Fungi in PAH-contaminated marine sediments: Cultivable diversity and tolerance capacity towards PAH

Álvarez-Barragán, Joyce; Cravo‐Laureau, Cristiana; Wick, Lukas Y.; Duran, Robert

doi:10.1016/j.marpolbul.2021.112082

Cited by 28 publications

(21 citation statements)

References 98 publications

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“…Potential carbon sources for fungi in marine sediments that are accessed via exoenzyme secretion include refractory polysaccharides [ 101 ] and refractory carbohydrates (e.g., beta-glucans) [ 102 ]. Fungi take part in degradation of hydrocarbons in soils and sediments, including aromatic compounds [ 103 – 105 ]. Physiologically versatile fungi persist in the deep subsurface biosphere, and have been cultivated from coal-bearing deep subsurface sediments at 2 km depth [ 106 , 107 ].…”

Section: Discussionmentioning

confidence: 99%

Environmental factors shaping bacterial, archaeal and fungal community structure in hydrothermal sediments of Guaymas Basin, Gulf of California

et al. 2021

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The flanking regions of Guaymas Basin, a young marginal rift basin located in the Gulf of California, are covered with thick sediment layers that are hydrothermally altered due to magmatic intrusions. To explore environmental controls on microbial community structure in this complex environment, we analyzed site- and depth-related patterns of microbial community composition (bacteria, archaea, and fungi) in hydrothermally influenced sediments with different thermal conditions, geochemical regimes, and extent of microbial mats. We compared communities in hot hydrothermal sediments (75-100°C at ~40 cm depth) covered by orange-pigmented Beggiatoaceae mats in the Cathedral Hill area, temperate sediments (25-30°C at ~40 cm depth) covered by yellow sulfur precipitates and filamentous sulfur oxidizers at the Aceto Balsamico location, hot sediments (>115°C at ~40 cm depth) with orange-pigmented mats surrounded by yellow and white mats at the Marker 14 location, and background, non-hydrothermal sediments (3.8°C at ~45 cm depth) overlain with ambient seawater. Whereas bacterial and archaeal communities are clearly structured by site-specific in-situ thermal gradients and geochemical conditions, fungal communities are generally structured by sediment depth. Unexpectedly, chytrid sequence biosignatures are ubiquitous in surficial sediments whereas deeper sediments contain diverse yeasts and filamentous fungi. In correlation analyses across different sites and sediment depths, fungal phylotypes correlate to each other to a much greater degree than Bacteria and Archaea do to each other or to fungi, further substantiating that site-specific in-situ thermal gradients and geochemical conditions that control bacteria and archaea do not extend to fungi.

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Section: Discussionmentioning

confidence: 99%

Environmental factors shaping bacterial, archaeal and fungal community structure in hydrothermal sediments of Guaymas Basin, Gulf of California

et al. 2021

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“…PAHs released into the environment change their state mainly depending on photoconversion or biodegradation [ 31 , 32 ]. Photodegradation is the main pathway for abiotic conversion of most PAHs in the environment [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Potential Toxicity Risk Assessment and Priority Control Strategy for PAHs Metabolism and Transformation Behaviors in the Environment

