporus spp., Trametes versicolor, Tremella fuciformis, Tuber spp., Schizophyllum commune and many other lesser used taxa. Cosmetics incorporating fungi include those for skin care such as anti-aging, anti-oxidants, skin revitalizing, skin whitening and hair products. The mushrooms presently used are traditionally known to produce medicinal compounds and thus were the first to be incorporated in cosmetic applications. There are, however, numerous other mushroom species that are untested, undescribed or not yet cultivatable and that have huge potential for use in the cosmetic industry. Some fungi are also used in biotransformation and the products such as lactic acid and ceramides could potentially be used in cosmetics.

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Fungal production of eicosapentaenoic and arachidonic acids from industrial waste streams and crude soybean oil

Cited by 62 publications

References 31 publications

Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids

Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids

The production of eicosapentaenoic acid by representatives of the genus Mortierella grown on brewers’ spent grain

Fungi—an unusual source for cosmetics

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