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Fungi are a distinguished category of microorganisms that belong to a distinct kingdom. Biotechnological methods have significant contributions in several domains and shown generosity in manufacturing diverse metabolites that belong to distinct chemical classes and possess varying bioactivity and/or industrial applications. The mentioned advantages have fostered the habit of screening for fungus from unexplored origins. In this study, we conducted a screening to identify fungus from a distant bat cave in Assiut governorate, Egypt. Specifically, we focused on endophytic fungi found in flower petals and endolichenic fungi found in lichen. As a consequence, 17 distinct fungal species were isolated, which can be classified into 12 different fungal genera. Furthermore, the morphological and microscopic identification of the 4 isolates was unattainable due to their sterile mycelia appearance. Therefore, four individual specimens were genetically identified by sequencing their ITS regions as Rhizomucor miehei NRCWA, Penicillium chrysogenum NRCWA, P. polonicum NRCWA, and P. crustosum NRCWA. The corresponding accession codes for these identifications are PP422033, PP422038, PP422039, and PP422040, respectively. This study is the initial documentation of the extraction of endophytic and endolichenic fungi from Chrysothrix candelaris specimens obtained from Egypt. Exploring and identifying fungi from unexplored sources can help uncover powerful metabolites that have potential applications in several biotechnological and industrial sectors.
Fungi are a distinguished category of microorganisms that belong to a distinct kingdom. Biotechnological methods have significant contributions in several domains and shown generosity in manufacturing diverse metabolites that belong to distinct chemical classes and possess varying bioactivity and/or industrial applications. The mentioned advantages have fostered the habit of screening for fungus from unexplored origins. In this study, we conducted a screening to identify fungus from a distant bat cave in Assiut governorate, Egypt. Specifically, we focused on endophytic fungi found in flower petals and endolichenic fungi found in lichen. As a consequence, 17 distinct fungal species were isolated, which can be classified into 12 different fungal genera. Furthermore, the morphological and microscopic identification of the 4 isolates was unattainable due to their sterile mycelia appearance. Therefore, four individual specimens were genetically identified by sequencing their ITS regions as Rhizomucor miehei NRCWA, Penicillium chrysogenum NRCWA, P. polonicum NRCWA, and P. crustosum NRCWA. The corresponding accession codes for these identifications are PP422033, PP422038, PP422039, and PP422040, respectively. This study is the initial documentation of the extraction of endophytic and endolichenic fungi from Chrysothrix candelaris specimens obtained from Egypt. Exploring and identifying fungi from unexplored sources can help uncover powerful metabolites that have potential applications in several biotechnological and industrial sectors.
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