Fungicidal activity of human lactoferrin-derived peptides based on the antimicrobial αβ region

Kondori, Nahid; Baltzer, Lars; Dolphin, Gunnar T.; Mattsby‐Baltzer, Inger

doi:10.1016/j.ijantimicag.2010.08.020

Cited by 46 publications

(40 citation statements)

References 27 publications

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“…The protein at concentrations of 12.5 μ g/mL (2xMIC) was added to the cell suspension and was incubated at different time intervals at 35°C. High concentration of test sample is chosen in order to achieve killing of a high number of yeast cells [9]. The cells were prepared for SEM study by treatment with 1% osmium tetroxide for 1 h at 4°C [10].…”

Section: Methodsmentioning

confidence: 99%

Antifungal and Antiproliferative Protein fromCicer arietinum: A Bioactive Compound against Emerging Pathogens

Kumar

Kapoor

Gill

et al. 2014

BioMed Research International

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The emergence of epidemic fungal pathogenic resistance to current antifungal drugs has increased the interest in developing alternative antibiotics from natural sources. Cicer arietinum is well known for its medicinal properties. The aim of this work was to isolate antimicrobial proteins from Cicer arietinum. An antifungal protein, C-25, was isolated from Cicer arietinum and purified by gel filtration. C-25 protein was tested using agar diffusion method against human pathogenic fungi of ATCC strains and against clinical isolates of Candida krusei, Candida tropicalis, and Candida parapsilosis, and MIC values determined were varied from 1.56 to 12.5 μg/mL. The SEM study demonstrated that C-25 induces the bleb-like surface changes, irregular cell surface, and cell wall disruption of the fungi at different time intervals. Cytotoxic activity was studied on oral cancer cells and normal cells. It also inhibits the growth of fungal strains which are resistant to fluconazole. It reduced the cell proliferation of human oral carcinoma cells at the concentration of 37.5 μg/mL (IC50) and no toxic effect was found on normal human peripheral blood mononuclear cells even at higher concentration of 600 μg/mL. It can be concluded that C-25 can be considered as an effective antimycotic as well as antiproliferative agent against human oral cancer cells.

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Section: Methodsmentioning

confidence: 99%

Antifungal and Antiproliferative Protein fromCicer arietinum: A Bioactive Compound against Emerging Pathogens

Kumar

Kapoor

Gill

et al. 2014

BioMed Research International

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“…Además, la participación eficiente del sistema inmunitario se sustentó con los estudios epidemiológicos en áreas endémicas, donde se reportó que hasta el 80% de los individuos presentan anticuerpos, siendo el número de enfermos comparativamente bajo. La evidencia señala que los pacientes que desarrollan la enfermedad presentan falla en la inmunidad innata 24,25 . En pacientes infectados por M. mycetomatis se estudiaron varios genes involucrados en la función de los neutrófilos y se encontró desequilibrio de Hardy-Weinberg para polimorfismos de un nucleótido (SNP), en el receptor del complemento 1, interleucina 8 (IL-8) y sintasa del óxido nítrico tipo 2 (NOS2), que se correlacionan con una mayor expresión de IL-8 y una baja secreción de NOS2 en los pacientes, mientras que en los controles sanos se encontraron SNP que condicionan baja expresión de IL-8 y alta producción de NOS2 26 .…”

Section: Fisiopatologíaunclassified

Eumicetoma: actualidades y perspectivas

Arenas

Vega-Memije

Rangel-Gamboa

2017

GMM

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“…Haversen et al (107) showed that a 14-amino acid fragment from the N-terminus of human lactoferrin was highly active against E. coli, S. aureus, and C. albicans. Interestingly, it was found that the optimal length for the antimicrobial activity of the lactoferrinderived sequence is 12 aa (amino acids 19-31), but 13 aa (amino acids [17][18][19][20][21][22][23][24][25][26][27][28][29][30] was optimal for amebicidal properties (108)(109)(110). Recently, a new strategy to identify putative AMPs encrypted in protein sequences has been described.…”

Section: Natural Amp-derived Fragments and Design Of Hybrid Peptidesmentioning

confidence: 99%

Defense peptides: recent developments

Cytryńska

Zdybicka-Barabas

2015

Biomolecular Concepts

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Defense peptides are small amphipathic molecules that exhibit antimicrobial, antitumor, antiviral, and immunomodulatory properties. This review summarizes current knowledge on the mechanisms of antimicrobial activity of cationic and anionic defense peptides, indicating peptide-based as well as microbial cell-based factors affecting this activity. The peptide-based factors include charge, hydrophibicity, and amphipathicity, whereas the pathogen-based factors are membrane lipid composition, presence of sterols, membrane fluidity, cell wall components, and secreted factors such as extracellular proteinases. Since defense peptides have been considered very promising molecules that could replace conventional antibiotics in the era of drug-resistant pathogens, the issue of microbial resistance to antimicrobial peptides (AMPs) is addressed. Furthermore, selected approaches employed for optimization and de novo design of effective AMPs based on the properties recognized as important for the function of natural defense peptides are presented.

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Fungicidal activity of human lactoferrin-derived peptides based on the antimicrobial αβ region

Cited by 46 publications

References 27 publications

Antifungal and Antiproliferative Protein fromCicer arietinum: A Bioactive Compound against Emerging Pathogens

Antifungal and Antiproliferative Protein fromCicer arietinum: A Bioactive Compound against Emerging Pathogens

Eumicetoma: actualidades y perspectivas

Defense peptides: recent developments

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