Zhao

Zhou

Zhao

et al. 2022

IJERPH

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In this study, 16 PAHs were selected as the priority control pollutants to summarize their environmental metabolism and transformation processes, including photolysis, plant degradation, bacterial degradation, fungal degradation, microalgae degradation, and human metabolic transformation. Meanwhile, a total of 473 PAHs by-products generated during their transformation and degradation in different environmental media were considered. Then, a comprehensive system was established for evaluating the PAHs by-products’ neurotoxicity, immunotoxicity, phytotoxicity, developmental toxicity, genotoxicity, carcinogenicity, and endocrine-disrupting effect through molecular docking, molecular dynamics simulation, 3D-QSAR model, TOPKAT method, and VEGA platform. Finally, the potential environmental risk (phytotoxicity) and human health risks (neurotoxicity, immunotoxicity, genotoxicity, carcinogenicity, developmental toxicity, and endocrine-disrupting toxicity) during PAHs metabolism and transformation were comprehensively evaluated. Among the 473 PAH’s metabolized and transformed products, all PAHs by-products excluding ACY, CHR, and DahA had higher neurotoxicity, 152 PAHs by-products had higher immunotoxicity, and 222 PAHs by-products had higher phytotoxicity than their precursors during biological metabolism and environmental transformation. Based on the TOPKAT model, 152 PAH by-products possessed potential developmental toxicity, and 138 PAH by-products had higher genotoxicity than their precursors. VEGA predicted that 247 kinds of PAH derivatives had carcinogenic activity, and only the natural transformation products of ACY did not have carcinogenicity. In addition to ACY, 15 PAHs produced 123 endocrine-disrupting substances during metabolism and transformation. Finally, the potential environmental and human health risks of PAHs metabolism and transformation products were evaluated using metabolic and transformation pathway probability and degree of toxic risk as indicators. Accordingly, the priority control strategy for PAHs was constructed based on the risk entropy method by screening the priority control pathways. This paper assesses the potential human health and environmental risks of PAHs in different environmental media with the help of models and toxicological modules for the toxicity prediction of PAHs by-products, and thus designs a risk priority control evaluation system for PAHs.

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“…Initial pH of the medium was adjusted to 7.0-7.2 with sterile 10 M NaOH. Filter sterilized trace element and V7 solutions were added to the sterile MSM as described in previous studies (Kovach et al, 1995;Alvarez-Barragan et al, 2021). The composition of the lysogeny broth (LB) is as follows (g/L): tryptone, 10; yeast extract, 5; NaCl, 10 (Sambrook, 2001).…”

Section: Bacterial Strains and Culture Mediamentioning

confidence: 99%

“…For the microorganisms from the marine samples, MSM (Dias et al, 2008;Alvarez-Barragan et al, 2021) containing 4 g/l sodium acetate as carbon source with 1% agarose was used to fill the wells of the device. The microbial suspension was adjusted to OD 0.1 at 560 nm and diluted 10,000 times before inoculation.…”

Section: Inoculation and Cultivation Of Bacteriamentioning

confidence: 99%

Low-cost gel-filled microwell array device for screening marine microbial consortium

et al. 2022

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In order to exploit the microbes present in the environment for their beneficial resources, effective selection and isolation of microbes from environmental samples is essential. In this study, we fabricated a gel-filled microwell array device using resin for microbial culture. The device has an integrated sealing mechanism that enables high-density isolation based on the culture of microorganisms; the device is easily manageable, facilitating observation using bright-field microscopy. This low-cost device made from polymethyl methacrylate (PMMA)/polyethylene terephthalate (PET) has 900 microwells (600 μm × 600 μm × 700 μm) filled with a microbial culture gel medium in glass slide-sized plates. It also has grooves for maintaining the moisture content in the micro-gel. The partition wall between the wells has a highly hydrophobic coating to inhibit microbial migration to neighboring wells and to prevent exchange of liquid substances. After being hermetically sealed, the device can maintain moisture in the agarose gels for 7 days. In the bacterial culture experiment using this device, environmental bacteria were isolated and cultured in individual wells after 3 days. Moreover, the isolated bacteria were then picked up from wells and re-cultured. This device is effective for the first screening of microorganisms from marine environmental samples.

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Fungi in PAH-contaminated marine sediments: Cultivable diversity and tolerance capacity towards PAH

Cited by 28 publications

References 98 publications

Environmental factors shaping bacterial, archaeal and fungal community structure in hydrothermal sediments of Guaymas Basin, Gulf of California

Environmental factors shaping bacterial, archaeal and fungal community structure in hydrothermal sediments of Guaymas Basin, Gulf of California

Potential Toxicity Risk Assessment and Priority Control Strategy for PAHs Metabolism and Transformation Behaviors in the Environment

Low-cost gel-filled microwell array device for screening marine microbial consortium

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